Antifolates are compounds that antagonise the actions of folic acid (vitamin B9). Folic acid's primary function in the body is as a cofactor to various methyltransferases involved in serine, methionine, thymidine and purine biosynthesis. Consequently antifolates inhibit cell division, DNA/RNA synthesis and repair and protein synthesis. Some such as Proguanil, Pyrimethamine and Trimethoprim selectively inhibit folate's actions in microbial organisms such as bacteria, protozoa and fungi. The majority of antifolates work by inhibiting dihydrofolate reductase (DHFR). Many are primarily DHFR inhibitors, but Raltitrexed is an inhibitor of thymidylate synthase, and Pemetrexed inhibits both and a third enzyme. Antifolates act specifically during DNA and RNA synthesis, and thus are cytotoxic during the S-phase of the cell cycle. Thus, they have a greater toxic effect on rapidly dividing cells.


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Levomefolic acid

Levomefolic acid (5-MTHF) is the natural, active form of folic acid used at the cellular level for DNA reproduction, the cysteine cycle and the regulation of homocysteine among other functions. IC50 value: Target: Folate analogLevomefolic acid has been proposed for treatment of cardiovascular disease and advanced cancers such as breast and colorectal cancers. Levomefolic acid (5-MTHF) has the prominent antioxidant activity. A high dose of 5-MTHF or folic acid does not influence Natural killer (NK) cell function in vitro.

  • CAS Number: 31690-09-2
  • MF: C20H25N7O6
  • MW: 459.456
  • Catalog: Antifolate
  • Density: 1.6±0.1 g/cm3
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Pemetrexed

Pemetrexed disodium is a novel antifolate that inhibits the folatedependent enzymes thymidylate synthase, dihydrofolate reductase, and glycinamide ribonucleotide formyltransferase with Kis of 1.3, 7.2, and 65 nM, respectively.

  • CAS Number: 150399-23-8
  • MF: C20H19N5Na2O6
  • MW: 471.374
  • Catalog: Autophagy
  • Density: N/A
  • Boiling Point: 160°C 20mm
  • Melting Point: 36-38°C
  • Flash Point: 160°C/20mm

sulfuric acid,5-[(3,4,5-trimethoxyphenyl)methyl]pyrimidine-2,4-diamine

Trimethoprim sulfate is a bacteriostatic antibiotic and an orally active dihydrofolate reductase inhibitor. Trimethoprim sulfate is active against a wide range of Gram-positive and Gram-negative aerobic bacteria. Trimethoprim sulfate has the potential for the research of urinary tract infections, Shigellosis and Pneumocystis pneumonia. Trimethoprim sulfate can inhibit infection of Influenza A virus in chick embryo when combinated with zinc[1][2][3][4].

  • CAS Number: 56585-33-2
  • MF: C28H38N8O10S
  • MW: 678.71400
  • Catalog: Bacterial
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Pemetrexed-d5

Pemetrexed-d5 is the deuterium labeled Pemetrexed[1]. Pemetrexed (LY231514) is an antifolate, the Ki values of the pentaglutamate of Pemetrexed (LY231514) are 1.3, 7.2, and 65 nM for inhibits thymidylate synthase (TS), dihydrofolate reductase (DHFR), and glycinamide ribonucleotide formyltransferase (GARFT), respectively[2].

  • CAS Number: 1129408-57-6
  • MF: C20H16D5N5O6
  • MW: 432.44
  • Catalog: Autophagy
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

FRα-IN-1

FRα-IN-1 (Compound 4) is a tumor-targeting agent. FRα-IN-1 shows selective anticancer activity towards folate receptors (FRα and FRβ) expression cells[1].

  • CAS Number: 2028296-64-0
  • MF: C21H24N6O6
  • MW: 456.45
  • Catalog: Antifolate
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Fanotaprim

Fanotaprim is a dihydrofolate reductase (DHFR) inhibitor with IC50s of 1.57 and 308 nM for tgDHFR (Toxoplasma gondii DHFR) and hDHFR (human DHFR), respectively. Fanotaprim has the potential for the research of toxoplasmosis[1].

  • CAS Number: 2120282-75-7
  • MF: C19H22N8O
  • MW: 378.43
  • Catalog: Antifolate
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Edatrexate

Edatrexate (CGP 30694), as known as 10-Ethyl-10-deazaaminopterin, is Methotrexate (HY-14519) analog, exhibits antitumor activity against MTX-resistant tumors. Edatrexate is an antifolate antimetabolite, can be used for reasearch of non-small-cell lung cancer, breast cancer, non-Hodgkin's lymphoma, and cancer of the head and neck[1][2].

  • CAS Number: 80576-83-6
  • MF: C22H25N7O5
  • MW: 467.47800
  • Catalog: Antifolate
  • Density: 1.441 g/cm3
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Pemetrexed disodium

Pemetrexed is a novel antifolate, the Ki values of the pentaglutamate of LY231514 are 1.3, 7.2, and 65 nM for inhibits thymidylate synthase (TS), dihydrofolate reductase (DHFR), and glycinamide ribonucleotide formyltransferase (GARFT), respectively.

  • CAS Number: 137281-23-3
  • MF: C20H21N5O6
  • MW: 427.411
  • Catalog: Autophagy
  • Density: 1.6±0.1 g/cm3
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Benzoic acid,2-[[[(4S)-4-carboxy-4-[[4-[[(2,4-diamino-6-pteridinyl)methyl]amino]benzoyl]amino]butyl]amino]carbonyl]-

Talotrexin (PT523), an analog of Aminopterin (HY-14518), is a nonpolyglutamatable classic antifolate. Talotrexin is a RFC (reduced folate carrier) specific inhibitor and selectively inhibits RFC transport. Talotrexin shows antitumor activity by targeting DHFR to inhibit tumor growth[1][2].

  • CAS Number: 113857-87-7
  • MF: C27H27N9O6
  • MW: 573.56000
  • Catalog: Antifolate
  • Density: 1.51g/cm3
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Methotrexate-d3

Methotrexate-d3 (Amethopterin-d3) is the deuterium labeled Methotrexate. Methotrexate (Amethopterin), an antimetabolite and antifolate agent, inhibits the enzyme dihydrofolate reductase, thereby preventing the conversion of folic acid into tetrahydrofolate, and inhibiting DNA synthesis. Methotrexate, also an immunosuppressant and antineoplastic agent, is used for the research of rheumatoid arthritis and a number of different cancers (such as acute lymphoblastic leukemia)[1][2][3].

  • CAS Number: 432545-63-6
  • MF: C20H19D3N8O5
  • MW: 457.45800
  • Catalog: ADC Cytotoxin
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: 210°C dec.
  • Flash Point: 9℃

PeMetrexed DisodiuM Heptahydrate

Pemetrexed disodium heptahydrate is a novel antifolate, the Ki values of the pentaglutamate of LY231514 are 1.3, 7.2, and 65 nM for inhibits thymidylate synthase (TS), dihydrofolate reductase (DHFR), and glycinamide ribonucleotide formyltransferase (GARFT), respectively.

  • CAS Number: 357166-29-1
  • MF: C20H33N5Na2O13
  • MW: 597.481
  • Catalog: Autophagy
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Methotrexate disodium

Methotrexate (Amethopterin) disodium, an antimetabolite and antifolate agent, inhibits the enzyme dihydrofolate reductase, thereby preventing the conversion of folic acid into tetrahydrofolate, and inhibiting DNA synthesis. Methotrexate disodium, also an immunosuppressant and antineoplastic agent, is used for the research of rheumatoid arthritis and a number of different cancers (such as acute lymphoblastic leukemia)[1][2][3].

  • CAS Number: 7413-34-5
  • MF: C20H20N8Na2O5
  • MW: 498.403
  • Catalog: ADC Cytotoxin
  • Density: N/A
  • Boiling Point: 823℃
  • Melting Point: N/A
  • Flash Point: N/A

Tetroxoprim

Tetroxoprim is an antimicrobial DHFR inhibitor[1].

  • CAS Number: 53808-87-0
  • MF: C16H22N4O4
  • MW: 334.37
  • Catalog: Antifolate
  • Density: 1.232g/cm3
  • Boiling Point: 564.1ºC at 760mmHg
  • Melting Point: N/A
  • Flash Point: 294.9ºC

10-formylfolic acid (25 mg)

10-Formylfolic acid is a potent inhibitor of dihydrofolate reductase. 10-Formylfolic acid be used as an early indicator of leukemia[1][2].

  • CAS Number: 134-05-4
  • MF: C20H19N7O7
  • MW: 469.40800
  • Catalog: Antifolate
  • Density: 1.67g/cm3
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

CI-898 HCl

Trimetrexate trihydrochloride is a antibiotic, also a potent dihydrofolate reductase (DHFR) inhibitor, reducing the production of DNA and RNA precursors and leading to cell death, with IC50 values of 4.74 nM and 1.35 nM for human DHFR and Toxoplasma gondii DHFR. Trimetrexate trihydrochloride can be used for researching Pneumocystis carinii pneumonia (PCP)[1][2].

  • CAS Number: 1658520-97-8
  • MF: C19H26Cl3N5O3
  • MW: 478.800
  • Catalog: Bacterial
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Levomefolate calcium

Levomefolate is an artificial form of folate.IC50 Value:Target: AntifolateThe calcium salt of L-5-methyltetrahydrofolic acid which belongs to the group of folate vitamins (Vitamin B9, Folacin). It is a coenzymated form of folic acid and a more bioavailable alternative in dietary supplements.

  • CAS Number: 151533-22-1
  • MF: C20H23CaN7O6
  • MW: 497.518
  • Catalog: Antifolate
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: >300ºC
  • Flash Point: N/A

Methotrexate

Methotrexate is a folate antagonist, with median IC50 of 78 nM in in vitro assay.

  • CAS Number: 59-05-2
  • MF: C20H22N8O5
  • MW: 454.439
  • Catalog: ADC Cytotoxin
  • Density: 1.4080
  • Boiling Point: 561.26°C
  • Melting Point: 195°C
  • Flash Point: 11℃

Calcium N5-methyltetrahydrofolate

Calcium N5-methyltetrahydrofolate(NSC173328) is the calcium salt of levomefolic acid, which has been proposed for treatment of cardiovascular disease and advanced cancers such as breast and colorectal cancers.IC50 value:Target:

  • CAS Number: 26560-38-3
  • MF: C20H23CaN7O6
  • MW: 497.52
  • Catalog: Antifolate
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

LY 254155

LY 254155, an antifolate, inhibits hGARFT and binds to mFBP with Kis of 2.1±0.2 and 1.7±0.1 nM, respectively.

  • CAS Number: 135503-67-2
  • MF: C19H23N5O6S
  • MW: 449.48100
  • Catalog: Antifolate
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Lometrexol disodium

Lometrexol (DDATHF) disodium, an antipurine antifolate, can inhibit the activity of glycinamide ribonucleotide formyltransferase (GARFT) but do not induce detectable levels of DNA strand breaks. Lometrexol disodium can further inhibit de novo purine synthesis, causing abnormal cell proliferation and apoptosis, even cell cycle arrest. Lometrexol disodium has anticancer activity. Lometrexol disodium also is a potent human Serine hydroxymethyltransferase1/2 (hSHMT1/2) inhibitor[1][2][3].

  • CAS Number: 120408-07-3
  • MF: C21H25N5NaO6+
  • MW: 487.417
  • Catalog: Apoptosis
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Folinic acid calcium hydrate

Folinic acid (Leucovorin) calcium hydrate is a biological folic acid and is generally administered along with Methotrexate (MTX) as a rescue agent to decrease MTX-induced toxicity[1].

  • CAS Number: 1097832-14-8
  • MF: C20H25CaN7O8
  • MW: 531.53
  • Catalog: Antifolate
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Lometrexol

Lometrexol (DDATHF), an antipurine antifolate, can inhibit the activity of glycinamide ribonucleotide formyltransferase (GARFT) by tightly binding with it. Lometrexol can further inhibit de novo purine synthesis, causing abnormal cell proliferation and apoptosis, even cell cycle arrest. Lometrexol has anticancer activity[1].

  • CAS Number: 106400-81-1
  • MF: C21H25N5O6
  • MW: 443.45300
  • Catalog: Apoptosis
  • Density: 1.56g/cm3
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

LY309887

LY309887 is a potent inhibitor of glycinamide ribonucleotide formyltransferase (GARFT), with a Ki of 6.5 nM, and has antitumor activity.

  • CAS Number: 127228-54-0
  • MF: C19H23N5O6S
  • MW: 449.48100
  • Catalog: Antifolate
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

D-AMETHOPTERIN HYDRATE

Methotrexate (Amethopterin) monohydrate, an antimetabolite and antifolate agent, inhibits the enzyme dihydrofolate reductase, thereby preventing the conversion of folic acid into tetrahydrofolate, and inhibiting DNA synthesis. Methotrexate monohydrate, also an immunosuppressant and antineoplastic agent, is used for the research of rheumatoid arthritis and a number of different cancers (such as acute lymphoblastic leukemia)[1][2][3].

  • CAS Number: 6745-93-3
  • MF: C20H24N8O6
  • MW: 472.45500
  • Catalog: ADC Cytotoxin
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: 195ºC (dec.)(lit.)
  • Flash Point: N/A

Diaveridine

Diaveridine (EGIS-5645) is a dihydrofolate reductase (DHFR) inhibitor with a Ki of 11.5 nM for the wild type DHFR and also an antibacterial agent.

  • CAS Number: 5355-16-8
  • MF: C13H16N4O2
  • MW: 260.292
  • Catalog: Bacterial
  • Density: 1.3±0.1 g/cm3
  • Boiling Point: 506.1±60.0 °C at 760 mmHg
  • Melting Point: 232 - 236ºC
  • Flash Point: 259.9±32.9 °C

Aminopterin

Aminopterin (4-Aminofolic acid), the 4-amino derivative of folic acid, is a folic acid antagonist. Aminopterin catalyses the reduction of folic acid to tetrahydrofolic acid, and competitively inhibits dihydrofolate reductase (DHFR) with a Ki of 3.7 pM. Aminopterin has anticancer and immunosuppressive activity. Aminopterin is used in treatment of pediatric leukemia[1][2].

  • CAS Number: 54-62-6
  • MF: C19H20N8O5
  • MW: 440.413
  • Catalog: Antifolate
  • Density: 1.6±0.1 g/cm3
  • Boiling Point: N/A
  • Melting Point: 228-235 °C (dec.)
  • Flash Point: N/A

Lysine-methotrexate

Lysine-methotrexate, an analogue of Methotrexate (HY-14519), is a dihydrofolate reductase (DHFR) inhibitor[1].

  • CAS Number: 80407-56-3
  • MF: C21H27N9O3
  • MW: 453.49800
  • Catalog: Antifolate
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A