Cell apoptosis, sometimes called programmed cell death, is a cellular self-destruction method to remove old and damaged cells during development and aging to protect cells from external disturbances and maintain homeostasis. Apoptosis also occurs as a defense mechanism such as in immune reactions or when cells are damaged by disease or noxious agents.

Apoptosis is controlled by many genes and involves two fundamental pathways: the extrinsic pathway, which transmits death signals by the death receptor (DR), and the intrinsic or mitochondrial pathway. The extrinsic apoptotic pathway is activated by the binding of the death ligand to DRs, including FasL, TNF-α, and TRAIL, on the plasma membrane. The DR, adaptor protein (FADD), and associated apoptosis signaling molecule (caspase-8) form the death-inducing signaling complex (DISC), thus leading to the activation of the effector caspase cascade (caspase-3, -6, and -7). The mitochondria-mediated intrinsic apoptosis pathway is regulated by Bcl-2 family proteins, including proapoptotic (Bid, Bax, Bak) and antiapoptotic proteins (Bcl-2, Bcl-xL).

Abnormalities in cell apoptosis can be a significant component of diseases such as cancer, autoimmune lymphoproliferative syndrome, AIDS, ischemia, and neurode-generative diseases. These diseases may benefit from artificially inhibiting or activating apoptosis. A short list of potential methods of anti-apoptotic therapy includes stimulation of the IAP (inhibitors of apoptosis proteins) family of proteins, caspase inhibition, PARP (poly [ADP-ribose] polymerase) inhibition, stimulation of the PKB/Akt (protein kinase B) pathway, and inhibition of Bcl-2 proteins.

Ferroptosis and necroptosis are recently recognized forms of regulated cell death that differs considerably from apoptosis. Misregulated ferroptosis or necroptosis have also been implicated in multiple physiological and pathological processes, including cancer cell death, neurotoxicity, neurodegenerative diseases, etc.

References:
[1] Susan Elmore. Toxicol Pathol. 2007; 35(4): 495–516.
[2] Cao L, et al. J Cell Death. 2016 Dec 29;9:19-29.
[3] Dasgupta A, et al. Int J Mol Sci. 2017 Jan; 18(1): 23.
[4] Xie Y, et al. Cell Death Differ. 2016 Mar;23(3):369-79.


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Epibrassinolide

Epibrassinolide is a natural brassinosteroid (BR) derivative, is a plant regulator with a similar structure to mammalian steroids. Epibrassinolide is a potential apoptotic inducer in various cancer cells without affecting the non-tumor cell growth.

  • CAS Number: 78821-43-9
  • MF: C28H48O6
  • MW: 480.677
  • Catalog: Apoptosis
  • Density: 1.1±0.1 g/cm3
  • Boiling Point: 633.7±55.0 °C at 760 mmHg
  • Melting Point: 256°C
  • Flash Point: 202.3±25.0 °C

Neriifolin

Neriifolin, a CNS-penetrating cardiac glycoside, is an inhibitor of the Na+, K+-ATPase. Neriifolin can target beclin 1, inhibits the formation of LC3-associated phagosomes and ameliorates experimental autoimmune encephalomyelitis (EAE) development. Neriifolin induces cell cycle arrest and apoptosis in human hepatocellular carcinoma HepG2 cells[1][2.

  • CAS Number: 466-07-9
  • MF: C30H46O8
  • MW: 534.68100
  • Catalog: Apoptosis
  • Density: 1.274g/cm3
  • Boiling Point: 700.079ºC at 760 mmHg
  • Melting Point: 218-225ºC
  • Flash Point: 224.862ºC

Trifluorothymidine

Trifluridine is an irreversible thymidylate synthase inhibitor, and thereby suppresses DNA synthesis. Trifluridine is an antiviral drug for herpes simplex virus (HSV) infection.

  • CAS Number: 70-00-8
  • MF: C10H11F3N2O5
  • MW: 296.200
  • Catalog: Thymidylate Synthase
  • Density: 1.6±0.1 g/cm3
  • Boiling Point: N/A
  • Melting Point: 190-193 °C(lit.)
  • Flash Point: N/A

GPX4-IN-6

GPX4-IN-6 (Compound C25) is a GPX4 covalent inhibitor with an IC50 value of 0.13 μM. GPX4-IN-6 (Compound C25) can induce ferroptosis for the research of triple-negative breast cancer (TNBC) [1].

  • CAS Number: 2922824-07-3
  • MF: C18H17BrFNO5
  • MW: 426.23
  • Catalog: Ferroptosis
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

RIPK2-IN-2

RIPK2-IN-2 (example 25) is a RIP2 kinase PROTAC inhibitor. RIPK2-IN-2 can block RIP2-dependent proinflammatory signaling, regulated RIP2 kinase activity in auto inflammatory diseases[1].

  • CAS Number: 2143956-20-9
  • MF: C53H65FN14O7S2
  • MW: 1093.30
  • Catalog: RIP kinase
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

p53 and MDM2 proteins-interaction-inhibitor (racemic)

p53 and MDM2 proteins-interaction-inhibitor (racemic) (Compound 2j) is an inhibitor of the interaction between p53 and MDM2 proteins.

  • CAS Number: 939983-14-9
  • MF: C40H49Cl2N5O4
  • MW: 734.75400
  • Catalog: MDM-2/p53
  • Density: 1.24
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

BCL2-IN-1

BCL2-IN-1 is a potent Bcl-2 inhibitor. BCL2-IN-1 binds Bcl-2 with a Ki of <0.01 nM[1].

  • CAS Number: 1257044-75-9
  • MF: C47H54ClN7O7S
  • MW: 896.49
  • Catalog: Bcl-2 Family
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

S63845

S63845 is a potent and selective myeloid cell leukemia 1 (MCL1) inhibitor with a Kd of 0.19 nM for human MCL1.

  • CAS Number: 1799633-27-4
  • MF: C39H37ClF4N6O6S
  • MW: 829.26
  • Catalog: Bcl-2 Family
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

JKE-1674

JKE-1674 is an orally active glutathione peroxidase 4 (GPX4) inhibitor. JKE-1674 kills cells in a manner that is equipotent to ML210 and is completely rescued by ferroptosis inhibitors[1].

  • CAS Number: 2421119-60-8
  • MF: C20H20Cl2N4O4
  • MW: 451.303
  • Catalog: Ferroptosis
  • Density: 1.4±0.1 g/cm3
  • Boiling Point: 595.5±60.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 313.9±32.9 °C

GSK621

GSK621 is a specific AMPK activator, with IC50 values of 13-30 μM for AML cells. GSK621 induces autophagy and apoptosis. GSK621 induces eiF2α phosphorylation-a hallmark of UPR activation[1].

  • CAS Number: 1346607-05-3
  • MF: C26H20ClN3O5
  • MW: 489.907
  • Catalog: Apoptosis
  • Density: 1.4±0.1 g/cm3
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

SB 202190 hydrochloride

SB 202190 hydrochloride is a selective p38 MAP kinase inhibitor with IC50s of 50 nM and 100 nM for p38α and p38β2, respectively. SB 202190 hydrochloride binds to the ATP pocket of the active recombinant human p38 kinase with a Kd of 38 nM. SB 202190 hydrochloride has anti-cancer activity[1][2]. SB202190 hydrochloride induces autophagy[3].

  • CAS Number: 350228-36-3
  • MF: C20H15ClFN3O
  • MW: 367.80
  • Catalog: Apoptosis
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Rotundifuran

Rotundifuran, a labdane type diterpene, is isolated from Vitex rotundifolia. Rotundifuran can inhibit the cell cycle progression and induce apoptosis in human myeloid leukaemia cells[1][2].

  • CAS Number: 50656-65-0
  • MF: C22H34O4
  • MW: 362.50300
  • Catalog: Apoptosis
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

ASK1-IN-4

ASK1-IN-4 (Compound 17) is an ASK1 inhibitor (IC50=0.2 μM). ASK1-IN-4 interacts with ATP-binding site of ASK1[1].

  • CAS Number: 1427538-26-8
  • MF: C18H14BrNO4S2
  • MW: 452.34
  • Catalog: Apoptosis
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

DB818

DB818 (DB-818, DB 818) is a small molecule inhibitor of HOXA9/DNA interaction through binding as minor groove DNA ligand on the HOXA9 cognate sequence; directly bind to HOXA9-cognate sequence with Kd value of 4.6 nM (HBS sequence); alters HOXA9-mediated transcription, cell survival and cell cycle inHOXA9-mediated HOXA9-expressing murine MigA9 cell line.

  • CAS Number: 790241-43-9
  • MF: C19H16N6S
  • MW: 360.439
  • Catalog: Apoptosis
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

o-Methoxycinnamaldehyde

2-Methoxycinnamaldehyde (o-Methoxycinnamaldehyde) is a natural compound of Cinnamomum cassia, with antitumor activity[1][2][3]. 2-Methoxycinnamaldehyde inhibits proliferation and induces apoptosis by mitochondrial membrane potential (ΔΨm) loss, activation of both caspase-3 and caspase-9[2]. 2-Methoxycinnamaldehyde effectively inhibits platelet-derived growth factor (PDGF)-induced HASMC migration[3].

  • CAS Number: 1504-74-1
  • MF: C10H10O2
  • MW: 162.185
  • Catalog: Apoptosis
  • Density: 1.1±0.1 g/cm3
  • Boiling Point: 334.8±0.0 °C at 760 mmHg
  • Melting Point: 44-48ºC
  • Flash Point: 134.4±13.9 °C

pinusolide

Pinusolide is a known platelet-activating factor (PAF) receptor binding antagonist. Pinusolide not only decreases the proliferation activity of tumor cells but specifically induces apoptosis[1].

  • CAS Number: 31685-80-0
  • MF: C21H30O4
  • MW: 346.461
  • Catalog: Apoptosis
  • Density: 1.1±0.1 g/cm3
  • Boiling Point: 465.3±38.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 228.5±25.2 °C

Mca-Tyr-Val-Ala-Asp-Ala-Pro-Lys(Dnp)-OH trifluoroacetate salt

Mca-YVADAP-Lys(Dnp)-OH is a fluorogenic substrate for caspase-1 and angiotensin-converting enzyme 2 (ACE2)[1].

  • CAS Number: 189696-01-3
  • MF: C53H64N10O19
  • MW: 1145.13000
  • Catalog: Caspase
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

MDM2-IN-1

MDM2-IN-1 (Compound 30) is a synthetic MDM2-p53 interaction (MDM2) inhibitor and contains the trans (D-)configuration[1].

  • CAS Number: 1410737-09-5
  • MF: C23H21Cl2FN2O3
  • MW: 463.33
  • Catalog: MDM-2/p53
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Phellamurin

Phellamurin is a plant flavonone glycoside from the leaves of Phellodendron amurense and inhibits intestinal P-glycoprotein. Phellamurin also inhibits egg laying by Papilio protenor. Phellamurin induces cells apoptosis and has anti-tumor activity[1][2][3].

  • CAS Number: 52589-11-4
  • MF: C26H30O11
  • MW: 518.510
  • Catalog: Apoptosis
  • Density: 1.5±0.1 g/cm3
  • Boiling Point: 861.5±65.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 290.7±27.8 °C

VU 0364849

A potent, specific BMP receptor activin receptor-like kinase 3 (ALK3) antaognist with Ki of 5.4 nM; also inhibits ALK6 (Ki<1 nM) and ALK2 (Ki=42.77 nM), little to no affinity for ALK4/5, BMPR2, AMPK etc.; blocks SMAD phosphorylation in vitro and in vivo, and enhances liver regeneration after partial hepatectomy.

  • CAS Number: 1206711-14-9
  • MF: C27H25N5O2
  • MW: 451.520
  • Catalog: Apoptosis
  • Density: 1.3±0.1 g/cm3
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Glyphosate

Glyphosate is an herbicidal derivative of the amino acid glycine. Glyphosate targets and blocks a plant metabolic pathway not found in animals, the shikimate pathway, required for the synthesis of aromatic amino acids in plants[1].

  • CAS Number: 1071-83-6
  • MF: C3H8NO5P
  • MW: 169.073
  • Catalog: Apoptosis
  • Density: 1.7±0.1 g/cm3
  • Boiling Point: 465.8±55.0 °C at 760 mmHg
  • Melting Point: 230 °C (dec.)(lit.)
  • Flash Point: 235.5±31.5 °C

columbianadin

Columbianadin, a natural coumarin from, is known to have various biological activities including anti-inflammatory and anti-cancer effects.

  • CAS Number: 5058-13-9
  • MF: C19H20O5
  • MW: 328.359
  • Catalog: Apoptosis
  • Density: 1.2±0.1 g/cm3
  • Boiling Point: 482.3±45.0 °C at 760 mmHg
  • Melting Point: 166ºC
  • Flash Point: 212.7±28.8 °C

Indibulin

Indibulin (ZIO 301) , an orally applicable inhibitor of tubulin assembly, shows potent anticancer activity with a minimal neurotoxicity. Indibulin reduces inter-kinetochoric tension, produces aberrant spindles, activates mitotic checkpoint proteins Mad2 and BubR1, and induces mitotic arrest and apoptosis[1].

  • CAS Number: 204205-90-3
  • MF: C22H16ClN3O2
  • MW: 389.834
  • Catalog: Apoptosis
  • Density: 1.3±0.1 g/cm3
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Antitumor agent-70

Antitumor agent-70 (compound 8b) has anti-tumor activity and can induce cell apoptosis. Antitumor agent-70 inhibits multiple myeloma with an IC50 value of 0.12 μM. Antitumor agent-70 is a potential multi-targeted kinase inhibitor especially for c-Kit[1].

  • CAS Number: 2454133-88-9
  • MF: C21H18N4O2
  • MW: 358.39
  • Catalog: Apoptosis
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Ecteinascidin 770

Ecteinascidin 770 (ET-770) is a 1,2,3,4-tetrahydroisoquinoline alkaloid with potent anti-cancer activities; inhibits U373MG cells with an IC50 of 4.83 nM.

  • CAS Number: 114899-80-8
  • MF: C40H42N4O10S
  • MW: 770.84700
  • Catalog: Apoptosis
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

AZD4877

AZD4877 is another isostere to Ispinesib (HY-50759)and also a kinesin spindle protein (Eg5) inhibitor with IC50 of 2 nM.AZD4877 arrests cell mitosis, leads to the formation of the monopolar spindle phenotype and induces apoptosis. AZD4877 inhibits circulating peripheral blood mononuclear cells (PBMCs) and has anti-cancer activity[1][2][3][4][5].

  • CAS Number: 758722-49-5
  • MF: C28H33N5O2S
  • MW: 503.66
  • Catalog: Apoptosis
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

p53 Activator 7

p53 Activator 7 is a p53 mutation Y220C (MDM-2/p53) activator with an EC50 of 104 nM. p53 Activator 7 can bind to p53 mutant and restore its ability to bind DNA (WO2022213975A1; Example B-1)[1].

  • CAS Number: 2849340-59-4
  • MF: C27H32F3N4OP
  • MW: 516.54
  • Catalog: MDM-2/p53
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

NSC 66811

NSC 66811 is a MDM2-p53 inhibitor, with a Ki of 120 nM for binding to MDM2[1].

  • CAS Number: 6964-62-1
  • MF: C23H20N2O
  • MW: 340.418
  • Catalog: MDM-2/p53
  • Density: 1.2±0.1 g/cm3
  • Boiling Point: 529.5±45.0 °C at 760 mmHg
  • Melting Point: 145 °C
  • Flash Point: 274.0±28.7 °C

Tubulysin E

Tubulysin E is a highly cytotoxic peptide isolated from the myxobacterial species Archangium geophyra and Angiococcus disciformis. Tubulysin displays extremely potent cytotoxic activity in mammalian cells, including multidrug-resistant cell lines, with IC50 values in the lower nanomolar range[1]. Tubulysin E is a cytotoxic activity tubulysin which inhibits tubulin polymerization and leads to cell cycle arrest and apoptosis[2].

  • CAS Number: 309935-58-8
  • MF: C42H63N5O9S
  • MW: 814.04
  • Catalog: Apoptosis
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

SC-43

SC-43, a Sorafenib derivative, is a potent and orally active SHP-1 (PTPN6) agonist. SC-43 inhibits the phosphorylation of STAT3 and induces cell apoptosis. SC-43 has anti-fibrotic and anticancer effects[1][2].

  • CAS Number: 1400989-25-4
  • MF: C21H13ClF3N3O2
  • MW: 431.80
  • Catalog: Apoptosis
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A