The PI3K/Akt/mTOR signaling pathways is crucial to many aspects of cell growth and survival, in physiological as well as in pathological conditions. PI3Ks constitute a lipid kinase family. Class I PI3Ks are heterodimers composed of a catalytic (CAT) subunit (i.e., p110) and an adaptor/regulatory subunit (i.e., p85), and can be further divided into two subclasses: subclass IA (PI3Kα, β, and δ), which is activated by receptors with protein tyrosine kinase activity, and subclass IB (PI3Kγ), which is activated by receptors coupled with G proteins. Akt kinases belong to the AGC kinase family, related to AMP/GMP kinases and protein kinase C. mTOR is a key protein evolutionarily conserved from yeast to man and is essential for life. The mTORC1 complex is made up of mTOR, Raptor, mLST8, and PRAS40, and the mTORC2 complex is composed of mTOR, Rictor, Sin1, and mLST8.

Upon ligand binding, phosphorylated tyrosine residing in activated RTKs will bind to p85, then release the catalytic subunit p110. Activated p110 phosphorylated the PIP2 into the second messenger PIP3, and this reaction can be reversed by the PI3K antagonist PTEN. PIP3 will recruit the downstream Akt to inner membranes and phosphorylates Akt on its serine/threonine kinase sites (Thr308 and Ser473). Activated Akt is involved in the downstream mTORC1 mediated response to biogenesis of protein and ribosome.

Many genes belonging to the PI3K/Akt pathway have been implicated in the pathophysiology of solid tumors and sensitivity/resistance to chemotherapy. More and more studies are now focusing on the translational relevance of targeting these pathways in cancer therapy.

References:
[1] Porta C, et al. Front Oncol. 2014 Apr 14;4:64.
[2] Follo MY, et al. Adv Biol Regul. 2015 Jan;57:10-6.
[3] Li X, et al. Oncotarget. 2016 May 31;7(22):33440-50.


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2B-(SP) (TFA)

2B-(SP) is a eIF2B-based substrate for glycogen synthase kinase-3 (GSK-3). 2B-(SP) is readily phosphorylated by both the α and β isoforms of GSK-3[1].

  • CAS Number: 186901-17-7
  • MF: C71H123N26O29P
  • MW: 1835.87000
  • Catalog: GSK-3
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

PI3Kα-IN-9

PI3Kα-IN-9 (compound 27) is a selective, long-acting and oral active PI3Kα inhibitor with IC50 values of 4.4, 128, 146 and 153 nM for PI3Kα, PI3Kγ, PI3Kδ and PI3Kβ, respectively. PI3Kα-IN-9 has antiproliferative activity and induces apoptosis. PI3Kα-IN-9 can be used for cancer research[1].

  • CAS Number: 2715287-67-3
  • MF: C18H21N7O3
  • MW: 383.40
  • Catalog: Apoptosis
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

ATM Inhibitor-5

ATM Inhibitor-5 [formula (1)] is a potent inhibitor of serine/threonine protein kinase ATM (extracted from patent WO2022058351A1)[1].

  • CAS Number: 2495096-26-7
  • MF: C23H21FN6O3
  • MW: 448.45
  • Catalog: ATM/ATR
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Manzamine A hydrochloride

Manzamine A hydrochloride, an orally active beta-carboline alkaloid, inhibits specifically GSK-3β and CDK-5 with IC50s of 10.2 and 1.5μM, respectively. Manzamine A hydrochloride targets vacuolar ATPases and inhibits autophagy in pancreatic cancer cells. Manzamine A hydrochloride has antimalarial and anticancer activities. Manzamine A hydrochloride also shows potent activity against HSV-1[1][2][3][4].

  • CAS Number: 104264-80-4
  • MF: C36H45ClN4O
  • MW: 585.22
  • Catalog: HIV
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Cimigenol-3-O-alpha-L-arabinoside

Cimiracemoside C is an active component of Cimicifuga racemosa, activates AMPK, has the potential activity against diabetes[1].

  • CAS Number: 256925-92-5
  • MF: C35H56O9
  • MW: 620.814
  • Catalog: AMPK
  • Density: 1.3±0.1 g/cm3
  • Boiling Point: 740.3±60.0 °C at 760 mmHg
  • Melting Point: 258-260℃
  • Flash Point: 401.5±32.9 °C

GDC-0068

Ipatasertib (GDC-0068) is a highly selective and ATP-competitive pan-Akt inhibitor with IC50s of 5, 18 and 8 nM for Akt1, Akt2 and Akt3, respectively.

  • CAS Number: 1001264-89-6
  • MF: C24H32ClN5O2
  • MW: 457.996
  • Catalog: Akt
  • Density: 1.3±0.1 g/cm3
  • Boiling Point: 669.4±55.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 358.7±31.5 °C

YM201636

YM-201636 is a potent and selective PIKfyve inhibitor with an IC50 of 33 nM. YM-201636 also inhibits p110α with IC50 of 3.3 μM.

  • CAS Number: 371942-69-7
  • MF: C25H21N7O3
  • MW: 467.479
  • Catalog: Autophagy
  • Density: 1.4±0.1 g/cm3
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

SKLB-197

SKLB-197 showed an IC50 value of 0.013 μM against ATR but very weak or no activity against other 402 protein kinases. It displayed potent antitumor activity against ATM-deficent tumors both in vitro and in vivo.

  • CAS Number: 2713577-16-1
  • MF: C25H24N6O
  • MW: 424.50
  • Catalog: ATM/ATR
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

OTSSP167

OTSSP167 is a highly potent MELK inhibitor with IC50 value of 0.41 nM.

  • CAS Number: 1431697-89-0
  • MF: C25H28Cl2N4O2
  • MW: 487.421
  • Catalog: MELK
  • Density: 1.3±0.1 g/cm3
  • Boiling Point: 619.0±55.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 328.2±31.5 °C

Zandelisib

Zandelisib is a phosphatidylinositol 3-kinase (PI3K) inhibitor extracted from patent WO2019183226 A1, Compound Example 1. Zandelisib selectively inhibits p110δ with an IC50 of 3.5 nM. Zandelisib functions as an antineoplastic[1][2].

  • CAS Number: 1401436-95-0
  • MF: C31H38F2N8O
  • MW: 576.68
  • Catalog: PI3K
  • Density: N/A
  • Boiling Point: 729.2±70.0 °C(Predicted)
  • Melting Point: N/A
  • Flash Point: N/A

TAS-117

TAS-117 is a highly selective, non-ATP competitive pan-Akt inhibitor with IC50 of 4.8/1.6/44 nM for Akt1/Akt2/Akt3 respectively; shows minimal inhibitory activities against others kinases including PI3K, PDK1, and mTOR; inhibits Akt kinase activity but not p-Akt, blocks basal phosphorylation of Akt and downstream p-FKHR/FKHRL1; induces significant cytotoxicity in MM cells associated with inhibition of IL6 secretion; triggers apoptosis and autophagy, induces ER stress response, inhibits human MM cell growth in murine xenograft models. Solid Tumors Phase 1 Clinical

  • CAS Number: 1402602-94-1
  • MF: C26H24N4O2
  • MW: 424.494
  • Catalog: Apoptosis
  • Density: 1.4±0.1 g/cm3
  • Boiling Point: 655.8±65.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 350.4±34.3 °C

ARN25068

ARN25068 is a sub-micromolar inhibitor of the three protein kinases, GSK-3β, FYN and DYRK1A to tackle tau hyperphosphorylation[1].

  • CAS Number: 2649882-80-2
  • MF: C19H18N6S
  • MW: 362.45
  • Catalog: GSK-3
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

KP-23172

KP-23172 is a PI3-K/Akt pathway inhibitor extracted from patent US7196083[1].

  • CAS Number: 331253-86-2
  • MF: C10H4N6O
  • MW: 224.17800
  • Catalog: Akt
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

IM-12

IM-12 is an inhibitor of GSK-3β, with an IC50 of 53 nM, and also enhances Wnt signalling.

  • CAS Number: 1129669-05-1
  • MF: C22H20FN3O2
  • MW: 377.411
  • Catalog: GSK-3
  • Density: 1.3±0.1 g/cm3
  • Boiling Point: 600.2±55.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 316.8±31.5 °C

PI3K-IN-46

PI3K-IN-46 (Intermediate 4) is an intermediate in the synthesis of PI3K inhibitor (2-imino-azolinone-vinyl fused-benzene derivative) that can be used for the research of autoimmune disorders, cardiovascular diseases, and neurodegenerative diseases[1].

  • CAS Number: 304645-61-2
  • MF: C13H9N3OS
  • MW: 255.30
  • Catalog: PI3K
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

TCS 2002

GSK-3β inhibitor 9 (Compound 9b) is a highly selective, orally bioavailable and potent GSK-3β inhibitor with the IC50 of 35 nM. GSK-3β inhibitor 9 shows good pharmacokinetic profiles including favorable BBB penetration. GSK-3β inhibitor 9 can be used for the research of Alzheimer’s disease[1].

  • CAS Number: 1005201-24-0
  • MF: C18H14N2O3S
  • MW: 338.38000
  • Catalog: GSK-3
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

GSK621

GSK621 is a specific AMPK activator, with IC50 values of 13-30 μM for AML cells. GSK621 induces autophagy and apoptosis. GSK621 induces eiF2α phosphorylation-a hallmark of UPR activation[1].

  • CAS Number: 1346607-05-3
  • MF: C26H20ClN3O5
  • MW: 489.907
  • Catalog: Apoptosis
  • Density: 1.4±0.1 g/cm3
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

GSK3 Inhibitor XIII

GSK-3 Inhibitor XIII is a potent and ATP-competitive GSK-3 inhibitor with a Ki of 24 nM[1][1][2].

  • CAS Number: 404828-08-6
  • MF: C18H15N5
  • MW: 301.34500
  • Catalog: GSK-3
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

BI-1622

BI-1622 is an orally active, potent and highly selective HER2 (ERBB2) inhibitor, with an IC50 of 7 nM. BI-1622 shows greater than 25-fold selectivity over EGFR. BI-1622 shows high antitumor efficacy in vivo in xenograft mouse tumor models with engineered H2170 and PC9 cells and had a favorable drug metabolism and pharmacokinetics profile[1].

  • CAS Number: 2681392-19-6
  • MF: C26H24N10O2
  • MW: 508.53
  • Catalog: CDK
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

VP3.15 dihydrobromide

VP3.15 dihydrobromide is a potent, orally bioavailable and CNS-penetrant dual phosphodiesterase (PDE)7- glycogen synthase kinase (GSK)3 inhibitor, with IC50s of 1.59 μM and 0.88 μM for PDE7 and GSK-3, respectively. VP3.15 dihydrobromide has neuroprotective and neuroreparative activities, thus as potential combined anti-inflammatory and pro-remyelinating therapies for multiple sclerosis (MS)[1].

  • CAS Number: 1281681-33-1
  • MF: C20H24Br2N4OS
  • MW: 528.30
  • Catalog: Phosphodiesterase (PDE)
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

3-Methyladenine-d3

3-Methyladenine-d3 is the deuterium labeled 3-Methyladenine[1]. 3-Methyladenine (3-MA) is a PI3K inhibitor. 3-Methyladenine is a widely used inhibitor of autophagy via its inhibitory effect on class III PI3K[2].

  • CAS Number: 110953-39-4
  • MF: C6H4D3N5
  • MW: 152.17200
  • Catalog: Autophagy
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

FD2056

FD2056 is a potent and orally active PI3K inhibitor. FD2056 inhibits PI3Kα/PI3Kβ/PI3Kγ/PI3Kδ with IC50s of 0.30, 0.80, 1.10, 0.42 nM. FD2056 also inhibits CDK2-CyclinA2 and CDK4-CyclinD3 with IC50 of 115.95 and 2782.15 nM. FD2056 inhibits breast cancer cell proliferation with IC50s of 1.06, 0.04, 1.40 μM for MDA-MB-231, MDA-MB-468, MCF-7 cells. FD2056 also induces cancer apoptosis and inhibits tumor growth[1].

  • CAS Number: 2685870-87-3
  • MF: C23H17ClN6O2S
  • MW: 476.94
  • Catalog: PI3K
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

CCT 365623 hydrochloride

CCT 365623 (CCT365623) hydrochloride is a potent, orally active small molecule inhibitor of lysyl oxidase (LOX) with IC50 of 0.89 uM; inhibits LOX actvity in living cell system (MDCK cysts) at 5 uM, reduces EGFR retention at the cell surface, suppresses EGFR and AKT phosphorylation driven by EGF, also activates SMAD2 and downregulates MATN2; delays the growth of primary and metastatic tumour cells in vivo.

  • CAS Number: 2126136-98-7
  • MF: C18H18ClNO4S3
  • MW: 443.975
  • Catalog: EGFR
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Chrysomycin A

Chrysomycin A (Chr-A), an antibiotic, can be obtained from Streptomyces. Chrysomycin A exhibits antitumor and anti-tuberculous and MRSA activities. As for glioblastoma, Chrysomycin A inhibits the proliferation, migration, and invasion of cancer cells through the Akt/GSK-3β/β-catenin signaling pathway[1].

  • CAS Number: 82196-88-1
  • MF: C28H28O9
  • MW: 508.517
  • Catalog: Bacterial
  • Density: 1.4±0.1 g/cm3
  • Boiling Point: 799.7±60.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 269.6±26.4 °C

Gomisin J

Gomisin J is a small molecular weight lignan found in Schisandra chinensis and has been demonstrated to have vasodilatory activity[1]. Gomisin J suppresses lipid accumulation by regulating the expression of lipogenic and lipolytic enzymes and inflammatory molecules through activation of AMPK, LKB1 and Ca2+/calmodulin-dependent protein kinase II and inhibition of fetuin-A in HepG2 cells. gomisin J has potential benefits in treating nonalcoholic fatty liver disease[2].

  • CAS Number: 66280-25-9
  • MF: C22H28O6
  • MW: 388.454
  • Catalog: AMPK
  • Density: 1.161
  • Boiling Point: 587.5±50.0 °C at 760 mmHg
  • Melting Point: 148-149 ºC
  • Flash Point: 309.1±30.1 °C

PS48

PS48 is an activator of PDK1 with an AC50 of 8 μM.

  • CAS Number: 1180676-32-7
  • MF: C17H15ClO2
  • MW: 286.753
  • Catalog: PDK-1
  • Density: 1.2±0.1 g/cm3
  • Boiling Point: 444.1±24.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 222.4±22.9 °C

MEK/PI3K-IN-2

MEK/PI3K-IN-2 (compound 6s) is a potent MEK/PI3K inhibitor, with IC50 values of 352 nM (MEK1), 107 nM (PI3Kα), and 137 nM (PI3Kδ), respectively. MEK/PI3K-IN-2 suppresses pAKT and pERK1/2 levels. MEK/PI3K-IN-2 shows anti-proliferative activity against tumor cell lines[1].

  • CAS Number: 2281803-33-4
  • MF: C38H41F5IN9O7
  • MW: 957.68
  • Catalog: PERK
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Pomiferin

Pomiferin, a flavonoid from the fruits of Maclura pomifera, acts as an potential inhibitor of HDAC, with an IC50 of 1.05 μM, and also potently inhibits mTOR (IC50, 6.2 µM).

  • CAS Number: 572-03-2
  • MF: C25H24O6
  • MW: 420.45400
  • Catalog: HDAC
  • Density: 1.314g/cm3
  • Boiling Point: 673.5ºC at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 233.3ºC

AZ2

AZ2 is a highly selective PI3Kγ inhibitor (The pIC50 value for PI3Kγ is 9.3). AZ2 can be used for the research of inflammatory and immune diseases[1].

  • CAS Number: 2231760-33-9
  • MF: C20H23N3O2S
  • MW: 369.48
  • Catalog: PI3K
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

MCX 28

MCX 28, a triple PI3K/mTOR/PIM inhibitor, displays low nanomolar activity.

  • CAS Number: 1414453-58-9
  • MF: C25H19N5O4S3
  • MW: 549.64
  • Catalog: mTOR
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A