The TGF-β superfamily comprises TGF-βs, bone morphogenetic proteins (BMPs), activins and related proteins. These proteins were identified mainly through their roles in development; they regulate the establishment of the body plan and tissue differentiation through their effects on cell proliferation, differentiation and migration. There are eight vertebrate Smads: Smad1 to Smad8. Smad2 and Smad3 are activated through carboxy-terminal phosphorylation by the TGF-b and activin receptors TbRI and ActRIB, whereas Smad1, Smad5 and Smad8 are activated by ALK-1, ALK-2, BMP-RIA/ALK-3 and BMP-RIB/ALK-6 in response to BMP1–4 or other ligands.

TGF-β binds two receptor types, the TGF-β type I and type II receptors (TβRI and TβRII, respectively) to form the active signaling complex. The TβRII activates TβRI kinase activity by phosphorylating the TβRI, which then transmits the signal intracellularly by phosphorylating the Smad transcription factors. The Smads constitutively shuttle between the cytoplasm and nucleus, but signaling causes the Smads to accumulate predominantly in the nucleus where they bind DNA and other transcriptional machinery to regulate the expression of target genes. TGF-β also involves in the regulations of PI3K and MAPK signaling pathways.

Abnormalities of the TGF-beta receptors and SMADs have been detected in various tumors, including colorectal cancers and pancreatic cancers. In addition, TGF-β/BMP signaling is also involved in osteoblast differentiation, chondrocyte differentiation, skeletal development, cartilage formation, bone formation, bone homeostasis, and related human bone diseases caused by the disruption ofTGF-β/BMP signaling.

References:
[1] Derynck R, et al. Nature. 2003 Oct 9;425(6958):577-84.
[2] Clarke DC, et al. Trends Cell Biol. 2008 Sep;18(9):430-42.
[3] Wu M, et al. Bone Res. 2016 Apr 26;4:16009.


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Leucosceptoside A

Leucosceptoside A is a phenylethanoid glycoside with anti-hyperglycemic and anti-hypertensive activities. Leucosceptoside A shows inhibitory activity against α-glucosidase and PKCα (IC50 of 19.0 μM)[1][2][3].

  • CAS Number: 83529-62-8
  • MF: C30H38O15
  • MW: 638.61400
  • Catalog: PKC
  • Density: 1.545g/cm3
  • Boiling Point: 884.547ºC at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 283.993ºC

Protein Kinase C (661-671)

Protein Kinase C (661-671) is a fragment peptide of β1 subspecies of protein kinase C (PKC). PKC plays a role in cellular growth control and tumor promotion[1][2].

  • CAS Number: 149839-93-0
  • MF: C59H87N13O19
  • MW: 1282.40
  • Catalog: PKC
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Verosudil(AR-12286)

Verosudil (AR-12286) is a potent, selective Rho-kinase (ROCK) inhibitor with Kis of 2 and 2 nM for ROCK1 and ROCK2, respectively. AR-12286 lowers intraocular pressure (IOP) primarily by increasing aqueous humour outflow through the trabecular meshwork[1][2].

  • CAS Number: 1414854-42-4
  • MF: C17H17N3O2S
  • MW: 327.401
  • Catalog: ROCK
  • Density: 1.3±0.1 g/cm3
  • Boiling Point: 625.5±55.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 332.1±31.5 °C

GSK-3β inhibitor 13

GSK-3β inhibitor 13 (compound 47) is an orally active and potent GSK-3β inhibitor with blood-brain permeability. GSK-3β inhibitor 13 inhibits GSK-3β and GSK-3α with IC50s of 0.73 nM and 0.35 nM, respectively. GSK-3β inhibitor 13 significantly decreases the phosphorylation of tau (IC50=58 nM), which leads the formation of the neurofibrillary tangles associated with Alzheimer's disease[1].

  • CAS Number: 2227316-74-5
  • MF: C23H22N6O2
  • MW: 414.46
  • Catalog: PKC
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

SD-208

SD-208 is a selective TGF-βRI (ALK5) inhibitor with IC50 of 48 nM, and > 100-fold selectivity over TGF-βRII.

  • CAS Number: 627536-09-8
  • MF: C17H10ClFN6
  • MW: 352.753
  • Catalog: TGF-β Receptor
  • Density: 1.5±0.1 g/cm3
  • Boiling Point: 460.4±45.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 232.2±28.7 °C

SAR407899 (hydrochloride)

SAR407899 hydrochloride is a selective, potent and ATP-competitive ROCK inhibitor, with an IC50 of 135 nM for ROCK-2, and Kis of 36 nM and 41 nM for human and rat ROCK-2, respectively.

  • CAS Number: 923262-96-8
  • MF: C14H17ClN2O2
  • MW: 280.75000
  • Catalog: ROCK
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Protein Kinase C (660-673)

Protein Kinase C (660-673) (PKC βII (660-673)) is the PKC βII V5 peptide with RACK1-binding affinity[1].

  • CAS Number: 149839-94-1
  • MF: C74H115N17O23
  • MW: 1610.81
  • Catalog: PKC
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

ROCK-IN-9

ROCK-IN-9 (Compound T345) is a ROCK inhibitor. ROCK-IN-9 shows cytotoxicity in HepG2 cell, with an IC50 of 40.8 μM. ROCK-IN-9 has good pharmacokinetic properties in mice, and shows high in vivo exposure and oral bioavailability at lower doses[1].

  • CAS Number: 2643334-76-1
  • MF: C20H20FN5O2
  • MW: 381.40
  • Catalog: ROCK
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

LDN 214117

LDN-214117 is a potent and selective ALK2 inhibitor with IC50 of 22 nM; > 100 fold selectivity for ALK5; also inhibits BMP6(IC50=100 nM).IC50 value: 22 nM(ALK2) [1]Target: ALK2 inhibitorLDN-214117 is a highly BMP selective compound, significantly biased toward ALK2 and its cognate ligands including BMP6 and also demonstrates a high degree of kinome selectivity and lowcytotoxicity. LDN-214117 may be useful as highly selectiveprobes of BMP-mediated cellular physiology that may provide auseful complement to the dorsomorphin class of compounds. Furthermore, this class of BMP inhibitors offers a structurallydistinct template for the development of therapeutics for thetreatment of BMP signaling-mediated diseases such as FOP.

  • CAS Number: 1627503-67-6
  • MF: C25H29N3O3
  • MW: 419.516
  • Catalog: TGF-β Receptor
  • Density: 1.1±0.1 g/cm3
  • Boiling Point: 567.9±50.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 297.3±30.1 °C

A 77-01

A 77-01 is a potent inhibitor of TGF-β type I receptor superfamily activin-like kinase ALK5 with IC50 of 25 nM. IC50 Value: 25 nM [1]Target: ALK5A-77-01 is a close analogue of A-83-01(Axon 1421) and has a very similar biological profile of A-83-01. A-83-01 is found to decompose to A-77-01 under certain circumstances and A-77-01 is likely an active component or metabolite of its prodrug A-83-01.

  • CAS Number: 607737-87-1
  • MF: C18H14N4
  • MW: 286.33100
  • Catalog: TGF-β Receptor
  • Density: 1.254g/cm3
  • Boiling Point: 481.63ºC at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 256.391ºC

GSK269962 HCl

GSK269962A hydrochloride (GSK 269962 hydrochloride) is a potent ROCK inhibitor with IC50s of 1.6 and 4 nM for recombinant human ROCK1 and ROCK2 respectively. GSK269962A hydrochloride has anti-inflammatory and vasodilatory activities[1].

  • CAS Number: 2095432-71-4
  • MF: C29H31ClN8O5
  • MW: 607.06
  • Catalog: ROCK
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

SR-3677

SR-3677 is a potent and selective ROCK-II inhibitor with an IC50 of ~3 nM.

  • CAS Number: 1072959-67-1
  • MF: C22H24N4O4
  • MW: 408.450
  • Catalog: Autophagy
  • Density: 1.3±0.1 g/cm3
  • Boiling Point: 646.9±55.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 345.0±31.5 °C

Chroman 1

Chroman 1 is a highly potent ROCK2 inhibitor, with an IC50 of 1 nM.

  • CAS Number: 1273579-40-0
  • MF: C24H28N4O4
  • MW: 436.50400
  • Catalog: ROCK
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Cirevetmab

Cirevetmab (ZTS-00521426) is an immunoglobulin G2-kappa, anti-[Canis lupus familiaris TGFB1], caninized monoclonal antibody. Cirevetmab is an immunomodulator[1].

  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Lerdelimumab

Lerdelimumab (CAT-152) is an IgG4 human anti-TGF-β2 recombinant monoclonal antibody. Lerdelimumab can be used as an anti-scarring agent for glaucoma research[1][2].

  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

C8-Ceramide

C8-Ceramide (N-Octanoyl-D-erythro-sphingosine) is a cell-permeable analog of naturally occurring ceramides. C8-Ceramide has anti-proliferation properties and acts as a potent chemotherapeutic agent. C8-Ceramide stimulates dendritic cells to promote T cell responses upon virus infections. C8-Ceramide induces slight activation of protein kinase (PKC) in vitro[1][2][3][4].

  • CAS Number: 74713-59-0
  • MF: C26H51NO3
  • MW: 425.688
  • Catalog: Apoptosis
  • Density: 0.9±0.1 g/cm3
  • Boiling Point: 595.5±50.0 °C at 760 mmHg
  • Melting Point: 68-70°C
  • Flash Point: 313.9±30.1 °C

Prostratin

Prostratin, a natural terpenoid compound, is a PKC activator, with a Ki of 12.5 nM and shows inhibitory effect on HIV-1.

  • CAS Number: 60857-08-1
  • MF: C22H30O6
  • MW: 390.470
  • Catalog: HIV
  • Density: 1.3±0.1 g/cm3
  • Boiling Point: 550.5±50.0 °C at 760 mmHg
  • Melting Point: 216-219℃
  • Flash Point: 188.7±23.6 °C

ROCK-IN-6

ROCK-IN-6 is a potent and selectiveROCK2 inhibitor with an IC50 of 2.19 nM. ROCK-IN-6 is extracted from patent WO2021164351 A1, example 7, has the potential for glaucoma and retinal diseases research[1].

  • CAS Number: 2489328-74-5
  • MF: C19H19N5O8S
  • MW: 477.45
  • Catalog: ROCK
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

(S)-Ro 32-0432 free base

(S)-Ro 32-0432 free base is a potent, selective, ATP-competitive and orally active PKC inhibitor. The IC50 values of (S)-Ro 32-0432 free base for PKCα, PKCβI, PKCβII, PKCγ and PKCε are 9.3 nM, 28 nM, 30 nM, 36.5 nM and 108.3 nM, respectively. (S)-Ro 32-0432 free base is also a selective G protein-coupled receptor kinase 5 (GRK5) inhibitor. (S)-Ro 32-0432 free base prevents T-cell activation and has the potential for chronic inflammatory and autoimmune diseases research[1][2].

  • CAS Number: 151342-35-7
  • MF: C28H28N4O2
  • MW: 452.55
  • Catalog: PKC
  • Density: N/A
  • Boiling Point: 711.9ºC at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 384.3ºC

Bisindolylmaleimide I HCl

Bisindolylmaleimide I (GF109203X) hydrochloride is a cell-permeable and reversible PKC inhibitor (IC50 of 20 nM, 17 nM, 16 nM, and 20 nM for PKCα, PKCβI, PKCβII, and PKCγ. Bisindolylmaleimide I hydrochloride is also a GSK-3 inhibitor[1][2][3].

  • CAS Number: 176504-36-2
  • MF: C25H25ClN4O2
  • MW: 448.94500
  • Catalog: PKC
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

(Ser25)-Protein Kinase C (19-31)

[Ser25] Protein Kinase C (19-31) is a substrate of protein kinase C (PKC) (Km: 0.3 μM). [Ser25] Protein Kinase C (19-31) is derived from the pseudo-substrate regulatory domain of PKCα (19-31) with a Serine at position 25 replacing the wild-type Alanine[1].

  • CAS Number: 136795-05-6
  • MF: C67H118N26O17
  • MW: 1559.818
  • Catalog: PKC
  • Density: 1.5±0.1 g/cm3
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Ro 32-0432 hydrochloride

Ro 32-0432 hydrochloride is a potent, selective, ATP-competitive and orally active PKC inhibitor. The IC50 values of Ro 32-0432 hydrochloride for PKCα, PKCβI, PKCβII, PKCγ and PKCε are 9.3 nM, 28 nM, 30 nM, 36.5 nM and 108.3 nM, respectively. Ro 32-0432 hydrochloride is also a selective G protein-coupled receptor kinase 5 (GRK5) inhibitor. Ro 32-0432 hydrochloride prevents T-cell activation and has the potential for chronic inflammatory and autoimmune diseases research[1][2].

  • CAS Number: 1781828-85-0
  • MF: C28H29ClN4O2
  • MW: 489.01
  • Catalog: PKC
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Pirfenidone

Pirfenidone is a drug used for the treatment of idiopathic pulmonary fibrosis. It inhibits FGFR, EGFR, PDGFR, TGF-β, thereby slowing tumor cell proliferation.

  • CAS Number: 53179-13-8
  • MF: C12H11NO
  • MW: 185.222
  • Catalog: TGF-beta/Smad
  • Density: 1.1±0.1 g/cm3
  • Boiling Point: 329.1±15.0 °C at 760 mmHg
  • Melting Point: 96-97ºC
  • Flash Point: 152.7±11.6 °C

D,L-erythro-Dihydrosphingosine

DL-erythro-Dihydrosphingosine is a potent inhibitor of PKC and phospholipase A2 (PLA2)[1][2].

  • CAS Number: 3102-56-5
  • MF: C18H39NO2
  • MW: 301.50800
  • Catalog: PKC
  • Density: 0.927g/cm3
  • Boiling Point: 446.2ºC at 760 mmHg
  • Melting Point: 70-72ºC
  • Flash Point: 223.7ºC

Go 6983

Go 6983 is a pan-PKC inhibitor against for PKCα, PKCβ, PKCγ, PKCδ and PKCζ with IC50 of 7 nM, 7 nM, 6 nM, 10 nM and 60 nM, respectively.

  • CAS Number: 133053-19-7
  • MF: C26H26N4O3
  • MW: 442.510
  • Catalog: PKC
  • Density: 1.3±0.1 g/cm3
  • Boiling Point: 709.0±60.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 382.6±32.9 °C

CGP60474

CGP60474 is a potent VEGFR-2 inhibitor, with an IC50 of 84 nM, and also an ATP-competitive PKC inhibitor.

  • CAS Number: 164658-13-3
  • MF: C18H18ClN5O
  • MW: 355.82100
  • Catalog: PKC
  • Density: 1.356g/cm3
  • Boiling Point: 630.6ºC at 760mmHg
  • Melting Point: N/A
  • Flash Point: 335.2ºC

K252C

K-252c, a staurosporine analog isolated from Nocardiopsis sp., is a cell-permeable PKC inhibitor, with an IC50 of 2.45 µM. K-252c induces apoptosis in human chronic myelogenous leukemia cancer cells. K-252c also inhibits β-lactamase, chymotrypsin, and malate dehydrogenase[1][2][3].

  • CAS Number: 85753-43-1
  • MF: C20H13N3O
  • MW: 311.33700
  • Catalog: Bacterial
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

6,7,4'-Trihydroxyisoflavone

Desmethylglycitein (4',6,7-Trihydroxyisoflavone), a metabolite of daidzein, sourced from Glycine max with antioxidant, and anti-cancer activities.Desmethylglycitein binds directly to CDK1 and CDK2 in vivo, resulting in the suppresses CDK1 and CDK2 activity[1]. Desmethylglycitein is a direct inhibitor of protein kinase C (PKC)α, against solar UV (sUV)-induced matrix matrix metalloproteinase 1 (MMP1)[2]. Desmethylglycitein binds to PI3K in an ATP competitive manner in the cytosol, where it inhibits the activity of PI3K and downstream signaling cascades, leading to the suppression of adipogenesis in 3T3-L1 preadipocytes[3].

  • CAS Number: 17817-31-1
  • MF: C15H10O5
  • MW: 270.23700
  • Catalog: CDK
  • Density: 1.548g/cm3
  • Boiling Point: 587.1ºC at 760mmHg
  • Melting Point: N/A
  • Flash Point: 229.7ºC

H-Arg-Gly-Tyr-Ala-Leu-Gly-OH

H-Arg-Gly-Tyr-Ala-Leu-Gly-OH is a competitive and CAMP dependent protein kinase inhibitor[1].

  • CAS Number: 59587-24-5
  • MF: C28H45N9O8
  • MW: 635.71200
  • Catalog: PKC
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

ala-gly-asn-lys-val-ile-ser-pro-ser-glu-asp-arg-arg-gln-cys

Protein kinase C (alpha) peptide (TFA) is a peptide of PKC-α. PKC-α acts as a lipid-dependent ser/thr protein kinase, can modulate various cellular processes, including cell survival, proliferation, differentiation, migration, adhesion and so on[1].

  • CAS Number: 159939-84-1
  • MF: C66H114N24O24S
  • MW: 1659.82000
  • Catalog: PKC
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A