Metabolic pathways are enzyme-mediated biochemical reactions that lead to biosynthesis (anabolism) or breakdown (catabolism) of natural product small molecules within a cell or tissue. In each pathway, enzymes catalyze the conversion of substrates into structurally similar products. Metabolic processes typically transform small molecules, but also include macromolecular processes such as DNA repair and replication, and protein synthesis and degradation. Metabolism maintains the living state of the cells and the organism.

Proteases are used throughout an organism for various metabolic processes. Proteases control a great variety of physiological processes that are critical for life, including the immune response, cell cycle, cell death, wound healing, food digestion, and protein and organelle recycling. On the basis of the type of the key amino acid in the active site of the protease and the mechanism of peptide bond cleavage, proteases can be classified into six groups: cysteine, serine, threonine, glutamic acid, aspartate proteases, as well as matrix metalloproteases. Proteases can not only activate proteins such as cytokines, or inactivate them such as numerous repair proteins during apoptosis, but also expose cryptic sites, such as occurs with β-secretase during amyloid precursor protein processing, shed various transmembrane proteins such as occurs with metalloproteases and cysteine proteases, or convert receptor agonists into antagonists and vice versa such as chemokine conversions carried out by metalloproteases, dipeptidyl peptidase IV and some cathepsins. In addition to the catalytic domains, a great number of proteases contain numerous additional domains or modules that substantially increase the complexity of their functions.

Imbalances in metabolic activities have been found to be critical in a number of pathologies, such as cardiovascular diseases, inflammation, cancer, and neurodegenerative diseases.

References:
[1] Turk B, et al. EMBO J. 2012 Apr 4;31(7):1630-43.
[2] Eatemadi A, et al. Biomed Pharmacother. 2017 Feb;86:221-231.


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15-PGDH 5 alpha Reductase 5-Lipoxygenase Acetyl-CoA Carboxylase Acyltransferase Adenosine Deaminase Adenosine Kinase Aldehyde Dehydrogenase (ALDH) Aldose Reductase Aminopeptidase Angiotensin-converting Enzyme (ACE) ATGL ATP Citrate Lyase Carbonic Anhydrase Carboxypeptidase Cathepsin CETP COMT Cytochrome P450 Dipeptidyl Peptidase Dopamine β-hydroxylase E1/E2/E3 Enzyme Elastase Enolase FAAH FABP Factor Xa Farnesyl Transferase Fatty Acid Synthase (FAS) FXR Glucokinase GSNOR Gutathione S-transferase HCV Protease Hexokinase HIF/HIF Prolyl-Hydroxylase HIV Integrase HIV Protease HMG-CoA Reductase (HMGCR) HSP Indoleamine 2,3-Dioxygenase (IDO) Isocitrate Dehydrogenase (IDH) Lactate Dehydrogenase LXR MAGL Mineralocorticoid Receptor Mitochondrial Metabolism MMP Nampt NEDD8-activating Enzyme Neprilysin PAI-1 PDHK PGC-1α Phosphatase Phosphodiesterase (PDE) Phospholipase Procollagen C Proteinase Proteasome Pyruvate Kinase RAR/RXR Renin ROR Ser/Thr Protease SGK Stearoyl-CoA Desaturase (SCD) Thrombin Tryptophan Hydroxylase Tyrosinase Xanthine Oxidase
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20(R)-Ginsenoside Rh2

20(R)-Ginsenoside Rh2, a matrix metalloproteinase (MMP) inhibitor, acts as a cell antiproliferator. It has anticancer effects via blocking cell proliferation and causing G1 phase arrest. 20(R)-Ginsenoside Rh2 induces apoptosis, and has anti-inflammatory and antioxidative activity[1][2][3].

  • CAS Number: 112246-15-8
  • MF: C36H62O8
  • MW: 622.873
  • Catalog: Apoptosis
  • Density: 1.19
  • Boiling Point: 726.4±60.0 °C at 760 mmHg
  • Melting Point: 225 °C
  • Flash Point: 393.1±32.9 °C

RXP470.1

RXP470.1 (RXP-470) is a potent, selective MMP-12 inhibitor with a Ki of 0.2 nM against human MMP-12. RXP470.1 is 2 to 4 orders of magnitude less potent against other MMPs. RXP470.1 significantly reduces atherosclerotic plaque cross-sectional area in mouse. RXP470.1 results in less complex plaques with increased smooth muscle cell:macrophage ratio, less macrophage apoptosis, increased cap thickness, smaller necrotic cores, and decreased incidence of calcification[1].

  • CAS Number: 891198-31-5
  • MF: C35H35BrClN4O10P
  • MW: 818.00
  • Catalog: MMP
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

HIV Protease Substrate 1 TFA

HIV Protease Substrate 1, a fiuorogenic HIV protease substrate, can be used to study enzymatic activity of HIV protease[1].

  • CAS Number: 223769-59-3
  • MF: C92H133N27O23S
  • MW: 2017.27
  • Catalog: HIV Protease
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Nidufexor

Nidufexor is a farnesoid X receptor (FXR) agonist[1].

  • CAS Number: 1773489-72-7
  • MF: C27H22ClN3O4
  • MW: 487.93
  • Catalog: FXR
  • Density: 1.4±0.1 g/cm3
  • Boiling Point: 781.1±60.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 426.2±32.9 °C

ginsenoside Rd

Ginsenoside Rd inhibits TNFα-induced NF-κB transcriptional activity with an IC50 of 12.05±0.82 μM in HepG2 cells. Ginsenoside Rd inhibits expression of COX-2 and iNOS mRNA. Ginsenoside Rd also inhibits Ca2+ influx. Ginsenoside Rd inhibits CYP2D6, CYP1A2, CYP3A4, and CYP2C9, with IC50s of 58.0±4.5 μM, 78.4±5.3 μM, 81.7±2.6 μM, and 85.1±9.1 μM, respectively.

  • CAS Number: 52705-93-8
  • MF: C48H82O18
  • MW: 947.154
  • Catalog: Calcium Channel
  • Density: 1.4±0.1 g/cm3
  • Boiling Point: 1015.6±65.0 °C at 760 mmHg
  • Melting Point: 207 °C(dec.)
  • Flash Point: 568.0±34.3 °C

Bz-Tyr-pNA

N-Benzoyl-L-tyrosine p-nitroanilide is a chymotrypsin substrate[1].

  • CAS Number: 6154-45-6
  • MF: C22H19N3O5
  • MW: 405.40
  • Catalog: Ser/Thr Protease
  • Density: 1.373 g/cm3
  • Boiling Point: 776.8ºC at 760 mmHg
  • Melting Point: 235-237 °C
  • Flash Point: 423.6ºC

Brecanavir

Brecanavir (GW640385) is a novel, potent HIV protease inhibitor.

  • CAS Number: 313682-08-5
  • MF: C33H41N3O10S2
  • MW: 703.82300
  • Catalog: HIV Protease
  • Density: 1.43g/cm3
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Fluindione

Fluindione is a inhibitor of 5-lipoxygenase with the IC50 of 15 μM. Fluindione has antiinflammatory activity[1].

  • CAS Number: 957-56-2
  • MF: C15H9FO2
  • MW: 240.23
  • Catalog: 5-Lipoxygenase
  • Density: 1.335g/cm3
  • Boiling Point: 406.3ºC at 760mmHg
  • Melting Point: N/A
  • Flash Point: 154.5ºC

Carbonic anhydrase inhibitor 14

Carbonic anhydrase inhibitor 14 (Compound 8b) is a carbonic anhydrase (CA) inhibitor with Ki values of 1203, 99.7, 9.4 and 27.7 nM against hCA I, hCA II, hCA IX and hCA XII, respectively. Carbonic anhydrase inhibitor 14 can also inhibit CDK2 with an IC50 of 20.3 μM. Carbonic anhydrase inhibitor 14 shows antitumor activity[1].

  • CAS Number: 2410402-75-2
  • MF: C18H17N7O2S
  • MW: 395.44
  • Catalog: CDK
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

3",8"-Binaringenin

Tetrahydroamentoflavone (Amentoflavanone) is a potent xanthine oxidase (XO) inhibitor. Tetrahydroamentoflavone has inhibitory activity for XO with IC50 and Ki values of 92 nM and 0.982 μM, respectively. Tetrahydroamentoflavone can be used for the research of inflammatory disorders and gout[1].

  • CAS Number: 48236-96-0
  • MF: C30H22O10
  • MW: 542.490
  • Catalog: Xanthine Oxidase
  • Density: 1.6±0.1 g/cm3
  • Boiling Point: 932.8±65.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 314.3±27.8 °C

Benzthiazide

Benzthiazide is a long-acting diuretic[1] and a hypertension agent. Benzthiazide is an inhibitor of carbonic anhydrase 9 (CA9), with Kis of 8.0, 8.8 and 10 nM for CA9, CA2 and CA1, respectively. Benzthiazide also suppresses proliferation of cancer cells[2].

  • CAS Number: 91-33-8
  • MF: C15H14ClN3O4S3
  • MW: 431.93700
  • Catalog: Carbonic Anhydrase
  • Density: 1.4176 (rough estimate)
  • Boiling Point: 680.6 °C at 760 mmHg
  • Melting Point: 231-232° (U.S. patent); mp 238-239° (P'an)
  • Flash Point: 365.4 °C

Phospholipase D

Phospholipase D (PLD) is an enzyme of the phospholipase superfamily, which widely exists in bacteria, yeast, plants, animals and viruses, and is often used in biochemical research. Phospholipase D can catalyze the hydrolysis of phosphodiester bonds in glycerophospholipids to produce phosphatidic acid and soluble choline. Phospholipase D is involved in a variety of disease-related processes, including diabetes, atherogenesis, obesity, tumorigenesis, immune response, and neuroendocrine function[1].

  • CAS Number: 9001-87-0
  • MF: C9H14N4O3
  • MW: 226.232
  • Catalog: Phospholipase
  • Density: 1.4±0.1 g/cm3
  • Boiling Point: 656.2±55.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 350.7±31.5 °C

ATX inhibitor 1

ATX inhibitor 1 is a potent ATX (IC50=1.23 nM, FS-3 and 2.18 nM, bis-pNPP) inhibitor.

  • CAS Number: 2225892-70-4
  • MF: C21H23Cl2N2O6P
  • MW: 501.30
  • Catalog: Phosphodiesterase (PDE)
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

BMS-309403

BMS-309403 is a potent, selective and cell-permeable inhibitor of fatty acid binding protein 4 (FABP4) with a Ki of less than 2 nM.

  • CAS Number: 300657-03-8
  • MF: C31H26N2O3
  • MW: 474.550
  • Catalog: FABP
  • Density: 1.2±0.1 g/cm3
  • Boiling Point: 657.5±55.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 351.4±31.5 °C

NAMPT/IDO1-IN-1

NAMPT/IDO1-IN-1 is an orally active dual inhibitor of NAMPT and IDO1 with IC50s of 57.7 nM and 233 nM, respectively. NAMPT/IDO1-IN-1 blocks NAD+ biosynthesis, inhibits proliferation and migration of Paclitaxel (HY-B0015)- and FK866 (HY-50876)-resistant NSCLC cell lines (A549/R cells). NAMPT/IDO1-IN-1 has shown antitumor effects in mice and enhanced A549/R cell sensitivity to paclitaxel[1].

  • CAS Number: 2247884-06-4
  • MF: C23H24BrFN8O3
  • MW: 559.39
  • Catalog: Indoleamine 2,3-Dioxygenase (IDO)
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

MitoTam bromide, hydrobromide

MitoTam bromide, hydrobromide is a tamoxifen derivative[1], an electron transport chain (ETC) inhibitor, spreduces mitochondrial membrane potential in senescent cells and affects mitochondrial morphology[2].MitoTam bromide, hydrobromide is an effective anticancer agent, suppresses respiratory complexes (CI-respiration) and disrupts respiratory supercomplexes (SCs) formation in breast cancer cells[1][2]. MitoTam bromide, hydrobromide causes apoptosis[2].

  • CAS Number: 1634624-73-9
  • MF: C52H60Br2NOP
  • MW: 905.82
  • Catalog: Apoptosis
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Vildagliptin dihydrate

Vildagliptin (LAF237 dihydrate;NVP-LAF 237 dihydrate) is a dipeptidyl peptidase 4 (DPP4) inhibitor that delays the degradation of glucagon-like peptide-1 (GLP-1).

  • CAS Number: 2133364-01-7
  • MF: C17H29N3O4
  • MW: 339.43
  • Catalog: Dipeptidyl Peptidase
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

6-epi-Tadalafil

cis-ent-Tadalafil (Tadalafil EP Impurity C) is a highly potent PDE5 inhibitor with an IC50 value of 5 nM and has blood pressure lowering activity[1].

  • CAS Number: 171596-28-4
  • MF: C22H19N3O4
  • MW: 389.40
  • Catalog: Phosphodiesterase (PDE)
  • Density: 1.51±0.1 g/cm3 (20 ºC 760 Torr)
  • Boiling Point: N/A
  • Melting Point: 290-292 ºC
  • Flash Point: N/A

BODIPY aminoacetaldehyde

BODIPY aminoacetaldehyde (BAAA) is a fluorescent substrate for both murine and human aldehyde dehydrogenase (ALDH). BODIPY aminoacetaldehyde consists of an aminoacetaldehyde moiety bonded to the BODIPY fluorochrome and can be used to label stem cells[1].

  • CAS Number: 247069-94-9
  • MF: C16H18BF2N3O2
  • MW: 333.14
  • Catalog: Aldehyde Dehydrogenase (ALDH)
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

ML368

SR9186 (ML368) is a potent CYP3A4 inhibitor with IC50 s for inhibition of midazolam → 1′hydroxymidazolam, testosterone → 6β-hydroxytestosterone, and vincristine → vincristine M1 of 9, 4, and 38 nM, respectively. SR-9186 inhibits liver-stage development of P. falciparum to block ivermectin metabolism[1].

  • CAS Number: 1361414-26-7
  • MF: C26H18N6O
  • MW: 430.46100
  • Catalog: Cytochrome P450
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

CA-074 methyl ester

CA-074 methyl ester is a specific inhibitor of Cathepsin B, which has potent bioactivities such as neuroprotective, anti-cancer, and anti-inflamatory effects.

  • CAS Number: 147859-80-1
  • MF: C19H31N3O6
  • MW: 397.46600
  • Catalog: Cathepsin
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

FPL 62064

FPL 62064 is a potent 5-lipoxygenase (5-LOX) and COX dual inhibitor, with IC50 values of 3.5 μM and 3.1 μM for RBL-1 cytosolic 5-lipoxygenase and prostaglandin synthetase (cyclooxygenase), respectively. FPL 62064 has potent anti-inflammatory activity[1][2].

  • CAS Number: 103141-09-9
  • MF: C16H15N3O
  • MW: 265.310
  • Catalog: COX
  • Density: 1.2±0.1 g/cm3
  • Boiling Point: 442.6±25.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 221.5±23.2 °C

hCAXII-IN-5

hCAXII-IN-5 (compound 6o) is a potent and selective hCAXII inhibitor with Ki values of >10000, >10000, 286.1, 1.0 nM for hCAI, hCAII, hCAIX and hCAXII, respectively[1].

  • CAS Number: 2414629-92-6
  • MF: C23H22N2O4
  • MW: 390.43
  • Catalog: Carbonic Anhydrase
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

PHD2-IN-1

PHD2-IN-1 is a potent and orally active inhibitor of HIF prolyl hydroxylase 2 (PHD2) with an IC50 of 22.53 nM. PHD2-IN-1 can be used for anemia research[1].

  • CAS Number: 2768219-28-7
  • MF: C21H23ClN4O5
  • MW: 446.88
  • Catalog: HIF/HIF Prolyl-Hydroxylase
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

A-69412

A-69412 is a reversible, specific inhibitor of the hydrophilic 5-lipoxygenase (5-LO).

  • CAS Number: 123606-23-5
  • MF: C7H10N2O3
  • MW: 170.16600
  • Catalog: 5-Lipoxygenase
  • Density: 1.33g/cm3
  • Boiling Point: 325.2ºC at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 150.5ºC

FTI 276 TFA

FTI-276 is a protein farnesyl transferase (PFT) inhibitor with IC50s of 0.9 nM and 0.5 nM for Plasmodium falciparum and human, respectively[1].

  • CAS Number: 1217471-51-6
  • MF: C23H28F3N3O5S2
  • MW: 547.61
  • Catalog: Farnesyl Transferase
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

SEP-0372814

PDE10-IN-2 is a PDE10 inhibitor with an IC50≦0.01 μM[1].

  • CAS Number: 1516895-53-6
  • MF: C21H19N7
  • MW: 369.42246
  • Catalog: Phosphodiesterase (PDE)
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

MK 386

MK 386 (L-733692) is a selective 5-α-reductase I inhibitor, used for prostate cancer inhibition[1].

  • CAS Number: 158493-17-5
  • MF: C28H49NO
  • MW: 415.69
  • Catalog: 5 alpha Reductase
  • Density: 0.951g/cm3
  • Boiling Point: 505.2ºC at 760mmHg
  • Melting Point: N/A
  • Flash Point: 203.9ºC

Epoxomicin

Epoxomicin is a cell-permeable and irreversible proteasome inhibitor, primarily the chymotrypsin-like activity.

  • CAS Number: 134381-21-8
  • MF: C28H50N4O7
  • MW: 554.719
  • Catalog: Proteasome
  • Density: 1.1±0.1 g/cm3
  • Boiling Point: 795.7±60.0 °C at 760 mmHg
  • Melting Point: 107-109ºC
  • Flash Point: 435.0±32.9 °C

Fasidotril

Fasidotril is a dual inhibitor of neprilysin and angiotensin-converting enzyme (ACE) for the potential treatment of hypertension and congestive heart failure (CHF).

  • CAS Number: 135038-57-2
  • MF: C23H25NO6S
  • MW: 443.51300
  • Catalog: Angiotensin-converting Enzyme (ACE)
  • Density: 1.276g/cm3
  • Boiling Point: 625.3ºC at 760mmHg
  • Melting Point: N/A
  • Flash Point: 332ºC