There are two classes of enzymes involved in histone methylation: methyltransferases and demethylases. While methyltransferases are responsible for establishing methylation patterns, demethylases are capable of removing methyl groups not only from histones but other proteins as well. Histone demethylases not only target methylated sites on histone tails but also interact with methylated sites on non-histone proteins, such as p53. Histone lysine demethylases (KDMs) are of interest as drug targets due to their regulatory roles in chromatin organization and their tight associations with diseases including cancer and mental disorders. JMJD1A (also named KDM3A) is a demethylasethat removes methyl from histone lysine H3K9. It plays important roles in various cellular processes, including spermatogenesis, energy metabolism, regulation of stem cell and gender display. Jumonji domain-containing 3 (Jmjd3) has been identified as a histone demethylase, which specifically catalyzes the removal of methylation from H3K27me3.


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15-PGDH 5 alpha Reductase 5-Lipoxygenase Acetyl-CoA Carboxylase Acyltransferase Adenosine Deaminase Adenosine Kinase Aldehyde Dehydrogenase (ALDH) Aldose Reductase Aminopeptidase Angiotensin-converting Enzyme (ACE) ATGL ATP Citrate Lyase Carbonic Anhydrase Carboxypeptidase Cathepsin CETP COMT Cytochrome P450 Dipeptidyl Peptidase Dopamine β-hydroxylase E1/E2/E3 Enzyme Elastase Enolase FAAH FABP Factor Xa Farnesyl Transferase Fatty Acid Synthase (FAS) FXR Glucokinase GSNOR Gutathione S-transferase HCV Protease Hexokinase HIF/HIF Prolyl-Hydroxylase HIV Integrase HIV Protease HMG-CoA Reductase (HMGCR) HSP Indoleamine 2,3-Dioxygenase (IDO) Isocitrate Dehydrogenase (IDH) Lactate Dehydrogenase LXR MAGL Mineralocorticoid Receptor Mitochondrial Metabolism MMP Nampt NEDD8-activating Enzyme Neprilysin PAI-1 PDHK PGC-1α Phosphatase Phosphodiesterase (PDE) Phospholipase Procollagen C Proteinase Proteasome Pyruvate Kinase RAR/RXR Renin ROR Ser/Thr Protease SGK Stearoyl-CoA Desaturase (SCD) Thrombin Tryptophan Hydroxylase Tyrosinase Xanthine Oxidase
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Lutidinic acid

2,4-PDCA (2,4 pyridine dicarboxylic acid) is a broad-spectrum inhibitor of 2OG oxygenase, including JmjC domain-containing family of histone demethylases (JHDMs). 2,4-PDCA is a target chemical in the field of bio-based plastics[1][2][3].

  • CAS Number: 499-80-9
  • MF: C7H5NO4
  • MW: 167.12
  • Catalog: Histone Demethylase
  • Density: 1.6±0.1 g/cm3
  • Boiling Point: 574.8±35.0 °C at 760 mmHg
  • Melting Point: 243-246 °C
  • Flash Point: 301.4±25.9 °C

CC-90011 besylate

CC-90011 is a potent and orally active LSD1 inhibitor.

  • CAS Number: 2097523-60-7
  • MF: C29H27F2N5O5S
  • MW: 595.62
  • Catalog: Histone Demethylase
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

GSK-J5

GSK-J5 is a potent inhibitor of Schistosome and worm. GSK-J5 increases schistosomula mortality and adult worm motility and mortality, as well as egg oviposition, in a dose- and time-dependent manner[1].

  • CAS Number: 1394854-51-3
  • MF: C24H27N5O2
  • MW: 417.50
  • Catalog: Histone Demethylase
  • Density: 1.2±0.1 g/cm3
  • Boiling Point: 581.2±50.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 305.3±30.1 °C

GSK-J2

GSK-J2 is an isomer of GSK-J1 that does not have any specific activity. GSK-J1 is a potent inhibitor of H3K27me3/me2-demethylases JMJD3/KDM6B and UTX/KDM6A.

  • CAS Number: 1394854-52-4
  • MF: C22H23N5O2
  • MW: 389.45
  • Catalog: Histone Demethylase
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

LSD1-IN-20

LSD1-IN-20 (compound 1) is a potent dual non-covalent LSD1/G9a inhibitor, with Ki values of 0.44 and 0.68 μM, respectively. LSD1-IN-20 shows antiproliferative activity in THP-1 leukemia cells and MDA-MB-231 breast cancer cells, with IC50 (72 h) of 0.51 and 1.60 μM, respectively[1].

  • CAS Number: 1239589-91-3
  • MF: C27H38N6O2
  • MW: 478.63
  • Catalog: Histone Demethylase
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

JHDM-IN-1

JHDM-IN-1 (Compound 1) is a Jumonji C domain-containing HDMs (JHDM) inhibitor with IC50s of 3.4, 4.3, 5.9, 10 and 43 μM against JMJD2C, JMJD2A, JMJD2E, PHF8 and JMJD3, respectively[1].

  • CAS Number: 1310809-17-6
  • MF: C27H29N3O6
  • MW: 491.54
  • Catalog: Histone Demethylase
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Namoline

Namoline, a γ-pyrone, is a selective and reversible Lysine-specific demethylase 1 (LSD1) inhibitor with an IC50 of 51 μM in a HRP-coupled enzymatic assay. Namoline impairs LSD1 demethylase activity and blocks cell proliferation. Namoline has the potential for androgen-dependent prostate cancer research[1].

  • CAS Number: 342795-11-3
  • MF: C10H3ClF3NO4
  • MW: 293.58
  • Catalog: Histone Demethylase
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

GSK2879552

GSK2879552 is an orally available, irreversible inhibitor of lysine specific demethylase 1 (LSD1), with potential antineoplastic activity.

  • CAS Number: 1401966-69-5
  • MF: C23H28N2O2
  • MW: 364.48100
  • Catalog: Histone Demethylase
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

KDM2/7-IN-1

KDM2/7-IN-1 (TC-E 5002) is a selective histone demethylase KDM2/7 subfamily inhibitor (IC50 values are 0.2, 1.2, 6.8, 55, 83, >100 and >120 μM for KDM7A, KDM7B, KDM2A, KDM5A, KDM4C, KDM6A and KDM4A respectively). KDM2/7-IN-1 inhibits growth of HeLa and KYSE-150 cancer cells in vitro[1].

  • CAS Number: 1453071-47-0
  • MF: C15H27NO4
  • MW: 285.37900
  • Catalog: Histone Demethylase
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: 87 °C
  • Flash Point: N/A

Daminozide

Daminozide(DMASA; DIMG; B 995), a plant growth regulator, selectively inhibits the KDM2/7 JmjC subfamily.IC50 Value: Target: KDM2/7 JmjCInhibition of shoot elongation in dwarf and tall peas by the 1,1-dimethylhydrazide of succinic acid (B-995) was correlated with the inhibition of the oxidation of tryptamine-2-C(14) to indoleacetaldehyde-2-C(14) in homogenates prepared from epicotyls of young plants treated with B-995. The growth-retarding action of B-995 is attributed to the formation of 1,1-dimethylhydrazine in vivo. This hydrazine strongly inhibited tryptamine oxidation by pea epicotyl homogenates[1]. Daminozide (N-(dimethylamino)succinamic acid, 160 Da), a plant growth regulator, selectively inhibits the KDM2/7 JmjC subfamily[2].in vitro: Essential oil content increased with increasing levels of gibberellins and decreased when gibberellin biosynthesis was blocked withdaminozide. With increasing levels of gibberellins, 1,8-cineole and camphor contents increased. Daminozide blocked the accumulation of alpha- and beta-thujone.[3].in vivo:

  • CAS Number: 1596-84-5
  • MF: C6H12N2O3
  • MW: 160.171
  • Catalog: Histone Demethylase
  • Density: 1.2±0.1 g/cm3
  • Boiling Point: 329.5±44.0 °C at 760 mmHg
  • Melting Point: 162-164 °C(lit.)
  • Flash Point: 153.1±28.4 °C

(±)-2-Hydroxyglutaric acid

α-Hydroxyglutaric acid (2-Hydroxyglutarate) disodium is an α-hydroxy acid form of glutaric acid. α-Hydroxyglutaric acid disodium is a competitive inhibitor of multiple α-ketoglutarate-dependent dioxygenases, including histone demethylases and the TET family of 5-methlycytosine (5mC) hydroxylases[1].

  • CAS Number: 40951-21-1
  • MF: C5H8O5
  • MW: 148.114
  • Catalog: Histone Demethylase
  • Density: 1.5±0.1 g/cm3
  • Boiling Point: 394.2±27.0 °C at 760 mmHg
  • Melting Point: >290ºC (dec.)
  • Flash Point: 206.4±20.2 °C

GSK-LSD1 2HCL

GSK-LSD1 is a LSD1 inhibitor. GSK-LSD1 reduces food intake and body weight, and improves insulin sensitivity and glycemic control in mouse models of obesity. GSK-LSD1 also ameliorates NAFLD. GSK-LSD1 inhibits SARS-CoV-2-triggered cytokine release in COVID-19 PBMCs. GSK-LSD1 also inhibits cancer growth and metastasis[1][2][3].

  • CAS Number: 1431368-48-7
  • MF: C14H20N2
  • MW: 216.322
  • Catalog: Histone Demethylase
  • Density: 1.1±0.1 g/cm3
  • Boiling Point: 347.4±42.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 206.0±19.7 °C

L-2-Hydroxyglutaric acid disodium

L-2-Hydroxyglutaric acid disodium is an epigenetic modifier and putative oncometabolite in renal cancer. L-2-Hydroxyglutaric acid disodium can inhibit histone demethylases and hence promote histone methylation[1]. L-2-Hydroxyglutaric acid inhibits mitochondrial creatine kinase (Mi-CK) activity with Km and Ki of 2.52 mM and 11.13 mM, respectively[2].

  • CAS Number: 63512-50-5
  • MF: C5H6Na2O5
  • MW: 192.078
  • Catalog: Histone Demethylase
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: >300ºC
  • Flash Point: N/A

Seclidemstat

Seclidemstat (SP-2577) is a potent LSD1 inhibitor, with a mean IC50 of 127 nM.

  • CAS Number: 1423715-37-0
  • MF: C20H23ClN4O4S
  • MW: 450.939
  • Catalog: Histone Demethylase
  • Density: 1.4±0.1 g/cm3
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

ML324

ML324 is a potent JMJD2 demethylase inhibitor with demonstrated antiviral activity.IC50 value: 920 nM(JMJD2E) [1]Target: JMJD2 demethylase inhibitorML324 is a probe molecule that displays submicromolar inhibitory activity toward JMJD2E (in vitro) and possesses excellent in vitro ADME properties. In contrast to previously reported inhibitors of the JMJD proteins, ML324 displays excellent cell permeability providing an opportunity for more extensive cell-based studies of JMJD2 enzymes to be undertaken. In addition, ML324 demonstrates potent anti-viral activity against both herpes simplex virus (HSV) and human cytomegalovirus (hCMV) infection via inhibition viral IE gene expression. ML324 suppresses the formation of HSV plaques, even at high MOI, and blocks HSV-1 reactivation in a mouse ganglia explant model of latently infected mice.

  • CAS Number: 1222800-79-4
  • MF: C21H23N3O2
  • MW: 349.426
  • Catalog: Histone Demethylase
  • Density: 1.2±0.1 g/cm3
  • Boiling Point: 590.1±50.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 310.7±30.1 °C

GSK-LSD1 Dihydrochloride

GSK-LSD1 Dihydrochloride is a potent, selective and irreversible lysine specific demethylase 1 (LSD1) inhibitor with an IC50 of 16 nM.

  • CAS Number: 2102933-95-7
  • MF: C14H22Cl2N2
  • MW: 289.24
  • Catalog: Histone Demethylase
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

α-Hydroxyglutaric acid-d4 disodium

α-Hydroxyglutaric acid-d4 (disodium) is the deuterium labeled α-Hydroxyglutaric acid disodium[1]. α-Hydroxyglutaric acid (2-Hydroxyglutarate) disodium is an α-hydroxy acid form of glutaric acid. α-Hydroxyglutaric acid disodium is a competitive inhibitor of multiple α-ketoglutarate-dependent dioxygenases, including histone demethylases and the TET family of 5-methlycytosine (5mC) hydroxylases[2].

  • CAS Number: 2483831-91-8
  • MF: C5H2D4Na2O5
  • MW: 196.10
  • Catalog: Histone Demethylase
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

QC6352

QC6352 is a potent KDM4C inhibitor with an IC50 of 35 nM.

  • CAS Number: 1851373-36-8
  • MF: C24H25N3O2
  • MW: 387.47
  • Catalog: Histone Demethylase
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Seclidemstat mesylate

Seclidemstat (SP-2577) mesylate is a potent noncompetitive and reversible KDM1A (LSD1) inhibitor (Ki=31 nM, IC50=13 nM). Seclidemstat mesylate promotes antitumor immunity in switch/sucrose nonfermentable (SWI/SNF) complex mutated ovarian cancer, as well as inhibit virus production, viral DNA replication, and late gene expression. Seclidemstat mesylate can be used for the research of Ewing Sarcoma[1][2].

  • CAS Number: 2044953-70-8
  • MF: C21H27ClN4O7S2
  • MW: 547.04
  • Catalog: Histone Demethylase
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

GSK690

GSK 690 is a reversible inhibitor of lysine specific demethylase 1 (LSD1), with a Kd value of 9 nM and a biochemical IC50 of 37 nM.

  • CAS Number: 2101305-84-2
  • MF: C24H23N3O
  • MW: 369.459
  • Catalog: Histone Demethylase
  • Density: 1.2±0.1 g/cm3
  • Boiling Point: 530.5±50.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 274.6±30.1 °C

Fenoldopam hydrochloride

Fenoldopam (SKF 82526) hydrochloride is a D1 receptor agonist and a novel lysine-specific demethylase 1 (LSD1) inhibitor (IC50=0.8974 μM). Fenoldopam hydrochloride shows anti-hypertensive effects, anti-cancer cell proliferation activity and can induce cells apoptosis[1][2][3].

  • CAS Number: 181217-39-0
  • MF: C16H17Cl2NO3
  • MW: 342.22
  • Catalog: Apoptosis
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

KDM5-C49

KDM5-C49 is a potent, selective KDM5 inhibitor with IC50 of 25, 30 and 59 nM for KDM5A, B and C, respectively; displays 25-100-fold selectivity over KDM6B, 2-30-fold selectivity over KDM4 family, but with less cell-permeablity.

  • CAS Number: 1596348-16-1
  • MF: C15H24N4O3
  • MW: 308.376
  • Catalog: Histone Demethylase
  • Density: 1.2±0.1 g/cm3
  • Boiling Point: 531.0±50.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 275.0±30.1 °C

KDM5B-IN-3

KDM5B-IN-3 (Compound 5) is a histone lysine specific demethylase 5B (KDM5B/JARID1B) inhibitor with an IC50 of 9.32 μM. KDM5B-IN-3 can be used for the research of gastric cancer[1].

  • CAS Number: 1385101-10-9
  • MF: C19H25ClN4O2
  • MW: 376.88
  • Catalog: Histone Demethylase
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

LSD1-IN-13

LSD1-IN-13 (compound 7e) is an orally active and potent LSD1 inhibitor, with an IC50 of 24.43 nM. LSD1-IN-13 can activate CD86 expression, with an EC50 of 470 nM. LSD1-IN-13 induces differentiation of AML (acute myeloid leukemia) cell lines[1].

  • CAS Number: 2170212-33-4
  • MF: C23H29N3O2S
  • MW: 411.56
  • Catalog: Histone Demethylase
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

JMJD6-IN-1

JMJD6-IN-1 (Compound 1-3) is a JMJD6 inhibitor, with an inhibition rate of 82% at 10 μM. JMJD6-IN-1 inhibits MCF-7 and HCC4006 cell proliferation with IC50s of 19.2 μM and 25.2 μM. JMJD6-IN-1 can be used for research of cancers[1].

  • CAS Number: 899548-78-8
  • MF: C16H11N3O2
  • MW: 277.28
  • Catalog: Histone Demethylase
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Bizine

Bizine, a Phenelzine analogue, is a potent and selective LSD1 inhibitor, with a b>Ki of 59 nM. Bizine can modulate bulk histone methylation in cancer cells. Bizine shows neuroprotective effects[1].

  • CAS Number: 1591932-50-1
  • MF: C18H25Cl2N3O
  • MW: 370.317
  • Catalog: Histone Demethylase
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

HDAC-IN-57

HDAC-IN-57 is an orally active inhibitor of histone deacetylases (HDAC), with IC50s of 2.07 nM, 4.71 nM, 2.4 nM and 107 nM for HDAC1, HDAC2, HDAC6, HDAC8, respectively. HDAC-IN-57 can inhibits LSD1, with IC50 of 1.34 μΜ. HDAC-IN-57 induces apoptosis, and has anti-tumor activity[1].

  • CAS Number: 2716217-79-5
  • MF: C21H19N3O4
  • MW: 377.39
  • Catalog: Apoptosis
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

CPI-455

CPI-455 is a specific KDM5 inhibitor.

  • CAS Number: 1628208-23-0
  • MF: C16H14N4O
  • MW: 278.309
  • Catalog: Histone Demethylase
  • Density: 1.3±0.1 g/cm3
  • Boiling Point: 488.7±55.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 249.3±31.5 °C

Vafidemstat

Vafidemstat is a dual lysine-specific histone demethylase (LSD1)/MAO-B inhibitor.

  • CAS Number: 1357362-02-7
  • MF: C19H20N4O2
  • MW: 336.39
  • Catalog: Histone Demethylase
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

GSK-J1

GSK-J1 is a potent inhibitor of H3K27me3/me2-demethylases JMJD3/KDM6B and UTX/KDM6A, with IC50 of 60 nM towards KDM6B. 

  • CAS Number: 1373422-53-7
  • MF: C22H23N5O2
  • MW: 389.450
  • Catalog: Histone Demethylase
  • Density: 1.3±0.1 g/cm3
  • Boiling Point: 608.9±55.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 322.0±31.5 °C