Serine proteases are enzymes that cleave peptide bonds in proteins, in which serine serves as the nucleophilic amino acidat the active site. They are found ubiquitously in both eukaryotes and prokaryotes. Serine proteases fall into two broad categories based on their structure: chymotrypsin-like or subtilisin-like. In humans, serine proteases are responsible for co-ordinating various physiological functions, including digestion, immune response, blood coagulation and reproduction. Threnonine proteases are a family of proteolytic enzymes harbouring a threonine (Thr) residue within the active site. The prototype members of this class of enzymes are the catalytic subunits of the proteasome, however the acyltransferases convergently evolved the same active site geometry andmechanism.


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ANTHRANILYL-ASP-PHE-ARG-LEU-PHE-ALA-PHE&

Fluorescent Substrate for Subtillsin can be used to detect the enzyme activity of subtillsin. Subtilisin is a bacterial serine protease[1][2].

  • CAS Number: 147471-56-5
  • MF: C66H80N14O18
  • MW: 1357.424
  • Catalog: Ser/Thr Protease
  • Density: 1.4±0.1 g/cm3
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Benzenecarboximidamide hydrochloride hydrate

Benzenecarboximidamide, hydrochloride, hydrate (1:1:x) (Benzamidine hydrochloride hydrate) is a reversible competitive trypsin-like serine proteases inhibitor with Kis of 20, 21, 97, 110, 320 and 750 μM against Tryptase, Trypsin, uPA, Factor Xa, Thrombin and tPA, respectively[1][2].

  • CAS Number: 206752-36-5
  • MF: C7H8N2.ClH.xH2O
  • MW: 174.628
  • Catalog: Ser/Thr Protease
  • Density: N/A
  • Boiling Point: 208.5ºC at 760mmHg
  • Melting Point: 86-88 °C(lit.)
  • Flash Point: 79.9ºC

Rennin

Rennin, also known as Chymosin, is a pepsin-related proteolytic enzyme synthesized by cells in the stomach of certain animals that efficiently converts liquid milk into a semi-solid, allowing it to remain in the stomach for longer. The natural substrate of Rennin is K-casein, which is specifically cleaved at the peptide bond between amino acid residues 105 and 106, phenylalanine and methionine, and is widely used in cheese production[1].

  • CAS Number: 9001-98-3
  • MF: C7H6O4
  • MW: 154.120
  • Catalog: Ser/Thr Protease
  • Density: 1.5±0.1 g/cm3
  • Boiling Point: 513.7±50.0 °C at 760 mmHg
  • Melting Point: 225-227℃
  • Flash Point: 226.8±23.6 °C

Ecallantide

Ecallantide (DX-88) is a specific recombinant plasma kallikrein inhibitor. Ecallantide inhibits the production of bradykinin. Ecallantide can be used to prevent acute attacks of angioedema[1].

  • CAS Number: 460738-38-9
  • MF: C305H441N88O91S8
  • MW:
  • Catalog: Ser/Thr Protease
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Bezeparsen

Bezeparsen is a PCSK9 synthesis inhibitor[1].

  • CAS Number: 2639640-84-7
  • MF: C238H335N67O113P16S15
  • MW: 6919.13
  • Catalog: Ser/Thr Protease
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Ono 3403

Ono 3403 is a synthetic serine protease inhibitor. Ono 3403 inhibits lipopolysaccharide-induced tumor necrosis factor-alpha and nitric oxide production. ONO-3403 also has an antitumor effect on malignant tumors.

  • CAS Number: 181586-07-2
  • MF: C26H31N3O8S
  • MW: 545.6
  • Catalog: Apoptosis
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Patamostat

Patamostat (E-3123) is a potent protease inhibitor. Patamostat potently inhibits trypsin, plasmin and thrombin with IC50s of 39 nM, 950 nM and 1.9 μM, respectively. E-3123 may possess suppressing effects on pathogenesis and development of acute pancreatitis[1][2].

  • CAS Number: 114568-26-2
  • MF: C20H20N4O4S
  • MW: 412.46200
  • Catalog: Ser/Thr Protease
  • Density: 1.41g/cm3
  • Boiling Point: 701.5ºC at 760mmHg
  • Melting Point: N/A
  • Flash Point: 378.1ºC

PCSK9-IN-13

PCSK9-IN-13(compound 3f) is a potent PCSK9 inhibitor, which can antagonize low-density lipoprotein (LDL) receptor binding by binding to PCSK9, with an IC50 of 537 nM[1].

  • CAS Number: 2244129-23-3
  • MF: C30H33N5O2
  • MW: 495.62
  • Catalog: Ser/Thr Protease
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Tilpisertib fosmecarbil

Tilpisertib fosmecarbil is a potent serine/threonine kinase inhibitor. Tilpisertib fosmecarbil has anti-inflammatory activity[1].

  • CAS Number: 2567459-64-5
  • MF: C35H36ClN8O7P
  • MW: 747.14
  • Catalog: Ser/Thr Protease
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

α-lytic endopeptidase

From the myxobacterium Lysobacter enzymogenes. In peptidase family S1 (trypsin family) Reaction: Preferential cleavage: Ala┼, Val┼ in bacterial cell walls, elastin and other proteins

  • CAS Number: 37288-76-9
  • MF:
  • MW:
  • Catalog: Ser/Thr Protease
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

PKR-IN-C51

PKR-IN-C51(compound 51) is an ATP-competitive double-stranded RNA-activated protein kinase (PKR) inhibitor with an IC50 of 9 μM. PKR-IN-C51 inhibits intracellular PKR activation in a dose-dependent manner in primary mouse macrophages[1].

  • CAS Number: 1314594-23-4
  • MF: C23H21N5
  • MW: 367.45
  • Catalog: Ser/Thr Protease
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

3,4-DIHYDROISOQUINOLINE-2(1H)-CARBOXIMIDAMIDE

Debrisoquin (Isocaramidine) is a TMPRSS2 inhibitors that inhibits SARS-CoV-2 entry into human lung cell line by a TMPRSS2-depedent manner, with an IC50 of 22μM. Debrisoquin can be used for antiviral research[1].

  • CAS Number: 1131-64-2
  • MF: C10H13N3
  • MW: 175.23
  • Catalog: SARS-CoV
  • Density: 1.24g/cm3
  • Boiling Point: 309.8ºC at 760mmHg
  • Melting Point: N/A
  • Flash Point: 141.1ºC

Z-Val-Gly-Arg-pNA

Z-Val-Gly-Arg-pNA is a chromogenic substrate for urokinase. Z-Val-Gly-Arg-pNA can be used for determination of urokinase activity[1].

  • CAS Number: 78333-16-1
  • MF: C27H36N8O7
  • MW: 584.624
  • Catalog: Ser/Thr Protease
  • Density: 1.4±0.1 g/cm3
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Tosyl phenylalanyl chloromethyl ketone

TPCK (L-1-Tosylamido-2-phenylethyl chloromethyl ketone; L-TPCK) is a potent serine protease inhibitor that reacts with the retinoblastoma protein (RB)-binding core of HPV-18 E7 protein and abolish its RB-binding capability. TPCK is able to modify E7 protein in live keratinocytes following its addition to the culture medium[1].

  • CAS Number: 402-71-1
  • MF: C17H18ClNO3S
  • MW: 351.848
  • Catalog: Microtubule/Tubulin
  • Density: 1.3±0.1 g/cm3
  • Boiling Point: 509.9±60.0 °C at 760 mmHg
  • Melting Point: 106-108ºC(lit.)
  • Flash Point: 262.2±32.9 °C

UK-371804

UK-371804 is a urokinase-type plasminogen activator (uPA) inhibitor with a Ki of 10 nM.

  • CAS Number: 256477-09-5
  • MF: C14H16ClN5O4S
  • MW: 385.823
  • Catalog: Ser/Thr Protease
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

cyclotheonamide A

Cyclotheonamide A is a serine protease inhibitor (cyclic polypeptide), which can be obtained from marine sponges of the genus Theonella. Cyclotheonamide A shows potent inhibitory activity against trypsin (Ki=0.023 µM) and streptokinase (Ki=0.035 µM) and moderate inhibitory activity against human α-thrombin (Ki=0.18 µM). Cyclotheonamide A can be used in study of antithrombotic[1].

  • CAS Number: 129033-04-1
  • MF: C36H45N9O8
  • MW: 731.79800
  • Catalog: Ser/Thr Protease
  • Density: 1.44g/cm3
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

PF-06815345

PF-06815345 (PF 6815345) is a potent prodrug PCSK9 modulator that inhibits PCSK9 secretion in vitro translation assay; PF-06815345is identified after optimization of the prodrug moiety to maximize intestinal stability achieving liver targeting of the active compound after prodrug turnover by carboxylesterase (CES1). Hypercholesterolemia Phase 1 Discontinued

  • CAS Number: 1900686-46-5
  • MF: C27H29ClFN9O4
  • MW: 598.029
  • Catalog: Ser/Thr Protease
  • Density: 1.5±0.1 g/cm3
  • Boiling Point: 775.4±70.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 422.7±35.7 °C

HMB-Val-Ser-Leu-VE

HMB-Val-Ser-Leu-VE is a prototype vinyl ester inhibitor. HMB-Val-Ser-Leu-VE is against trypsin-like (T-L) proteasome activity with an IC50 of 0.033 μM[1].

  • CAS Number: 862891-04-1
  • MF: C26H39N3O7
  • MW: 505.60
  • Catalog: Ser/Thr Protease
  • Density: 1.2±0.1 g/cm3
  • Boiling Point: 754.1±60.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 409.8±32.9 °C

WX 671

Upamostat is a serine protease inhibitor. Upamostat is the orally available prodrug of the WX-UK1, which is a urokinase plasminogen activator (uPA) inhibitor.

  • CAS Number: 590368-25-5
  • MF: C32H47N5O6S
  • MW: 629.81000
  • Catalog: Ser/Thr Protease
  • Density: 1.25
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

3,4-Dichloro-isocoumarin

3,4-Dichloroisocoumarin is a potent serine-protease inhibitor that inhibits chymotrypsin-like activity[1].

  • CAS Number: 51050-59-0
  • MF: C9H4Cl2O2
  • MW: 215.03300
  • Catalog: Ser/Thr Protease
  • Density: 1.53 g/cm3
  • Boiling Point: 304.7ºC at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 136.6ºC

Irucalantide

Irucalantide is kallikrein inhibitor[1].

  • CAS Number: 1631160-47-8
  • MF: C76H106N20O18S3
  • MW: 1683.97
  • Catalog: Ser/Thr Protease
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Inclisiran

Inclisiran (ALN-PCSsc) is a double-stranded small interfering RNA (siRNA) molecule that inhibits the transcription of PCSK-9. Inclisiran can be used for hyperlipidemia and cardiovascular disease (CVD) research[1].

  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Berotralstat dihydrochloride

Berotralstat dihydrochloride is a low toxicity, effective, highly specific, second-generation, synthetic and orally active plasma kallikrein inhibitor used for the research of hereditary angioedema (HAE) attacks. Berotralstat dihydrochloride works by blocking the enzymatic activity of plasma kallikrein in releasing bradykinin, the major biologic peptide that promotes swelling and pain associated with attacks of HAE[1][2].

  • CAS Number: 1809010-52-3
  • MF: C30H28Cl2F4N6O
  • MW: 635.48
  • Catalog: Ser/Thr Protease
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Alirocumab

Alirocumab (REGN 727) is a human monoclonal antibody inhibiting proprotein convertase subtilisin/kexin type 9 (PCSK9). Alirocumab specifically binds PCSK9, a down regulator of liver low-density lipoprotein (LDL)-receptors, thereby increasing the ability of the liver to bind LDL-cholesterol (LDL-C) and reducing levels of LDL-C in blood. Alirocumab can be used for the research of hypercholesterolemia[1].

  • CAS Number: 1245916-14-6
  • MF: C6472H9996N1736O2032S42
  • MW:
  • Catalog: Ser/Thr Protease
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Benzimidamide hydrochloride

Benzamidine hydrochloride is an reversible competitive inhibitor of trypsin-like serine proteases, with Kis of 97 µM, 21 µM, 20 µM and 110 µM for uPA, trypsin, tryptase and factor Xa, respectively[1].

  • CAS Number: 1670-14-0
  • MF: C7H9ClN2
  • MW: 156.613
  • Catalog: Ser/Thr Protease
  • Density: 1.09 g/cm3
  • Boiling Point: 332.9ºC at 760 mmHg
  • Melting Point: 86-88 °C(lit.)
  • Flash Point: 155.1ºC

UK122

UK122 is a potent and selective urokinase-type plasminogen activator (uPA) inhibitor with an IC50 of 0.2 μM. UK122 shows no or little inhibition of tissue-type PA (tPA), plasmin, thrombin, and trypsin (all IC50>100 μM). UK122, 4-oxazolidinone analogue, is an anticancer agent and inhibits cancer cell migration and invasion[1].

  • CAS Number: 940290-58-4
  • MF: C17H13N3O2
  • MW: 291.30
  • Catalog: Ser/Thr Protease
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Urokinase

Urokinase peptidolytic (Urokinase-type plasminogen activator) is a serine protease, an inactive form (zymogen) of the serine protease plasminogen. Activation of plasmin triggers a proteolytic cascade reaction, which in turn participates in thrombolysis or extracellular matrix degradation, implicated in vascular disease and cancer-related research[1].

  • CAS Number: 9039-53-6
  • MF: C21H25BrN2O3
  • MW:
  • Catalog: Ser/Thr Protease
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Patomostat mesilate

Patamostat (E-3123) mesylate is a potent protease inhibitor. Patamostat mesylate potently inhibits trypsin, plasmin and thrombin with IC50s of 39 nM, 950 nM and 1.9 μM, respectively. Patamostat mesylate may possess suppressing effects on pathogenesis and development of acute pancreatitis[1][2].

  • CAS Number: 114568-32-0
  • MF: C21H24N4O7S2
  • MW: 508.56800
  • Catalog: Ser/Thr Protease
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Galegenimab

Galegenimab (FHTR 2163; RG 6147; RO 7171009), an anti-High-temperature requirement A1 (HTRA1) antibody fragment. Galegenimab can be used for age-related macular degeneration (AMD) research[1].

  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Pep 2-8

Pep2-8 is a proprotein convertase subtilisin/kexin type 9 (PCSK9) inhibitor, with an IC50 of 0.8 μM for PCSK9 binding to LDL receptor.

  • CAS Number: 1541011-97-5
  • MF: C83H110N16O24
  • MW: 1715.85
  • Catalog: Ser/Thr Protease
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A