Metabolic pathways are enzyme-mediated biochemical reactions that lead to biosynthesis (anabolism) or breakdown (catabolism) of natural product small molecules within a cell or tissue. In each pathway, enzymes catalyze the conversion of substrates into structurally similar products. Metabolic processes typically transform small molecules, but also include macromolecular processes such as DNA repair and replication, and protein synthesis and degradation. Metabolism maintains the living state of the cells and the organism.

Proteases are used throughout an organism for various metabolic processes. Proteases control a great variety of physiological processes that are critical for life, including the immune response, cell cycle, cell death, wound healing, food digestion, and protein and organelle recycling. On the basis of the type of the key amino acid in the active site of the protease and the mechanism of peptide bond cleavage, proteases can be classified into six groups: cysteine, serine, threonine, glutamic acid, aspartate proteases, as well as matrix metalloproteases. Proteases can not only activate proteins such as cytokines, or inactivate them such as numerous repair proteins during apoptosis, but also expose cryptic sites, such as occurs with β-secretase during amyloid precursor protein processing, shed various transmembrane proteins such as occurs with metalloproteases and cysteine proteases, or convert receptor agonists into antagonists and vice versa such as chemokine conversions carried out by metalloproteases, dipeptidyl peptidase IV and some cathepsins. In addition to the catalytic domains, a great number of proteases contain numerous additional domains or modules that substantially increase the complexity of their functions.

Imbalances in metabolic activities have been found to be critical in a number of pathologies, such as cardiovascular diseases, inflammation, cancer, and neurodegenerative diseases.

References:
[1] Turk B, et al. EMBO J. 2012 Apr 4;31(7):1630-43.
[2] Eatemadi A, et al. Biomed Pharmacother. 2017 Feb;86:221-231.


Anti-infection >
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15-PGDH 5 alpha Reductase 5-Lipoxygenase Acetyl-CoA Carboxylase Acyltransferase Adenosine Deaminase Adenosine Kinase Aldehyde Dehydrogenase (ALDH) Aldose Reductase Aminopeptidase Angiotensin-converting Enzyme (ACE) ATGL ATP Citrate Lyase Carbonic Anhydrase Carboxypeptidase Cathepsin CETP COMT Cytochrome P450 Dipeptidyl Peptidase Dopamine β-hydroxylase E1/E2/E3 Enzyme Elastase Enolase FAAH FABP Factor Xa Farnesyl Transferase Fatty Acid Synthase (FAS) FXR Glucokinase GSNOR Gutathione S-transferase HCV Protease Hexokinase HIF/HIF Prolyl-Hydroxylase HIV Integrase HIV Protease HMG-CoA Reductase (HMGCR) HSP Indoleamine 2,3-Dioxygenase (IDO) Isocitrate Dehydrogenase (IDH) Lactate Dehydrogenase LXR MAGL Mineralocorticoid Receptor Mitochondrial Metabolism MMP Nampt NEDD8-activating Enzyme Neprilysin PAI-1 PDHK PGC-1α Phosphatase Phosphodiesterase (PDE) Phospholipase Procollagen C Proteinase Proteasome Pyruvate Kinase RAR/RXR Renin ROR Ser/Thr Protease SGK Stearoyl-CoA Desaturase (SCD) Thrombin Tryptophan Hydroxylase Tyrosinase Xanthine Oxidase
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Enniatin B1

Enniatin B1 is a Fusarium mycotoxin. Enniatin B1 inhibits acyl-CoA: cholesterol acyltransferase (ACAT) activity with an IC50 of 73 μM in an enzyme assay using rat liver microsomes[1]. Enniatin B1 crosss the blood-brain barrier[2]. Enniatin B1 decreases the activation of ERK (p44/p42). Enniatin B1 inhibits moderately TNF-α-induced NF-κB activation[3].

  • CAS Number: 19914-20-6
  • MF: C34H59N3O9
  • MW: 653.847
  • Catalog: ERK
  • Density: 1.0±0.1 g/cm3
  • Boiling Point: 833.8±65.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 458.0±34.3 °C

Bortezomib (PS-341)

Bortezomib (PS-341) is a potent 20S proteasome inhibitor with a Ki of 0.6 nM.

  • CAS Number: 179324-69-7
  • MF: C19H25BN4O4
  • MW: 384.237
  • Catalog: Autophagy
  • Density: 1.2±0.1 g/cm3
  • Boiling Point: N/A
  • Melting Point: 122-124°C
  • Flash Point: N/A

Celastrol

Tripterin (Celastrol) is a proteasome inhibitor which potently and preferentially inhibits the chymotrypsin-like activity of a purified 20S proteasome with IC50 of 2.5 μM.

  • CAS Number: 34157-83-0
  • MF: C29H38O4
  • MW: 450.610
  • Catalog: Autophagy
  • Density: 1.2±0.1 g/cm3
  • Boiling Point: 645.7±55.0 °C at 760 mmHg
  • Melting Point: 185-200ºC
  • Flash Point: 358.3±28.0 °C

Rennin

Rennin, also known as Chymosin, is a pepsin-related proteolytic enzyme synthesized by cells in the stomach of certain animals that efficiently converts liquid milk into a semi-solid, allowing it to remain in the stomach for longer. The natural substrate of Rennin is K-casein, which is specifically cleaved at the peptide bond between amino acid residues 105 and 106, phenylalanine and methionine, and is widely used in cheese production[1].

  • CAS Number: 9001-98-3
  • MF: C7H6O4
  • MW: 154.120
  • Catalog: Ser/Thr Protease
  • Density: 1.5±0.1 g/cm3
  • Boiling Point: 513.7±50.0 °C at 760 mmHg
  • Melting Point: 225-227℃
  • Flash Point: 226.8±23.6 °C

saxagliptin

Saxagliptin(BMS477118) is a selective and reversible DPP4 inhibitor with IC50 of 26 nM and Ki of 1.3 nM.IC50 value: 26 nM [1]Target: DPP4in vitro: Saxagliptin has an inhibition constant Ki of 1.3 nM for DPP4 inhibition, which is 10-fold more potent than either vildagliptin or sitagliptin (another two DPP4 inhibitors) with Ki of 13 and 18 nM. In addition, Saxagliptin demonstrates greater specificity for DPP4 than for either the DPP8 or DPP9 enzymes (400- and 75- fold, respectively). The active metablite of saxagliptin is two-fold less potent than the parent. Both Saxagliptin and its metabolite are highly selective (>4000-fold) for the prevention of DPP4 compared with a range of other proteases (selectivity of sitagliptin and vildagliptin for DPP4 is >2600 and <250-fold, respectively, compared with DPP8 and DPP9) [2]. Saxagliptin reduces the degradation of the incretin hormone glucagon-like peptide-1, thereby enhancing its actions, and is associated with improved β-cell function and suppression of glucagon secretion.in vivo: Saxagliptin is highly effective at eliciting marked dose-dependent enhancements in glucose clearance in the dose range 0.13-1.3 mg/kg in ob/ob mice relative to controls. Saxagliptin dose-dependently elevate plasma insulin significantly at 15 min post-oGTT, with concomitant improvement in the glucose clearance curves at 60 min post-oGTT [4].

  • CAS Number: 361442-04-8
  • MF: C18H25N3O2
  • MW: 315.410
  • Catalog: Dipeptidyl Peptidase
  • Density: 1.35
  • Boiling Point: 548.7±35.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 285.6±25.9 °C

SB-435495 hydrochloride

SB-435495 hydrochloride is a potent, selective, reversible, non-covalent and orally active Lp-PLA2 inhibitor with an IC50 of 0.06 nM[1][3].

  • CAS Number: 304694-41-5
  • MF: C38H41ClF4N6O2S
  • MW: 757.28
  • Catalog: Phospholipase
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

HFI-419

HFI-419 is an insulin-regulated aminopeptidase (IRAP) inhibitor. HFI-419 has inhibitory potency for IRAP with Ki value of 0.48 μM. HFI-419 can be used for the research of cognitive and memory impairments such as Alzheimer's disease, brain trauma, and stroke[1].

  • CAS Number: 1110650-72-0
  • MF: C19H18N2O5
  • MW: 354.36
  • Catalog: Aminopeptidase
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

hCAIX-IN-10

hCAIX-IN-10 (Compound 6i) is a selective carbonic anhydrase IX and XII inhibitor with Ki s of 61.5 and 586.8 nM for hCA IX and hCA XII, respectively.  hCA IX and hCA XII are transmembrane isoforms which have been characterized as biomarkers for several types of tumors. The hCA XII assists in maintenance of acid-base homoeostasis in normal as well as tumor cells[1].

  • CAS Number: 2497504-72-8
  • MF: C28H21N3O3S
  • MW: 479.55
  • Catalog: Carbonic Anhydrase
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Leupeptin hemisulfate salt

Leupeptin hemisulfate is a reversible, competitive serine/cysteine protease inhibitor.

  • CAS Number: 103476-89-7
  • MF: C20H38N6O4.1/2H2SO4
  • MW: 475.59
  • Catalog: Peptides
  • Density: 1.2±0.1 g/cm3
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Sorbinil

Sorbinil, is an Aldose reductase inhibitor (ARI).Sorbinil plays therapeutic role in treating diabetes and diabetic complications, decreases AR activity and inhibits polyol pathway, it to be found comparatively safer than other ARIs for human use[1].

  • CAS Number: 68367-52-2
  • MF: C11H9FN2O3
  • MW: 236.19900
  • Catalog: Aldose Reductase
  • Density: 1.52g/cm3
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

H-Ile-Pro-Pro-OH

H-Ile-Pro-Pro-OH, a milk-derived peptide[1], inhibits angiotensin-converting enzyme (ACE)[1] with an IC50 of 5 μM[2]. Antihypertensive tripeptides[1].

  • CAS Number: 26001-32-1
  • MF: C16H27N3O4
  • MW: 325.40
  • Catalog: Angiotensin-converting Enzyme (ACE)
  • Density: 1.248 g/cm3
  • Boiling Point: 572.556ºC at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 300.071ºC

PTP1B-IN-3

PTP1B-IN-3 is a potent and selective PTP1B inhibitor with IC50s of 120 nM (PTP1B) and 120 nM (TCPTP), respectively.

  • CAS Number: 809272-64-8
  • MF: C12H7BrF2NO3P
  • MW: 362.06300
  • Catalog: Phosphatase
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

ZD-0892

ZD-0892 is a selective and potent inhibitor of a neutrophil elastase with Kis of 6.7 and 200 nM for human neutrophil elastase and porcine pancreatic elastase, respectively.

  • CAS Number: 171964-73-1
  • MF: C24H32F3N3O5
  • MW: 499.52
  • Catalog: Elastase
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

cm-10-18

CM-10-18 is a potent inhibitor of ER α-glucosidase. CM-10-18 demonstrated superior in vitro antiviral activity against representative viruses from four viral families causing hemorrhagic fever. CM-10-18 efficiently protected the lethality of dengue virus infection of mice.

  • CAS Number: 1159614-57-9
  • MF: C17H35NO5
  • MW: 333.46
  • Catalog: Glucokinase
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

HB007

HB007 is a small ubiquitin-related modifier 1 (SUMO1) degrader. HB007 induces ubiquitination and degradation of SUMO1, resulting in reduced tumor growth in vivo. HB007 can be used for the research of brain, breast, colon, and lung cancers[1][2].

  • CAS Number: 2387821-46-5
  • MF: C15H9ClN4Os
  • MW: 328.78
  • Catalog: E1/E2/E3 Enzyme
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Ecallantide

Ecallantide (DX-88) is a specific recombinant plasma kallikrein inhibitor. Ecallantide inhibits the production of bradykinin. Ecallantide can be used to prevent acute attacks of angioedema[1].

  • CAS Number: 460738-38-9
  • MF: C305H441N88O91S8
  • MW:
  • Catalog: Ser/Thr Protease
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

L-689502

L-689502 is a potent inhibitor of HIV-l protease with an IC50 of 1 nM.

  • CAS Number: 138483-63-3
  • MF: C39H51N3O7
  • MW: 673.83800
  • Catalog: HIV Protease
  • Density: 1.24g/cm3
  • Boiling Point: 886.9ºC at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 490.2ºC

Fitusiran

Fitusiran (ALN-AT3SC), an small interfering RNA, specifically targets antithrombin (AT) messenger RNA to lower production of AT in the liver. Fitusiran increases thrombin generation and has the potential for the research of the hemophilia[1].

  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

FAAH/cPLA2α-IN-1

FAAH/cPLA2α-IN-1 is a dual inhibitor of FAAH and cPLA2α with IC50s of 32 and 47 nM, respectively[1].

  • CAS Number: 1696401-38-3
  • MF: C19H26N4O5
  • MW: 390.43
  • Catalog: FAAH
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Anecortave Acetate

Anecortave acetate is a potent ocular angiostatic agent. Anecortave acetate inhibits neovascularization which is induced by many different angiogenic factors, and increases plasminogen activator inhibitor-1 (PAI-1) mRNA expression. Anecortave acetate can be used to research ocular neovascular diseases[1][2].

  • CAS Number: 7753-60-8
  • MF: C23H30O5
  • MW: 386.48100
  • Catalog: PAI-1
  • Density: 1.23g/cm3
  • Boiling Point: 551.8ºC at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 188.4ºC

PF-4191834

PF-4191834 (PF-04191834) is an orally active, noniron chelating, and non-redox inhibitor of the 5-Lipoxygenase (LOX) (IC50=229 nM), displays ~300-fold selectivity for 5-LOX over 12-LOX and 15-LOX, shows no activity toward the cyclooxygenase enzymes, and is effective in inflammation and pain[1].

  • CAS Number: 1029317-21-2
  • MF: C22H23N3O2S
  • MW: 393.50200
  • Catalog: 5-Lipoxygenase
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Ubenimex

Bestatin is a natural, broad-spectrum, and competitive aminopeptidase inhibitor.

  • CAS Number: 58970-76-6
  • MF: C16H24N2O4
  • MW: 308.373
  • Catalog: Aminopeptidase
  • Density: 1.2±0.1 g/cm3
  • Boiling Point: 604.7±55.0 °C at 760 mmHg
  • Melting Point: 245 °C (dec.)(lit.)
  • Flash Point: 319.5±31.5 °C

MMP3 inhibitor 1

MMP3 inhibitor 1 is a potent and highly selective MMP-3 inhibitor with an IC50 of 1 nM[1].

  • CAS Number: 312930-75-9
  • MF: C23H31N3O6S
  • MW: 477.57
  • Catalog: MMP
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Deoxyarbutin

Deoxyarbutin is a new effective lighten ingredient, can effectively inhibit tyrosinase activity and melanin synthesis to get significant and lasting lightening effect.

  • CAS Number: 53936-56-4
  • MF: C11H14O3
  • MW: 194.227
  • Catalog: Tyrosinase
  • Density: 1.2±0.1 g/cm3
  • Boiling Point: 349.8±32.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 165.3±25.1 °C

CCT129957

CCT129957 is an indole derivative and a potent phospholipase C-γ (PLC-γ) inhibitor with an IC50 of ~3 μM and a GC50 of 15 μM. CCT129957 inhibits Ca2+ release in squamous carcinoma cells at ~15 μM[1][2].

  • CAS Number: 883098-58-6
  • MF: C17H15N3O3
  • MW: 309.32
  • Catalog: Phospholipase
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Tarenflurbil

Tarenflurbil ((R)-Flurbiprofen) is the R-enantiomer of the racemate NSAID Flurbiprofen, Tarenflurbil ((R)-Flurbiprofen) inhibits the binding of [3H]9-cis-RA to RXRα LBD with IC50 of 75 μM.

  • CAS Number: 51543-40-9
  • MF: C15H13FO2
  • MW: 244.261
  • Catalog: RAR/RXR
  • Density: 1.2±0.1 g/cm3
  • Boiling Point: 376.2±30.0 °C at 760 mmHg
  • Melting Point: 110-113ºC
  • Flash Point: 181.3±24.6 °C

ML-​226

ML226 is a potent α/β hydrolase domain-containing protein 11 (ABHD11) inhibitor in vitro and in situ with IC50s of 15 and 0.68 nM, respectively[1].

  • CAS Number: 2055172-43-3
  • MF: C23H26N4O2
  • MW: 390.478
  • Catalog: MAGL
  • Density: 1.2±0.1 g/cm3
  • Boiling Point: 576.8±42.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 302.7±27.9 °C

Microcystin-LR

Microcystin-LR is a potent inhibitor of type 1 and type 2A protein phosphatases (PP1 and PP2A), with IC50s of 1.7 nM and 0.04 nM, respectively. Sequence: Ala-D-Ala-Leu-D-{Bas}-Arg-{Oaa}-D-{Ggu}.

  • CAS Number: 101043-37-2
  • MF: C49H74N10O12
  • MW: 995.172
  • Catalog: Peptides
  • Density: 1.3±0.1 g/cm3
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: 11 °C

NP-313

NP-313 is a potent antithrombotic agent that inhibits platelet aggregation and activation. NP-313 has dual inhibition of thromboxane A 2 synthesis and selective inhibition of SOCC-mediated Ca2+ inward flow[1].

  • CAS Number: 5397-78-4
  • MF: C12H8ClNO3
  • MW: 249.65000
  • Catalog: Thrombin
  • Density: 1.44g/cm3
  • Boiling Point: 427.7ºC at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 212.5ºC

Quinidine sulfate dihydrate

Quinidine sulfate dihydrate is a potent and selective inhibitor of cytochrome P450db and inhibits amphetamine metabolism in vivo[1]. Quinidine sulfate dihydrate enhances the cytotoxicity of vincristine (VCR) in tumor cells and especially in VCR-resistant sublines of P388 leukemia (P388/VCR) and human myelogenous leukemia[2].

  • CAS Number: 6591-63-5
  • MF: C20H24N2O2.1/2H2O4S.H2O
  • MW: 782.943
  • Catalog: Cytochrome P450
  • Density: N/A
  • Boiling Point: 992.5ºC at 760 mmHg
  • Melting Point: 212-214 °C (dec.)(lit.)
  • Flash Point: 554ºC