The intact ARP2/3 complex is first purified from Acanthamoeba castellanii based on its affinity for the actin-binding protein profilin, and is shown to consist of a stable assembly of seven polypeptides. Two of the subunits are actin-related proteins of the ARP2 and ARP3 subfamilies, giving the complex its name. The ARP2/3 complex possesses little biochemical activity on its own. However, when engaged by nucleation-promoting factor (NPF) proteins, it is activated to initiate the formation of a new (daughter) filament that emerges from an existing (mother) filament in a y-branch configuration with a regular 70° branch angle. This coupling of nucleation and branching by the ARP2/3 complex is referred to as autocatalytic branching or dendritic nucleation, and is central to its functions in vivo. Polymerization of actin filaments directed by the Arp2/3 complex supports many types of cellular movements.


Anti-infection >
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Latrunculin A

Latrunculin A (LAT-A) is a toxin isolated from the red sea sponge Latrunculia magnifica, binds to actin monomers, inhibits polymerization of actin, with Kds of 0.1, 0.4, 4.7 μM and 0.19 μM for ATP-actin, ADP-Pi-actin, ADP-actin and G-actin, respectively[1][2].

  • CAS Number: 76343-93-6
  • MF: C22H31NO5S
  • MW: 421.550
  • Catalog: Arp2/3 Complex
  • Density: 1.2±0.1 g/cm3
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

UNII:S831Z48C5W

Benproperine phosphate is an orally active, potent actin-related protein 2/3 complex subunit 2 (ARPC2) inhibitor. Benproperine phosphate attenuates the actin polymerization rate of action polymerization nucleation by impairing Arp2/3 function. Benproperine phosphate has the potential for a cough suppressant and suppresses cancer cell migration and tumor metastasis[1].

  • CAS Number: 19428-14-9
  • MF: C21H30NO5P
  • MW: 407.440
  • Catalog: Arp2/3 Complex
  • Density: N/A
  • Boiling Point: 433ºC at 760mmHg
  • Melting Point: 148-153ºC
  • Flash Point: N/A

CK 666

CK-666 is a cell-permeable inhibitor of actin-related protein Arp2/3 complex, and binds to Arp2/3 complex, stabilizes the inactive state of the complex, blocking movement of the Arp2 and Arp3 subunits into the activated filament-like (short pitch) conformation[1].

  • CAS Number: 442633-00-3
  • MF: C18H17FN2O
  • MW: 296.33900
  • Catalog: Arp2/3 Complex
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

187-1, N-WASP inhibitor TFA

187-1, N-WASP inhibitor, a 14-aa cyclic peptide, is an allosteric neural Wiskott-Aldrich syndrome protein (N-WASP) inhibitor. 187-1, N-WASP inhibitor potently inhibits actin assembly induced by phosphatidylinositol 4,5-bisphosphate (PIP2) with an IC50 of 2 μM. 187-1, N-WASP inhibitor prevents the activation of Arp2/3 complex by N-WASP by stabilizing the autoinhibited state of the protein[1][2].

  • CAS Number: 380488-27-7
  • MF: C96H122N18O16
  • MW: 1784.11000
  • Catalog: Arp2/3 Complex
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

CK869

CK-869 is an Actin-Related Protein 2/3 (ARP2/3) complex inhibitor, with an IC50 of 7 μM.

  • CAS Number: 388592-44-7
  • MF: C17H16BrNO3S
  • MW: 394.28300
  • Catalog: Arp2/3 Complex
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

CK-636

CK-636 is a cell permeable inhibitor of Arp2/3 complex, that could inhibit actin polymerization, with IC50 values of 4 μM, 24 μM and 32 μM for human, fission yeast and bovine, respectively.

  • CAS Number: 442632-72-6
  • MF: C16H16N2OS
  • MW: 284.376
  • Catalog: Arp2/3 Complex
  • Density: 1.3±0.1 g/cm3
  • Boiling Point: 576.8±45.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 302.6±28.7 °C

EG-011

EG-011 is the first-in-class and potent Wiskott-Aldrich syndrome protein (WASP) activator. EG-011 activates the auto-inhibited form of WASP with strong actin polymerization. EG-011 has selective anti-tumor activity in lymphomas[1][2][3].

  • CAS Number: 2377113-41-0
  • MF: C28H26N4O4
  • MW: 482.53
  • Catalog: Arp2/3 Complex
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Jasplakinolide

Jasplakinolide is a potent actin polymerization inducer and stabilizes pre-existing actin filaments. Jasplakinolide binds to F-actin competitively with phalloidin with a Kd of 15 nM. Jasplakinolide, a naturally occurring cyclic peptide from the marine sponge, has both fungicidal and anti-cancer activity[1][2].

  • CAS Number: 102396-24-7
  • MF: C36H45BrN4O6
  • MW: 709.670
  • Catalog: Fungal
  • Density: 1.2±0.1 g/cm3
  • Boiling Point: 968.3±65.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 539.4±34.3 °C

Cytochalasin B

Cytochalasin B is a cell-permeable mycotoxin binding to the barbed end of actin filaments, disrupting the formation of actin polymers, with Kd value of 1.4-2.2 nM for F-actin.

  • CAS Number: 14930-96-2
  • MF: C29H37NO5
  • MW: 479.608
  • Catalog: Arp2/3 Complex
  • Density: 1.2±0.1 g/cm3
  • Boiling Point: 740.6±60.0 °C at 760 mmHg
  • Melting Point: 218-223ºC
  • Flash Point: 401.7±32.9 °C

Dihydrocytochalasin B

Dihydrocytochalasin B is a Cytokinesis inhibitor and changes the morphology of the cells, similar to that of cytochalasin B; does not inhibit glucose transport[1]. Dihydrocytochalasin B (H2CB) disrupts the actin structure and inhibits the ability of growth factors to stimulate DNA synthesis, reversibly blocks initiation of DNA synthesis, causes cell rounding and a loss of actin microfilament bundles[2]. Dihydrocytochalasin B is related to transcytoplasmic movement of calcium by inhibiting active calcium transport and causes a Ca+increase in the mucosal scrapings[3].

  • CAS Number: 39156-67-7
  • MF: C29H39NO5
  • MW: 481.62400
  • Catalog: Arp2/3 Complex
  • Density: 1.194g/cm3
  • Boiling Point: 727.474ºC at 760 mmHg
  • Melting Point: 203-205ºC
  • Flash Point: 393.762ºC

Lygosporin A

Cytochalasin D (Zygosporin A; NSC 209835) is a potent and cell-permeable inhibitor of actin polymerization derived from fungus, inhibits the G-actin–cofilin interaction by binding to G-actin. Cytochalasin D (Zygosporin A; NSC 209835) also inhibits the binding of cofilin to F-actin and decreases the rate of both actin polymerization and depolymerization in living cells[1][2][3].

  • CAS Number: 22144-77-0
  • MF: C30H37NO6
  • MW: 507.618
  • Catalog: Arp2/3 Complex
  • Density: 1.2±0.1 g/cm3
  • Boiling Point: 712.1±60.0 °C at 760 mmHg
  • Melting Point: 255-260ºC
  • Flash Point: 384.5±32.9 °C

Wiskostatin

Wiskostatin is a potent and selective inhibitor of neuronal Wiskott-Aldrich syndrome protein (N-WASP)-mediated actin polymerization. Wiskostatin causes a rapid, profound, and irreversible decrease in cellular ATP levels[1].

  • CAS Number: 253449-04-6
  • MF: C17H18Br2N2O
  • MW: 426.146
  • Catalog: Arp2/3 Complex
  • Density: 1.6±0.1 g/cm3
  • Boiling Point: 320.2±22.0 °C at 760 mmHg
  • Melting Point: 153-155ºC
  • Flash Point: 147.4±22.3 °C