Cell apoptosis, sometimes called programmed cell death, is a cellular self-destruction method to remove old and damaged cells during development and aging to protect cells from external disturbances and maintain homeostasis. Apoptosis also occurs as a defense mechanism such as in immune reactions or when cells are damaged by disease or noxious agents.

Apoptosis is controlled by many genes and involves two fundamental pathways: the extrinsic pathway, which transmits death signals by the death receptor (DR), and the intrinsic or mitochondrial pathway. The extrinsic apoptotic pathway is activated by the binding of the death ligand to DRs, including FasL, TNF-α, and TRAIL, on the plasma membrane. The DR, adaptor protein (FADD), and associated apoptosis signaling molecule (caspase-8) form the death-inducing signaling complex (DISC), thus leading to the activation of the effector caspase cascade (caspase-3, -6, and -7). The mitochondria-mediated intrinsic apoptosis pathway is regulated by Bcl-2 family proteins, including proapoptotic (Bid, Bax, Bak) and antiapoptotic proteins (Bcl-2, Bcl-xL).

Abnormalities in cell apoptosis can be a significant component of diseases such as cancer, autoimmune lymphoproliferative syndrome, AIDS, ischemia, and neurode-generative diseases. These diseases may benefit from artificially inhibiting or activating apoptosis. A short list of potential methods of anti-apoptotic therapy includes stimulation of the IAP (inhibitors of apoptosis proteins) family of proteins, caspase inhibition, PARP (poly [ADP-ribose] polymerase) inhibition, stimulation of the PKB/Akt (protein kinase B) pathway, and inhibition of Bcl-2 proteins.

Ferroptosis and necroptosis are recently recognized forms of regulated cell death that differs considerably from apoptosis. Misregulated ferroptosis or necroptosis have also been implicated in multiple physiological and pathological processes, including cancer cell death, neurotoxicity, neurodegenerative diseases, etc.

References:
[1] Susan Elmore. Toxicol Pathol. 2007; 35(4): 495–516.
[2] Cao L, et al. J Cell Death. 2016 Dec 29;9:19-29.
[3] Dasgupta A, et al. Int J Mol Sci. 2017 Jan; 18(1): 23.
[4] Xie Y, et al. Cell Death Differ. 2016 Mar;23(3):369-79.


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Gliotoxin

Gliotoxin is a secondary metabolite, the most abundant mycotoxin secreted by A. fumigatus, inhibits the phagocytosis of macrophages and the immune functions of other immune cells [1]. Gliotoxin inhibits inducible NF-κB activity by preventing IκB degradation, which consequently induces host-cell apoptosis[2]. Gliotoxin activates PKA and increases intracellular cAMP concentration; modulates actin cytoskeleton rearrangement to facilitate A. fumigatus internalization into lung epithelial cells[3].

  • CAS Number: 67-99-2
  • MF: C13H14N2O4S2
  • MW: 326.391
  • Catalog: Apoptosis
  • Density: 1.8±0.1 g/cm3
  • Boiling Point: 699.7±55.0 °C at 760 mmHg
  • Melting Point: 153.5ºC
  • Flash Point: 377.0±31.5 °C

BDA-366

BDA-366 is a potent Bcl2 antagonist (Ki = 3.3 nM), binding Bcl2-BH4 domain with high affinity and selectivity. BDA-366 induces conformational change in Bcl2 that abrogates its antiapoptotic function, converting it from a survival molecule to a cell death inducer. BDA-366 suppresses growth of lung cancer cells[1].

  • CAS Number: 1909226-00-1
  • MF: C24H29N3O4
  • MW: 423.50
  • Catalog: Bcl-2 Family
  • Density: 1.3±0.1 g/cm3
  • Boiling Point: 669.4±55.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 358.6±31.5 °C

(Arg6,D-Trp7.9,N-Me-Phe8)-Substance P (6-11)

Antagonist G is a potent vasopressin antagonist. Antagonist G is also a weak antagonist of GRP and Bradykinin. Antagonist G induces AP-1 transcription and sensitizes cells to chemotherapy[1][2].

  • CAS Number: 115150-59-9
  • MF: C49H66N12O6S
  • MW: 951.19000
  • Catalog: Apoptosis
  • Density: 1.35g/cm3
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Topoisomerase II inhibitor 15

Topoisomerase II inhibitor 15 (compound 2g) is a Topoisomerase II inhibitor. Topoisomerase II inhibitor 15 potently is an apoptotic inducer with greater selectivity against head and neck tumors[1].

  • CAS Number: 451516-79-3
  • MF: C15H11Cl2N5
  • MW: 332.19
  • Catalog: Apoptosis
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

SEL24-B489

SEL24-B489 (SEL24) is a potent, dual PIM and FLT3-ITD inhibitor with Kd of 2/2/3 nM for PIM1/2/3, Kd of 160/16 nM for FLT3-WT/FLT3-ITD, respectively; exhibits significantly broader on-target activity in AML cell lines (MV-4-11 GI50=20 nM) and primary AML blasts than selective FLT3-ITD or PIM inhibitors, decreases viability of AML cells with FLT3-TKD mutations associated with resistance to selective FLT3-ITD inhibitors; inhibits the growth of a broad panel of AML cell lines in xenograft models. Blood Cancer Phase 2 Clinical

  • CAS Number: 1616359-00-2
  • MF: C15H18Br2N4O2
  • MW: 446.143
  • Catalog: Apoptosis
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Isoalantolactone

Isoalantolactone is an apoptosis inducer, which also acts as an alkylating agent.

  • CAS Number: 470-17-7
  • MF: C15H20O2
  • MW: 232.318
  • Catalog: Apoptosis
  • Density: 1.1±0.1 g/cm3
  • Boiling Point: 364.9±42.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 152.7±25.3 °C

2-Hydroxychalcone

2-hydroxychalcone, a natural flavonoid, is a potent antioxidant, inhibiting lipid peroxidation. 2-Hydroxychalcone induces apoptosis by Bcl-2 downregulation. 2-Hydroxychalcone inhibits the activation of NF-kB[1][2][3].

  • CAS Number: 644-78-0
  • MF: C15H12O2
  • MW: 224.25500
  • Catalog: Apoptosis
  • Density: 1.191g/cm3
  • Boiling Point: 396.566ºC at 760 mmHg
  • Melting Point: 144-150ºC(lit.)
  • Flash Point: 169.35ºC

COTI-2

COTI-2 is a small molecule candidate anti-cancer drug which can convert mutant p53 to wild-type conformation.

  • CAS Number: 1039455-84-9
  • MF: C19H22N6S
  • MW: 366.483
  • Catalog: MDM-2/p53
  • Density: 1.3±0.1 g/cm3
  • Boiling Point: 574.1±60.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 301.0±32.9 °C

Z-Val-Glu-Ile-Asp-AFC

Z-VEID-AFC is a fluorescent substrate for caspase-6[1].

  • CAS Number: 219138-06-4
  • MF: C38H44F3N5O12
  • MW: 819.78
  • Catalog: Caspase
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Mefuparib hydrochloride(CVL218)

Mefuparib hydrochloride is a potent, highly selective, competitive PARP1/2 inhibitor with IC50 of 3.2/1.9 nM, respectively; displays >406-fold over other major nuclear PARPs including PARP3, TNKS1, TNKS2 and PARP6; reduces poly(ADP-ribose) formation, enhances γH2AX levels, induces G2/M arrest and subsequent apoptosis in homologous recombination repair (HR)-deficient cells; shows potent in vitro and in vivo proliferation and growth inhibition against HR-deficient cancer cells and synergistic sensitization of HR-proficient xenografts to the anticancer drug temozolomide.

  • CAS Number: 1449746-00-2
  • MF: C17H16ClFN2O2
  • MW: 334.775
  • Catalog: Apoptosis
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

NSC 15364

NSC 15364 is an inhibitor of VDAC1 oligomerization and apoptosis[1].

  • CAS Number: 4550-72-5
  • MF: C13H14N4O
  • MW: 242.277
  • Catalog: Apoptosis
  • Density: 1.4±0.1 g/cm3
  • Boiling Point: 381.4±27.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 184.5±23.7 °C

Tamoxifen-d3

Tamoxifen-d3 is the deuterium labeled Tamoxifen[1]. Tamoxifen (ICI 47699) is an orally active, selective estrogen receptor modulator (SERM) which blocks estrogen action in breast cells and can activate estrogen activity in other cells, such as bone, liver, and uterine cells[2][3][4]. Tamoxifen is a potent Hsp90 activator and enhances the Hsp90 molecular chaperone ATPase activity. Tamoxifen also potent inhibits infectious EBOV Zaire and Marburg (MARV) with IC50 of 0.1 μM and 1.8 μM, respectively[6]. Tamoxifen activates autophagy and induces apoptosis[5]. Tamoxifen also can induce gene knockout of CreER(T2) transgenic mouse[7].

  • CAS Number: 508201-30-7
  • MF: C26H26D3NO
  • MW: 374.53
  • Catalog: Apoptosis
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

PI3K-IN-33

PI3K-IN-33 (Compound 6e) is a highly selective PI3K inhibitor with IC50 values of 11.73, 6.09 and 11.18 μM for PI3K-α、PI3K-β and PI3K-δ , respectively. PI3K-IN-33 arrests cell cycle at G2/M phase and induces apoptosis. PI3K-IN-33 can be used in leukemia research[1].

  • CAS Number: 2458163-92-1
  • MF: C23H21BrN6O2
  • MW: 493.36
  • Catalog: Apoptosis
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

tubulin polymerization inhibitor

Tubulin polymerization-IN-24 (compound HMBA) is a potent tubulin polymerization inhibitor. Tubulin polymerization-IN-24 inhibits MCF-7 cells proliferation. Tubulin polymerization-IN-24 induces apoptosis and cell cycle arrest at G2/M phase. Tubulin polymerization-IN-24 increase the GTP hydrolysis rate and inhibits microtubule assembly[1].

  • CAS Number: 564468-51-5
  • MF: C22H16O3
  • MW: 328.361
  • Catalog: Apoptosis
  • Density: 1.3±0.1 g/cm3
  • Boiling Point: 524.7±50.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 188.8±23.6 °C

Anticancer agent 127

Anticancer agent 127 (142D6) is an IAP inhibitor that covalently targets the BIR3 domains of XIAP, cIAP1, and cIAP2. Anticancer agent 127 targets the BIR3 domains of XIAP, cIAP1, and cIAP2 with IC50s of 12 nM, 14 nM, and 9 nM, respectively. Anticancer agent 127 has anticancer effects[1].

  • CAS Number: 2410953-19-2
  • MF: C26H37FN4O6S
  • MW: 552.66
  • Catalog: IAP
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

KWCN-41

KWCN-41 is a selective and efficient inhibitor of RIPK1 kinase with an IC50 value of 88 nM. KWCN-41 specifically inhibits cell necrosis but does not inhibit apoptosis. KWCN-41 also has anti-inflammatory effects[1].

  • CAS Number: 2913223-17-1
  • MF: C18H17N3O2
  • MW: 307.35
  • Catalog: RIP kinase
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

MX1013

MX1013 is a potent, irreversible dipeptide caspase inhibitor vith antiapoptotic activity. MX1013 inhibits recombinant human caspase 3 with an IC50 of 30 nM[1].

  • CAS Number: 582316-00-5
  • MF: C18H23FN2O6
  • MW: 382.38300
  • Catalog: Caspase
  • Density: 1.258g/cm3
  • Boiling Point: 639.809ºC at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 340.745ºC

AMG-176

AMG-176 is a potent, selective and orally bioavailable MCL-1 inhibitor, with a Ki of 0.13 nM.

  • CAS Number: 1883727-34-1
  • MF: C33H41ClN2O5S
  • MW: 613.21
  • Catalog: Bcl-2 Family
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

HDAC4-IN-1

HDAC4-IN-1 (compound 1a) is a class IIa HDACI inhibitor (IC50=0.077 μM). HDAC4-IN-1 can enhance Caspase-induced Apoptosis. HDAC4-IN-1 has anticancer activity. HDAC4-IN-1 can be used in the research of drug combination against cancer[1].

  • CAS Number: 1418293-39-6
  • MF: C16H19F3N4O2
  • MW: 356.34
  • Catalog: Apoptosis
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Actein

Actein is a triterpene glycoside isolated from the rhizomes of Cimicifuga foetida. Actein suppresses cell proliferation, induces autophagy and apoptosis through promoting ROS/JNK activation, and blunting AKT pathway in human bladder cancer. Actein has little toxicity in vivo[1][2].

  • CAS Number: 18642-44-9
  • MF: C37H56O11
  • MW: 676.834
  • Catalog: Apoptosis
  • Density: 1.4±0.1 g/cm3
  • Boiling Point: N/A
  • Melting Point: 246-250ºC
  • Flash Point: N/A

RG7112

RG7112 is the first clinical and orally available MDM-2/p53 inhibitor designed to occupy the p53-binding pocket of MDM2, with the Kd value of 11 nM.

  • CAS Number: 939981-39-2
  • MF: C38H48Cl2N4O4S
  • MW: 727.783
  • Catalog: MDM-2/p53
  • Density: 1.2±0.1 g/cm3
  • Boiling Point: 790.4±70.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 431.8±35.7 °C

Liperfluo

Liperfluo, a marker of ferroptosis, is a useful fluorescent probe for investigating the roles of lipid peroxidation in a variety of cell pathophysiologies. Liperfluo reduces lipid hydroperoxides to lipid alcohols and is used for imaging lipid hydroperoxides in living cells[1][2][3].

  • CAS Number: 1448846-35-2
  • MF: C51H41N2O8P
  • MW: 840.85
  • Catalog: Ferroptosis
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

AKOS-22

AKOS-22 is a potent mitochondrial protein VDAC1 (voltage-dependent anion channel 1) inhibitor (Kd=15.4 μM). AKOS-22 interacts with VDAC1 and inhibiting both VDAC1 oligomerization and apoptosis. AKOS-22 protects against mitochondrial dysfunction[1].

  • CAS Number: 878983-38-1
  • MF: C22H21ClF3N3O3
  • MW: 467.87
  • Catalog: Apoptosis
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

HA14-1

HA14-1 is a Bcl-2/Bcl-XL antagonist. HA14-1 binds the designated pocket on Bcl-2 with the IC50 of ≈9 μM in competing with the Bcl-2 binding of Flu-BakBH3, and inhibits its function.

  • CAS Number: 65673-63-4
  • MF: C17H17BrN2O5
  • MW: 409.231
  • Catalog: Bcl-2 Family
  • Density: 1.5±0.1 g/cm3
  • Boiling Point: 535.1±50.0 °C at 760 mmHg
  • Melting Point: 105ºC
  • Flash Point: 277.4±30.1 °C

Oleic Acid-13C

Oleic acid-13C (9-cis-Octadecenoic acid-13C) is the 13C labeled Oleic acid. Oleic acid (9-cis-Octadecenoic acid) is an abundant monounsaturated fatty acid[1]. Oleic acid is a Na+/K+ ATPase activator[2].

  • CAS Number: 82005-44-5
  • MF: C1713CH34O2
  • MW: 283.45400
  • Catalog: Apoptosis
  • Density: 0.893 g/mL at 25ºC
  • Boiling Point: 192-195ºC1.2 mm Hg(lit.)
  • Melting Point: 13.4ºC(lit.)
  • Flash Point: >230 °F(lit.)

Ascorbic acid

L-Ascorbic acid is an effective reducing agent and donor antioxidant.

  • CAS Number: 50-81-7
  • MF: C6H8O6
  • MW: 176.124
  • Catalog: Apoptosis
  • Density: 2.0±0.1 g/cm3
  • Boiling Point: 552.7±50.0 °C at 760 mmHg
  • Melting Point: 190-194 °C (dec.)
  • Flash Point: 238.2±23.6 °C

Navitoclax-piperazine

Navitoclax-piperazine (ABT-263-piperazine) is a B-cell lymphoma extra large (BCL-XL) inhibitor. Navitoclax-piperazine and a VHL ligand for the E3 ubiquitin ligase can be used in the synthesis of PROTAC DT2216 (HY-130604)[1].

  • CAS Number: 2143096-93-7
  • MF: C47H56ClF3N6O5S3
  • MW: 973.63
  • Catalog: Bcl-2 Family
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

NF-κB-IN-5

NF-κB-IN-5 (compound 4d) is an orally active and potent NF-κB inhibitor by interacting directly with NF-κB. NF-κB-IN-5 shows antitumor activity against human cancer cell lines (HCT116, U87-MG, HepG2, BGC823, PC9), with IC50 values of 5.35, 2.81, 2.83, 2.02 and 3.90 μM, respectively. NF-κB-IN-5 induces apoptosis in U87-MG tumor cell and cell cycle arrest in G0/G1 phase[1].

  • CAS Number: 2425675-52-9
  • MF: C23H27N3O4
  • MW: 409.48
  • Catalog: Apoptosis
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Apoptosis inducer 4

Apoptosis inducer 4 (Compound 12b) is an apoptosis inducer with anticancer activities[1].

  • CAS Number: 2408050-83-7
  • MF: C41H50O11S3
  • MW: 815.02
  • Catalog: Apoptosis
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

TVB-3166

TVB-3166 is an orally-available, reversible, and selective fatty acid synthase (FASN) inhibitor with IC50s of 42 nM and 81 nM for biochemical FASN and cellular palmitate synthesis, respectively. TVB-3166 induces apoptosis, and inhibits in-vivo xenograft tumor growth[1].

  • CAS Number: 1533438-83-3
  • MF: C24H24N4O
  • MW: 384.47
  • Catalog: Apoptosis
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A