The TGF-β superfamily comprises TGF-βs, bone morphogenetic proteins (BMPs), activins and related proteins. These proteins were identified mainly through their roles in development; they regulate the establishment of the body plan and tissue differentiation through their effects on cell proliferation, differentiation and migration. There are eight vertebrate Smads: Smad1 to Smad8. Smad2 and Smad3 are activated through carboxy-terminal phosphorylation by the TGF-b and activin receptors TbRI and ActRIB, whereas Smad1, Smad5 and Smad8 are activated by ALK-1, ALK-2, BMP-RIA/ALK-3 and BMP-RIB/ALK-6 in response to BMP1–4 or other ligands.

TGF-β binds two receptor types, the TGF-β type I and type II receptors (TβRI and TβRII, respectively) to form the active signaling complex. The TβRII activates TβRI kinase activity by phosphorylating the TβRI, which then transmits the signal intracellularly by phosphorylating the Smad transcription factors. The Smads constitutively shuttle between the cytoplasm and nucleus, but signaling causes the Smads to accumulate predominantly in the nucleus where they bind DNA and other transcriptional machinery to regulate the expression of target genes. TGF-β also involves in the regulations of PI3K and MAPK signaling pathways.

Abnormalities of the TGF-beta receptors and SMADs have been detected in various tumors, including colorectal cancers and pancreatic cancers. In addition, TGF-β/BMP signaling is also involved in osteoblast differentiation, chondrocyte differentiation, skeletal development, cartilage formation, bone formation, bone homeostasis, and related human bone diseases caused by the disruption ofTGF-β/BMP signaling.

References:
[1] Derynck R, et al. Nature. 2003 Oct 9;425(6958):577-84.
[2] Clarke DC, et al. Trends Cell Biol. 2008 Sep;18(9):430-42.
[3] Wu M, et al. Bone Res. 2016 Apr 26;4:16009.


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Scaff10-8

Scaff10-8 is an inhibitor of AKAP-Lbc-RhoA interaction that acts by promoting the translocation of aquaporin-2 to the plasma membrane of renal collecting duct principal cells.

  • CAS Number: 777857-56-4
  • MF: C22H18O6
  • MW: 378.37
  • Catalog: ROCK
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Psychosine-d5

Psychosine-d5 is deuterium labeled Psychosine. Psychosine, a substrate of the galactocerebrosidase (GALC) enzyme, is a potential biomarker for Krabbe disease. Psychosine is a highly cytotoxic lipid, capable of inducing cell death in a wide variety of cell.

  • CAS Number: 2260670-12-8
  • MF: C24H42D5NO7
  • MW: 466.66
  • Catalog: PKC
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

1,2-Didecanoylglycerol

1,2-Didecanoylglycerol, a synthetic diacylglycerol, is metabolized by platelets to 1,2-didecanoylphosphatidic acid (PA10) and activates protein kinase C (PKC)[1][2].

  • CAS Number: 17863-69-3
  • MF: C23H44O5
  • MW: 400.59
  • Catalog: PKC
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

H-PHE-LYS-LYS-SER-PHE-LYS-LEU-NH2

FKKSFKL-NH2 is a protein kinase C-selective peptide. FKKSFKL-NH2 can be used for the research of various biochemical[1].

  • CAS Number: 137168-33-3
  • MF: C45H73N11O8
  • MW: 896.130
  • Catalog: PKC
  • Density: 1.2±0.1 g/cm3
  • Boiling Point: 1275.7±65.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 725.3±34.3 °C

1-Stearoyl-2-arachidonoyl-sn-glycerol

1-Stearoyl-2-arachidonoyl-sn-glycerol is a diacylglycerol (DAG) containing polyunsaturated fatty acids. 1-Stearoyl-2-arachidonoyl-sn-glycerol can activate PKC. 1-Stearoyl-2-arachidonoyl-sn-glycerol also can augment nonselective cation channel (NSCC) activity[1][2].

  • CAS Number: 65914-84-3
  • MF: C41H72O5
  • MW: 645.007
  • Catalog: PKC
  • Density: 0.9±0.1 g/cm3
  • Boiling Point: 689.2±55.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 190.3±25.0 °C

PF-03622905

PF-03622905 is a potent and ATP-competitive PKC inhibitor with IC50s of 5.6 nM, 14.5 nM, 13 nM, 37.7 nM, and 74.1 nM for PKCα, PKCβI, PKCβII, PKCγ, and PKCθ, respectively. PF-03622905 shows high specificity for PKC over other protein kinases[1].

  • CAS Number: 1072100-15-2
  • MF: C24H35N7O3
  • MW: 469.58
  • Catalog: PKC
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

1-Oleoyl-2-acetyl-rac-glycerol

1-Oleoyl-2-acetyl-sn glycerol is a synthetic, cell permeable diacylglycerol analog. 1-Oleoyl-2-acetyl-sn glycerol activates calcium-dependent protein kinase C (PKC) and induces the superoxide-production.

  • CAS Number: 86390-77-4
  • MF: C23H42O5
  • MW: 398.577
  • Catalog: PKC
  • Density: 1.0±0.1 g/cm3
  • Boiling Point: 490.0±15.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 152.3±13.9 °C

UCN-02

UCN-02 (7-epi-Hydroxystaurosporine) is a selective protein kinase C (PKC) inhibitor produced by Streptomyces strain N-12, with IC50s of 62 nM and 250 nM for PKC and protein kinase A (PKA), respectively. UCN-02 (7-epi-Hydroxystaurosporine) displays cytotoxic effect on the growth of HeLa S3 cells[1][2].

  • CAS Number: 121569-61-7
  • MF: C28H26N4O4
  • MW: 482.537
  • Catalog: PKC
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

BDP5290

BDP5290 is a potent inhibitor of both ROCK and MRCK with IC50s of 5 nM, 50 nM, 10 nM and 100 nM for ROCK1, ROCK2, MRCKα and MRCKβ, respectively.

  • CAS Number: 1817698-21-7
  • MF: C17H18ClN7O
  • MW: 371.82
  • Catalog: ROCK
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

PKC-IN-4

PKC-IN-4 (compound 7l) is a potent and orally active aPKC inhibitor with an IC50 of 0.52 µM. PKC-IN-4 inhibits TNF-α induced NF-κB activity in vitro. PKC-IN-4 blocks VEGF- and TNFα-induced permeability across the retinal vasculature[1].

  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Calphostin C

Calphostin C is a potent and specific inhibitor of protein kinase C. Calphostin C is an antitumor antibiotic. Calphostin C has 1000 times more inhibitory to protein kinase C with an IC50 of 0.05 μM than other protein kinases. Calphostin C induces apoptosis in some tumor cell lines. Calphostin C has potent cytotoxic activity and antitumor activity[1].

  • CAS Number: 121263-19-2
  • MF: C44H38O14
  • MW: 790.764
  • Catalog: Apoptosis
  • Density: 1.5±0.1 g/cm3
  • Boiling Point: 1039.8±65.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 317.0±27.8 °C

PKC β pseudosubstrate

PKC β pseudosubstrate is a selective cell-permeable inhibitor of PKC[1].

  • CAS Number: 172308-76-8
  • MF: C177H294N62O38S3
  • MW: 3994.82
  • Catalog: PKC
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

EMT inhibitor-1

EMT inhibitor-1 is an inhibitor of of Hippo, TGF-β, and Wnt signaling pathways with antitumor activities.

  • CAS Number: 1638526-21-2
  • MF: C12H12Cl2N2O2S
  • MW: 319.21
  • Catalog: Hippo (MST)
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

HA-100 hydrochloride

HA-100 dihydrochloride is a potent protein kinase inhibitor, with IC50s of 4 μM, 8 μM, 12 μM and 240 μM for cGMP-dependent protein kinase (PKG), cAMP-dependent protein kinase (PKA), protein kinase C (PKC) and MLC-kinase, respectively. HA-100 dihydrochloride also used as a ROCK inhibitor[1][2].

  • CAS Number: 210297-47-5
  • MF: C13H17Cl2N3O2S
  • MW: 350.26
  • Catalog: ROCK
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

ɛPKC(85–92),Myristoylated

?PKC(85–92),Myristoylated is a cell permeable myristic acid conjugated PKC? peptide activator that enhances NO release in cultured human umbilical vein endothelial cells (HUVECs)[1].

  • CAS Number: 1072301-79-1
  • MF: C53H80N10O15
  • MW: 1097.26
  • Catalog: PKC
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

ζ-Stat

ζ-Stat (NSC37044) is a specific and atypical PKC-ζ inhibitor, with an IC50 of 5 μM. ζ-Stat can reduce melanoma cell lines proliferation and induce apoptosis, and has antitumor activity in vitro[1][2].

  • CAS Number: 3316-02-7
  • MF: C10H8O10S3
  • MW: 384.36000
  • Catalog: Apoptosis
  • Density: 2.019g/cm3
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

SMAD3

SIS3 free base is a potent and selective inhibitor of TGF- beta1-induced Smad3 phosphorylation with an IC50 of 3 μM. SIS3 free base increases luciferase activity of p3TP-lux by abrogating the overexpression of constitutively active form of ALK-5[1].

  • CAS Number: 1009104-85-1
  • MF: C28H27N3O3
  • MW: 453.532
  • Catalog: ALK
  • Density: 1.2±0.1 g/cm3
  • Boiling Point: 721.8±60.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 390.3±32.9 °C

LY364947

LY-364947 is a potent ATP-competitive inhibitor of TGFβR-I with IC50 of 59 nM, and exhibits 7-fold selectivity over TGFβR-II.

  • CAS Number: 396129-53-6
  • MF: C17H12N4
  • MW: 272.304
  • Catalog: TGF-β Receptor
  • Density: 1.3±0.1 g/cm3
  • Boiling Point: 490.8±45.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 268.7±15.1 °C

Fasudil

Fasudil is a potent inhibitor of ROCK1, PKA, PKC, and MLCK with Ki of 0.33 μM, 1.0 μM, 9.3 μM and 55 μM, respectively.

  • CAS Number: 103745-39-7
  • MF: C14H17N3O2S
  • MW: 291.37
  • Catalog: Autophagy
  • Density: 1.3±0.1 g/cm3
  • Boiling Point: 506.2±60.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 259.9±32.9 °C

3-Methyl-2-butenoic acid [8-hydroxymethyl-1,5-dimethyl-5-(4-methyl-3-pentenyl)-12-oxabicyclo[9.1.0]dodeca-3,7-dien-2-yl] ester

Vibsanin A, a protein kinase C (PKC) activator, exhibits anti-proliferative activity against human cancer cell lines. Vibsanin A is also a HSP90 inhibitor[1].

  • CAS Number: 72506-14-0
  • MF: C25H38O4
  • MW: 402.57
  • Catalog: HSP
  • Density: 1.1±0.1 g/cm3
  • Boiling Point: 501.0±50.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 158.5±23.6 °C

EW-7197 Hydrochloride

Vactosertib Hydrochloride (EW-7197 Hydrochloride) is a small-molecule ATP-competitive inhibitor of TGFβRI (ALK5) with an IC50 of 12.9 nM.

  • CAS Number: 1352610-25-3
  • MF: C22H19ClFN7
  • MW: 435.88
  • Catalog: TGF-β Receptor
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

CCG-232964

CCG-232964 is an orally active inhibitor of Rho/MRTF/SRF. CCG-232964 inhibits LPA-induced CTGF gene expression[1].

  • CAS Number: 2349373-70-0
  • MF: C15H15ClN2O3S
  • MW: 338.81
  • Catalog: ROCK
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Belumosudil mesylate

Belumosudil mesylate (KD025 mesylate) is a selective inhibitor of ROCK2 with IC50s of 105 nM and 24 µM for ROCK2 and ROCK1, respectively. Anti-fibrotic properties[1].

  • CAS Number: 2109704-99-4
  • MF: C27H28N6O5S
  • MW: 548.61
  • Catalog: ROCK
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

SB505124

SB-505124 is a selective inhibitor of TGFβR with IC50 of 129 nM and 47 nM for ALK4, ALK5, respectively, and it does not inhibits ALK1, 2, 3, or 6 but ALK7.

  • CAS Number: 694433-59-5
  • MF: C20H21N3O2
  • MW: 335.400
  • Catalog: TGF-β Receptor
  • Density: 1.2±0.1 g/cm3
  • Boiling Point: 509.7±50.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 180.5±20.4 °C

Kartogenin

Kartogenin is an inducer of differentiation of human mesenchymal stem cells into chondrocytes.

  • CAS Number: 4727-31-5
  • MF: C20H15NO3
  • MW: 317.338
  • Catalog: TGF-beta/Smad
  • Density: 1.3±0.1 g/cm3
  • Boiling Point: 464.4±38.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 234.6±26.8 °C

K 02288

K02288 is a potent inhibitor of ALK, and inhibits ALK1/2/3/6 with IC50s of 1.8/1.1/34.4/6.3 nM; K02288 is less potent against ALK4/5, with IC50s of 302 nM and 321 nM.

  • CAS Number: 1431985-92-0
  • MF: C20H20N2O4
  • MW: 352.384
  • Catalog: ALK
  • Density: 1.2±0.1 g/cm3
  • Boiling Point: 522.2±50.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 269.6±30.1 °C

1-amino-8-naphthol-2,4-disulfonic acid monosodium salt

CaMKP inhibitor sodium is a selective inhibitor of ROCK1 with an IC50 value of 14 nM.

  • CAS Number: 52789-62-5
  • MF: C10H8NNaO7S2
  • MW: 341.29
  • Catalog: ROCK
  • Density: 1.477 g/mL at 25 °C(lit.)
  • Boiling Point: 270-272 °C(lit.)
  • Melting Point: 12-16 °C(lit.)
  • Flash Point: >230 °F

SB525334

SB 525334 is a potent and selective transforming growth factor β1 receptor (ALK5) inhibitor with an IC50 of 14.3 nM.

  • CAS Number: 356559-20-1
  • MF: C21H21N5
  • MW: 343.425
  • Catalog: TGF-β Receptor
  • Density: 1.2±0.1 g/cm3
  • Boiling Point: 540.5±45.0 °C at 760 mmHg
  • Melting Point: 159 °C
  • Flash Point: 238.6±21.7 °C

Elezanumab

Elezanumab (ABT-555; AE12-1Y-QL) is a human monoclonal antibody that selectively targets repulsive guidance molecule A (RGMa). Elezanumab potently inhibited RGMa mediated BMP signalling via the SMAD1/5/8 pathway, with an IC50 around 97 pM. Elezanumab promotes neuroregeneration and neuroprotection in neuronal injury and demyelination models binds N-terminal RGMa, blocks BMP signaling and lacks RGMc cross-reactivity. elezanumab has neuroregenerative and neuroprotective activities without impact on iron metabolism[1][2].

  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

ROCK-IN-8

ROCK-IN-8 (Example 4) is a ROCK inhibitor, with an IC50 value less than 100 nM. ROCK-IN-8 has anti-inflammatory activity. ROCK-IN-8 can be used for research of respiratory and gastro-intestinal diseases[1].

  • CAS Number: 1621103-79-4
  • MF: C30H25FN4O4S
  • MW: 556.61
  • Catalog: ROCK
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A