A dye is a colored substance that has an affinity to the substrate to which it is being applied.

A new approach is presented for analysis of microplastics in environmental samples, based on selective fluorescent staining using Nile Red (NR), followed by density-based extraction and filtration. The dye adsorbs onto plastic surfaces and renders them fluorescent when irradiated with blue light. Fluorescence emission is detected using simple photography through an orange filter. Image-analysis allows fluorescent particles to be identified and counted. Magnified images can be recorded and tiled to cover the whole filter area, allowing particles down to a few micrometres to be detected. The solvatochromic nature of Nile Red also offers the possibility of plastic categorisation based on surface polarity characteristics of identified particles. This article details the development of this staining method and its initial cross-validation by comparison with infrared (IR) microscopy. Microplastics of different sizes could be detected and counted in marine sediment samples. The fluorescence staining identified the same particles as those found by scanning a filter area with IR-microscopy.

Cyanine dyes can be used to quantify the amount of dsDNA within a sample. The linearity of fluorescence, as function of DNA amount of six dyes, is obtained by measuring the fluorescence intensity at the optimal excitation and emission maxima.


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TMRM Perchlorate

TMRM Perchlorate is a cell-permeant cationic lipophilic red fluorescent dye (λex=530 nm, λem=592 nm).

  • CAS Number: 115532-50-8
  • MF: C25H25ClN2O7
  • MW: 500.928
  • Catalog: Dye Reagents
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: 274–276℃
  • Flash Point: N/A

6-FAM SE

6-FAM SE is another isomer of carboxyfluorescein. 6-FAM, SE is mainly used in sequencing of nucleic acids and labeling nucleotides.

  • CAS Number: 92557-81-8
  • MF: C25H15NO9
  • MW: 473.388
  • Catalog: Dye Reagents
  • Density: 1.7±0.1 g/cm3
  • Boiling Point: 788.1±70.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 430.4±35.7 °C

CY2-SE

CY2-SE is a dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides. Excitation (nm):492, Emission (nm): 510.

  • CAS Number: 186205-33-4
  • MF: C29H30IN3O6
  • MW: 643.469
  • Catalog: Dye Reagents
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Z-Gly-Gly-Arg-AMC acetate

Z-Gly-Gly-Arg-AMC acetate is a thrombin-specific fluorogenic substrate for testing of thrombin generation in PRP and platelet-poor plasma (PPP). Sequence: Z-Gly-Gly-Arg-AMC.

  • CAS Number: 2070009-61-7
  • MF: C30H37N7O9
  • MW: 639.66
  • Catalog: Peptides
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

D-Luciferin potassium salt

D-Luciferin potassium salt is the substrate of luciferases that catalyze the production of light in bioluminescent insects.

  • CAS Number: 115144-35-9
  • MF: C11H7KN2O3S2
  • MW: 318.41
  • Catalog: Dye Reagents
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Hoechst 33258 analog

Hoechst stains are part of a family of blue fluorescent dyes used to stain DNA.IC50 Value:Target: These Bis-benzimides were originally developed by Hoechst AG, which numbered all their compounds so that the dye Hoechst 33342 is the 33342nd compound made by the company. There are three related Hoechst stains: Hoechst 33258, Hoechst 33342, and Hoechst 34580. The dyes Hoechst 33258 and Hoechst 33342 are the ones most commonly used and they have similarexcitation/emission spectra. Both dyes are excited by ultraviolet light at around 350 nm, and both emit blue/cyan fluorescent light around anemission maximum at 461 nm. Unbound dye has its maximum fluorescence emission in the 510-540 nm range. Hoechst dyes are soluble in water and in organic solvents such as dimethyl formamide or dimethyl sulfoxide. Concentrations can be achieved of up to 10 mg/mL. Aqueous solutions are stable at 2-6 °C for at least six months when protected from light. For long-term storage the solutions are instead frozen at ≤-20 °C.The dyes bind to the minor groove of double-stranded DNA with a preference for sequences rich in adenine andthymine. Although the dyes can bind to all nucleic acids, AT-rich double-stranded DNA strands enhance fluorescence considerably.Hoechst dyes are cell-permeable and can bind to DNA in live or fixed cells. Therefore, these stains are often called supravital, which means that cells survive a treatment with these compounds. Cells that express specific ATP-binding cassette transporter proteins can also actively transport these stains out of their cytoplasm.

  • CAS Number: 258843-62-8
  • MF: C29H30N6O3
  • MW: 510.58700
  • Catalog: Dye Reagents
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

6-ROX

6-ROX is a fluorescent oligonucleotide marker, acts as an acceptor molecule coupled to 5-FAM as the donor in FRET imaging.Excitation:568nm. Emission:568nm

  • CAS Number: 194785-18-7
  • MF: C33H30N2O5
  • MW: 534.60200
  • Catalog: Dye Reagents
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Fast Green FCF

Fast Green FCF is a sea green triarylmethane food dye. Fast Green FCF is used as a quantitative stain for histones at alkaline pH after acid extraction of DNA. It is also used as a protein stain in electrophoresis. Its absorption maximum is at 625 nm.

  • CAS Number: 2353-45-9
  • MF: C37H34N2Na2O10S3
  • MW: 808.848
  • Catalog: Dye Reagents
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: 290ºC
  • Flash Point: N/A

Heptamethine cyanine dye-1

Heptamethine cyanine dye-1 is a near-infrared cyanine dye for fluorescence imaging in biological systems.

  • CAS Number: 162411-29-2
  • MF: C42H44ClIN2
  • MW: 739.170
  • Catalog: Dye Reagents
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

5-FAM SE

5-FAM SE is a single isomer, it is one of the most popular green fluorescent reagents used for labeling peptides, proteins and nucleotides. It has also been used to prepare various small fluorescent molecules.

  • CAS Number: 92557-80-7
  • MF: C25H15NO9
  • MW: 473.388
  • Catalog: Dye Reagents
  • Density: 1.7±0.1 g/cm3
  • Boiling Point: 778.7±70.0 °C at 760 mmHg
  • Melting Point: >300°C (dec.)
  • Flash Point: 424.7±35.7 °C

L-ANAP

L-ANAP is a genetically encodable and polarity-sensitive fluorescent unnatural amino acid (Uaa).

  • CAS Number: 1313516-26-5
  • MF: C15H16N2O3
  • MW: 272.29900
  • Catalog: Dye Reagents
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

HOE 33187

Hoechst stains are part of a family of blue fluorescent dyes used to stain DNA. HOE 33187 is a cell dye for DNA.IC50 Value:These Bis-benzimides were originally developed by Hoechst AG, which numbered all their compounds so that the dye Hoechst 33342 is the 33342nd compound made by the company. There are three related Hoechst stains: Hoechst 33258, Hoechst 33342, and Hoechst 34580. The dyes Hoechst 33258 and Hoechst 33342 are the ones most commonly used and they have similarexcitation/emission spectra. Both dyes are excited by ultraviolet light at around 350 nm, and both emit blue/cyan fluorescent light around anemission maximum at 461 nm. Unbound dye has its maximum fluorescence emission in the 510-540 nm range. Hoechst dyes are soluble in water and in organic solvents such as dimethyl formamide or dimethyl sulfoxide. Concentrations can be achieved of up to 10 mg/mL. Aqueous solutions are stable at 2-6 °C for at least six months when protected from light. For long-term storage the solutions are instead frozen at ≤-20 °C.The dyes bind to the minor groove of double-stranded DNA with a preference for sequences rich in adenine andthymine. Although the dyes can bind to all nucleic acids, AT-rich double-stranded DNA strands enhance fluorescence considerably.Hoechst dyes are cell-permeable and can bind to DNA in live or fixed cells. Therefore, these stains are often called supravital, which means that cells survive a treatment with these compounds. Cells that express specific ATP-binding cassette transporter proteins can also actively transport these stains out of their cytoplasm.in vitro: N/A in vivo: N/AClinical trial: N/A

  • CAS Number: 23623-08-7
  • MF: C25H24N6
  • MW: 408.498
  • Catalog: Dye Reagents
  • Density: 1.3±0.1 g/cm3
  • Boiling Point: 700.4±70.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 377.4±35.7 °C

Di-8-ANEPPS

Di-8-ANEPPS is a naphthylstyryl voltage-sensitive dye, shifting both their fluorescence excitation and emission spectra upon changes in Vm .

  • CAS Number: 157134-53-7
  • MF: C36H52N2O3S
  • MW: 592.87500
  • Catalog: Dye Reagents
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Rhodamine 123

Rhodamine 123 is a fluorescent dye (λex=503 nm, λem=527 nm).

  • CAS Number: 62669-70-9
  • MF: C21H17ClN2O3
  • MW: 380.824
  • Catalog: Dye Reagents
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: 235 °C
  • Flash Point: N/A

D-Lys(Z)-Pro-Arg-pNA diacetate

D-Lys(Z)-Pro-Arg-pNA (diacetate) diacetate is a chromogenic substrate. Sequence: D-Lys(Z)-Pro-Arg-pNA.

  • CAS Number: 108963-70-8
  • MF: C35H51N9O11
  • MW: 773.833
  • Catalog: Peptides
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

4-Methylumbelliferyl phosphate

4-methylumbelliferyl phosphate is a fluorogenic substrate of alkaline phosphatase.

  • CAS Number: 3368-04-5
  • MF: C10H9O6P
  • MW: 240.149
  • Catalog: Dye Reagents
  • Density: 1.6±0.1 g/cm3
  • Boiling Point: 513.6±60.0 °C at 760 mmHg
  • Melting Point: 215-218 °C
  • Flash Point: 264.4±32.9 °C

5(6)-TAMRA, SE [5-(and-6)-Carboxytetramethylrhodamine, succinimidyl ester]

5(6)-TAMRA SE is the amine-reactive, mixed isomer form of TAMRA, which is a dye for oligonucleotide labeling and automated DNA sequencing applications.

  • CAS Number: 246256-50-8
  • MF: C29H25N3O7
  • MW: 527.52500
  • Catalog: Dye Reagents
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Suc-Leu-Leu-Val-Tyr-AMC

Suc-Leu-Leu-Val-Tyr-AMC is a fluorogenic substrate. Sequence: Suc-Leu-Leu-Val-Tyr.

  • CAS Number: 94367-21-2
  • MF: C40H53N5O10
  • MW: 763.87600
  • Catalog: Peptides
  • Density: 1.249g/cm3
  • Boiling Point: 1116.8ºC at 760mmHg
  • Melting Point: N/A
  • Flash Point: 629.2ºC

Propidium Iodide

Propidium iodide is a red-fluorescent dye that can be used to stain cells.

  • CAS Number: 25535-16-4
  • MF: C27H34I2N4
  • MW: 668.395
  • Catalog: Dye Reagents
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: 220-225 °C (dec.)(lit.)
  • Flash Point: N/A

ATP-Red 1

ATP-Red 1 is a multisite-binding switchable fluorescent probe, and can selectively and rapidly responds to intracellular concentrations of ATP in living cells.

  • CAS Number: 1847485-97-5
  • MF: C34H36BN3O4
  • MW: 561.48
  • Catalog: Dye Reagents
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

TMRE

TMRE is a mitochondria specific dye (λex=550 nm, λem=575 nm).

  • CAS Number: 115532-52-0
  • MF: C26H27ClN2O7
  • MW: 514.955
  • Catalog: Dye Reagents
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

DiSBAC2(5)

DiBAC4(3) is a voltage-sensitive fluorescent dye (λex=490 nm, λem=505 nm).

  • CAS Number: 70363-83-6
  • MF: C27H40N4O6
  • MW: 516.63
  • Catalog: Dye Reagents
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

2-Aminoacridone

2-Aminoacridone is a widely used fluorophore (λexc=428 nm, λem=525 nm).

  • CAS Number: 27918-14-5
  • MF: C13H10N2O
  • MW: 210.231
  • Catalog: Dye Reagents
  • Density: 1.3±0.1 g/cm3
  • Boiling Point: 442.5±34.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 221.4±25.7 °C

6-Carboxyfluorescein Diacetate

6-CFDA is fluorescent polyanionic probe, and its derivatives such as 2′,7′-bis (2-carboxyethyl)-5(6)-carboxyfluorescein are used to measure changes in intracellular pH (pHi) and processes such as dendrimer aggregation and absorption.

  • CAS Number: 3348-03-6
  • MF: C25H16O9
  • MW: 460.389
  • Catalog: Dye Reagents
  • Density: 1.6±0.1 g/cm3
  • Boiling Point: 701.6±60.0 °C at 760 mmHg
  • Melting Point: 152–153°C
  • Flash Point: 245.1±26.4 °C

PL553

PL553 is a specific and high-affinity fluorigenic substrate of Leukotriene A4 hydrolase, with a λmax of 210 nm and λem of 410 nm.

  • CAS Number: 1456872-74-4
  • MF: C28H23F3N2O4
  • MW: 508.49
  • Catalog: Dye Reagents
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Ac-Leu-Arg-AMC

Ac-Leu-Arg-AMC is a fluorogenic peptide substrate.

  • CAS Number: 929621-79-4
  • MF: C24H34N6O5
  • MW: 486.56
  • Catalog: Peptides
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

AA-dUTP sodium salt

5-BrdUTP sodium salt is a TdT substrate which can be used to label the DNA double-strand breaks.

  • CAS Number: 102212-99-7
  • MF: C9H14BrN2Na4O14P3
  • MW: 639.00
  • Catalog: Dye Reagents
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

4-Chloro-7-nitrobenzofurazan

NBD-Cl is a nonfluorescent reagent which becomes highly fluorescent after reaction with thiol or amino groups.

  • CAS Number: 10199-89-0
  • MF: C6H2ClN3O3
  • MW: 199.551
  • Catalog: Dye Reagents
  • Density: 1.7±0.1 g/cm3
  • Boiling Point: 333.1±45.0 °C at 760 mmHg
  • Melting Point: 97-99 °C(lit.)
  • Flash Point: 155.3±28.7 °C

Sunset yellow

Sunset Yellow FCF is a petroleum-derived orange azo dye with a pH dependent maximum absorption at about 480 nm at pH 1 and 443 nm at pH 13. Sunset Yellow is used in food, cosmetics, and drugs.

  • CAS Number: 2783-94-0
  • MF: C16H10N2Na2O7S2
  • MW: 452.369
  • Catalog: Dye Reagents
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: 390°C (dec.)
  • Flash Point: N/A

CY7-SE

Cy7-SE dyes are widely used for labeling peptides, proteins and oligos etc. Cy7-SE dye conjugates are one type of the most common near infrared red fluorophores used in in vivo imaging applications. Excitation (nm): 749, Emission (nm): 767

  • CAS Number: 477908-53-5
  • MF: C39H45N3O10S2
  • MW: 779.91900
  • Catalog: Dye Reagents
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A