MAPK families play an important role in complex cellular programs like proliferation, differentiation, development, transformation, and apoptosis. In mammalian cells, three MAPK families have been clearly characterized: namely classical MAPK (ERK), C-Jun N-terminal kinse/ stress-activated protein kinase (JNK/SAPK) and p38 kinase. Each MAPK-related cascade consists of no fewer than three enzymes that are activated in series: a MAPK kinase kinase (MAPKKK), a MAPK kinase (MAPKK) and a MAP kinase (MAPK).

The MAPK pathways are activated by diverse extracellular and intracellular stimuli including peptide growth factors, cytokines, hormones, and various cellular stressors. In the ERK signaling pathway, ERK1/2 is activated by MEK1/2, which is activated by Raf. Raf is activated by the Ras GTPase, whose activation is induced by RTKs such as the epidermal growth factor receptor. The JNK and p38 MAPK signaling pathways are activated by various types of cellular stress. The JNK pathway consists of JNK, a MAP2K such as MKK4 (SEK1) or MKK7, and a MAP3K such as ASK1, TAK1, MEKK1, or MLK3. In the p38 pathway, p38 is activated by MKK3 or MKK6, and these MAP2Ks are activated by the same MAP3Ks that function in the JNK pathway.

MAPK signaling pathways has been implicated in the development of many human diseases including Alzheimer's disease (AD), Parkinson's disease (PD), amyotrophic lateral sclerosis (ALS) and various types of cancers. Therefore, the development of small molecule drugs that selectively inhibit individual components of MAPK signaling pathways is a key therapeutic strategy for cancer and neurodegenerative disorders.

References:
[1] Zhang W, et al. Cell Research (2002) 12, 9-18.
[2] Kim EK, et al. Biochim Biophys Acta. 2010 Apr;1802(4):396-405.
[3] Kim EK, et al. Arch Toxicol. 2015 Jun;89(6):867-82.


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NCB-0846

NCB-0846 is an orally available TNIK inhibitor with an IC50 of 21 nM.

  • CAS Number: 1792999-26-8
  • MF: C21H21N5O2
  • MW: 375.432
  • Catalog: MAP4K
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

R1487 Hydrochloride

R1487 (Hydrochloride) is highly potent and highly selective inhibitors of p38α.target: p38α;R1487 (Hydrochloride) potently inhibits cytokine production in a variety of in vitro and in vivo models.[1]R1487 (Hydrochloride) inhibits production of TNFR by human monocytic cells (THP-1) and inhibits production of IL-1β in human whole blood (HWB) induced by LPS.[1]

  • CAS Number: 449808-64-4
  • MF: C19H19ClF2N4O3
  • MW: 424.829
  • Catalog: p38 MAPK
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Trametiglue

Trametiglue, a derivative of Trametinib (HY-10999), targets both KSR-MEK and RAF-MEK with unprecedented potency and selectivity via unique interfacial binding interactions[1].

  • CAS Number: 2666940-97-0
  • MF: C25H24FIN6O5S
  • MW: 666.46
  • Catalog: MEK
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Isoliquiritin apioside

Isoliquiritin apioside, a component isolated from Glycyrrhizae radix rhizome, significantly decreases PMA-induced increases in MMP9 activities and suppresses PMA-induced activation of MAPK and NF-κB. Isoliquiritin apioside auppresseses invasiveness and angiogenesis of cancer cells and endothelial cells[1].

  • CAS Number: 120926-46-7
  • MF: C26H30O13
  • MW: 550.509
  • Catalog: p38 MAPK
  • Density: 1.6±0.1 g/cm3
  • Boiling Point: 901.0±65.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 301.9±27.8 °C

JNJ-49095397

JNJ-49095397 (RV-568) is a specific narrow-spectrum kinase inhibitor that inhibits a selected set of kinases involved in COPD inflammation with IC50 of 5, 40 and 52 nM for p38α, p38γ and HCK, respectively; shows potent anti-inflammatory effects in monocytes and macrophages, demonstrates synergistic interaction in poly I:C-stimulated BEAS-2B cells and in the cigarette smoke model combined with corticosteroid. COPD Phase 2 Clinical

  • CAS Number: 1220626-82-3
  • MF: C34H36N6O4
  • MW: 592.687
  • Catalog: p38 MAPK
  • Density: 1.2±0.1 g/cm3
  • Boiling Point: 738.0±60.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 400.1±32.9 °C

Hu7691

Hu7691 is an orally active, selective Akt inhibitor with IC50s of 4.0 nM, 97.5 nM, 28 nM for Akt1, Akt2 and Akt3, respectively. Hu7691 inhibits tumor growth and enables decrease of cutaneous toxicity in mice[1].

  • CAS Number: 2360523-76-6
  • MF: C22H22ClF3N4O
  • MW: 450.88
  • Catalog: ROCK
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

CAY10756

ASK1-IN-1 is a CNS-penetrant ASK1 (apoptosis signal-regulating kinase 1) inhibitor, with good potency (cell IC50=138 nM; Biochemical IC50=21 nM)[1].

  • CAS Number: 2411382-24-4
  • MF: C19H19N9O2
  • MW: 405.41
  • Catalog: p38 MAPK
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

HPK1-IN-20

HPK1-IN-19 is a hematopoietic progenitor kinase 1 (HPK1) inhibitor extracted from patent WO2020235902A1 compound 106[1].

  • CAS Number: 2561490-78-4
  • MF: C26H28N6O2
  • MW: 456.54
  • Catalog: MAP4K
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

MCP110

MCP110 is an inhibitor of Ras/Raf-1 interaction. MCP110 blocks the interaction of Ras with Raf[1].

  • CAS Number: 521310-51-0
  • MF: C33H36N2O3
  • MW: 508.651
  • Catalog: Ras
  • Density: 1.1±0.1 g/cm3
  • Boiling Point: 686.3±55.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 368.8±31.5 °C

RLX-33

RLX-33 is a potent, selective and blood-brain barrier (BBB) penetrant relaxin family peptide 3 (RXFP3) antagonist, also blocks relaxin-3-induced ERK1/2 phosphorylation, with IC50 values of 2.36 μM for RXFP3, 7.82 and 13.86 μM for ERK1 and ERK2 phosphorylation, respectively. RLX-33 can block the stimulation of food intake induced by the RXFP3-selective agonist R3/I5 in rats. RLX-33 can be used for the research of metabolic syndrome[1].

  • CAS Number: 2784577-71-3
  • MF: C24H19ClN4O4
  • MW: 462.89
  • Catalog: ERK
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Enniatin B1

Enniatin B1 is a Fusarium mycotoxin. Enniatin B1 inhibits acyl-CoA: cholesterol acyltransferase (ACAT) activity with an IC50 of 73 μM in an enzyme assay using rat liver microsomes[1]. Enniatin B1 crosss the blood-brain barrier[2]. Enniatin B1 decreases the activation of ERK (p44/p42). Enniatin B1 inhibits moderately TNF-α-induced NF-κB activation[3].

  • CAS Number: 19914-20-6
  • MF: C34H59N3O9
  • MW: 653.847
  • Catalog: ERK
  • Density: 1.0±0.1 g/cm3
  • Boiling Point: 833.8±65.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 458.0±34.3 °C

HPK1-IN-24

HPK1-IN-24 (example 51) is a hematopoietic progenitor kinase 1 (HPK1) inhibitor with a Ki of 100 nM. HPK1-IN-24 has the potential for cancer research[1].

  • CAS Number: 2294966-07-5
  • MF: C19H14FN5
  • MW: 331.35
  • Catalog: MAP4K
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Mitogen-activated protein kinase 1

Mitogen-activated protein kinase 1 (MAPK1) can activate the downstream p38/NF-κB pathway. Mitogen-activated protein kinase 1 can regulate cellular processes in various sepsis-associated diseases. MAPK-catalyzed phosphorylation of substrate proteins functions as a switch to turn on or off the activity of the substrate protein[1].

  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

SL-327

SL327 inhibits MEK1 and MEK2, with IC50 values of 180 nM and 220 nM, respectively.

  • CAS Number: 305350-87-2
  • MF: C16H12F3N3S
  • MW: 335.347
  • Catalog: MEK
  • Density: 1.4±0.1 g/cm3
  • Boiling Point: 512.6±50.0 °C at 760 mmHg
  • Melting Point: 127-128.2ºC
  • Flash Point: 263.8±30.1 °C

VX-11e

VX-11e is a potent, selective, and orally bioavailable inhibitor of ERK with Ki < 2 nM.

  • CAS Number: 896720-20-0
  • MF: C24H20Cl2FN5O2
  • MW: 500.352
  • Catalog: ERK
  • Density: 1.4±0.1 g/cm3
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

H-Pro-Ser-OH

Prolylserine, a dipeptide, is an inhibitor of melanogenesis production in Mel-Ab cells. Prolylserine decreases expression of microphthalmia-associated transcription factor (MITF) and tyrosinase, induces phorphosylation of ERK, but not cAMP response element binding protein (CREB)[1].

  • CAS Number: 71835-80-8
  • MF: C8H14N2O4
  • MW: 202.21
  • Catalog: ERK
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

B-Raf IN 16

B-Raf IN 16 (Compound I) is a BRAF inhibitors that belongs a cyclic iminopyrimidine derivative. B-Raf IN 16 can be used for cancer research[1].

  • CAS Number: 2304746-24-3
  • MF: C20H19N5O3S
  • MW: 409.46
  • Catalog: Raf
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

GW284543

GW284543 (UNC10225170) is a selective MEK5 inhibitor[1].

  • CAS Number: 790186-68-4
  • MF: C23H20N2O3
  • MW: 372.42
  • Catalog: MEK
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Isobavachromene

4-Hydroxylonchocarpin is a chalcone compound from an extract of Psoralea corylifolia. 4-Hydroxylonchocarpin increases phosphorylation of p38 MAPK, JNK and ERK. 4-Hydroxylonchocarpin has diverse pharmacological activities, including antibacterial, antifungal, anticancer, antireverse transcriptase, antitubercular, antimalarial, anti-inflammatory and antioxidant activities[1].

  • CAS Number: 56083-03-5
  • MF: C20H18O4
  • MW: 322.354
  • Catalog: p38 MAPK
  • Density: 1.3±0.1 g/cm3
  • Boiling Point: 535.1±50.0 °C at 760 mmHg
  • Melting Point: 203-204℃
  • Flash Point: 193.7±23.6 °C

Tinlorafenib

Tinlorafenib (PF-07284890) (compound 10) is an orally active BRAF kinase inhibitor, with IC50s of 4.25 and 2.7 nM for BRAFV600E/V600K respectively. Tinlorafenib demonstrates CNS penetration and can be used in the research of BRAF-associated malignant and benign tumors of the CNS as well as extracranial malignancies[1].

  • CAS Number: 2573781-75-4
  • MF: C19H19ClF2N4O3S
  • MW: 456.89
  • Catalog: Raf
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

BSJ-04-122

BSJ-04-122 is a potent, selective, covalent dual MKK4/7 inhibitor with IC50 of 4/181 nM, displays excellent kinome selectivity;BSJ-04-122 covalently targets a conserved cysteine (Cys247 for MKK4, and Cys261 for MKK7) located before the DFG motif.SJ-04-122 exhibits potent cellular target engagement and induces robust target-specific downstream effects.In breast cancer cell lines, BSJ-04-122 potently inhibited JNK phosphorylation, BSJ-04-122 significantly decreased levels of T183/Y185 pJNK at 5 uM, resulting in complete inhibition at 10 uM in MDA-MB-231 cells.The combination of the dual MKK4/7 inhibitor BSJ-04-122 with a selective, covalent JNK inhibitor (JNK-IN-8) demonstrated an enhanced antiproliferative activity against triple-negative breast cancer cells.

  • CAS Number: 2513289-74-0
  • MF: C15H12ClN5O
  • MW: 313.745
  • Catalog: p38 MAPK
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

I-287

I-287 is a potent, selective, orally active inhibitor of PAR2, negative PAR2 allosteric modulator, inhibits PAR2-mediated activation of Gq and G12/13 but not Gi/o proteins (IC50=45-390 nM);I-287 is a negative allosteric modulator (NAM) and not an orthosteric competitive antagonist of hPAR2.I-287 inhibits PAR2-mediated activation of DAG/Ca2+/PKC and RhoA/SRF-RE, as well as FAK and ERK1/2 signaling pathways, shows no effect on PAR2-mediated recruitment of βarrestin2 and receptor internalization.I-287 inhibits PAR2-induced secretion of IL-8 cytokine in vitro and reduces Freund's adjuvant (CFA)-induced paw edema model in mice.

  • CAS Number: 2014368-93-3
  • MF: C30H30ClFN4O4
  • MW: 565.042
  • Catalog: Protease-Activated Receptor (PAR)
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

3-Deoxysappanone B

Deoxysappanone B (3-Deoxysappanone B) is a homoisoflavone compound isolated from Caesalpinia sappan L (Lignum Sappan). Deoxysappanone B has anti-neuroinflammatory and neuroprotective effects and inhibits the production of neuroinflammatory mediators by blocking the IκB kinase (IKK)-NF-κB and p38/ERK MAPK pathways. Deoxysappanone B can be used in disease studies of neuritis and inflammation-related neurological damage[1].

  • CAS Number: 113122-54-6
  • MF: C16H14O5
  • MW: 286.279
  • Catalog: p38 MAPK
  • Density: 1.5±0.1 g/cm3
  • Boiling Point: 587.4±50.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 225.0±23.6 °C

12-Hydroxyabieta-8(14),9(11),12-trien-7-one

Sugiol is an abietane diterpenoid, can be isolated from Calocedrus formosana bark. Sugiol has anti-inflammatory activity, could effectively reduce intracellular reactive oxygen species (ROS) production in lipopolysaccharide (LPS)-stimulated macrophages[1].

  • CAS Number: 511-05-7
  • MF: C20H28O2
  • MW: 300.435
  • Catalog: Interleukin Related
  • Density: 1.0±0.1 g/cm3
  • Boiling Point: 437.2±45.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 186.4±21.3 °C

NG25 trihydrochloride

NG25 trihydrochloride is a type II kinase inhibitor that inhibits MAP4K2 and TAK1. It also inhibits the Src family kinases Src and LYN and Abl family kinases as well as CSK, FER, and p38α. NG 25 prevents TNF-α-induced IKKα/β phosphorylation and IκB-α degradation in L929 cells. It inhibits secretion of IFN-α and IFN-β induced by CpG type B and CL097, respectively. NG 25 decreases cell viability of HCT116KRASWT, and to a greater degree of HCT116KRASG13D, colorectal cancer cells in a concentration-dependent manner. It also reduces tumor growth and increases the number of TUNEL-positive tumor cells in a CT26KRASG12D mouse orthotopic model of colorectal cancer.

  • CAS Number: 2108554-00-1
  • MF: C29H33Cl3F3N5O2
  • MW: 646.96
  • Catalog: MAP3K
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Carnosol

Carnosol is a potent Ribosomal S6 Kinase (RSK2) inhibitor that could be useful for treating gastric cancer, with an IC50 of ~5.5 μM.

  • CAS Number: 5957-80-2
  • MF: C20H26O4
  • MW: 330.418
  • Catalog: Ribosomal S6 Kinase (RSK)
  • Density: 1.3±0.1 g/cm3
  • Boiling Point: 524.8±50.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 187.0±23.6 °C

GNE 220

GNE-220 is a potent and selective inhibitor of MAP4K4 with an IC50 of 7 nM.

  • CAS Number: 1199590-75-4
  • MF: C25H26N8
  • MW: 438.53
  • Catalog: MAP4K
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

TAK-715

TAK-715 is a p38 MAPK inhibitor for p38α with IC50 of 7.1 nM, 28-fold more selective for p38α over p38β, no inhibition to p38γ/δ, JNK1, ERK1, IKKβ, MEKK1 or TAK1.IC50 value: 7.1 nM [1]Target: p38α MAPKin vitro: TAK 715 inhibits LPS-stimulated release of TNF-alpha from THP-1 with IC50 of 48 nM [1]. TAK 715 (10 μM) inhibits Wnt-3a-induced hDvl2 phosphorylation and the hDvl2 shift in U2OS-EFC cells [2]. The amide NH of TAK 715 is hydrogen bonded to the main-chain carbonyl of Met109 of p38 alpha. TAK 715 binds relatively high in the ATP pocket, occupying the hydrophobic back pocket, the adenine region and the front pocket of p38 as well as extending to most of the length of the Gly-rich loop [3].in vivo: TAK 715 (10 mg/kg, po) inhibits LPS-induced TNF-alpha production in mice with 87.6% inhibition. TAK 715 has a modest mouse bioavailability of 18.4% and a slightly improved rat bioavailability of 21.1%. TAK 715 has a modest mouse bioavailability of 18.4% and a slightly improved rat bioavailability of 21.1%. TAK 715 results in Cmax of 0.19 μg/mL and AUC(0-24 hours) of 1.16 μg·h/mL in rats. TAK 715 (30 mg/kg, po) significantly reduces the secondary paw volume with 25 % inhibition in a rat adjuvant-induced arthritis (AA) model [1].

  • CAS Number: 303162-79-0
  • MF: C24H21N3OS
  • MW: 399.508
  • Catalog: p38 MAPK
  • Density: 1.2±0.1 g/cm3
  • Boiling Point: 495.3±45.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 253.3±28.7 °C

SM 7368

SM-7368 is a potent NF-kB inhibitor that targets downstream of MAPK p38 activation[1]. SM-7368 inhibits TNF-α-induced MMP-9 upregulation. SM-7368 can be used for the research of chemotherapies targeting TNF-α-mediated tumor invasion and metastasis [2].

  • CAS Number: 380623-76-7
  • MF: C10H5ClN4O5S
  • MW: 328.68900
  • Catalog: p38 MAPK
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

TAS0612

TAS0612 is an orally novel inhibitor of RSK、AKT and S6K. TAS0612 has broad-spectrum activity against tumor models via inhibiting cell growth[1].

  • CAS Number: 2148902-58-1
  • MF: C27H34F3N9O2
  • MW: 573.61
  • Catalog: Ribosomal S6 Kinase (RSK)
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A