5-FAM SE structure
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Common Name | 5-FAM SE | ||
|---|---|---|---|---|
| CAS Number | 92557-80-7 | Molecular Weight | 473.388 | |
| Density | 1.7±0.1 g/cm3 | Boiling Point | 778.7±70.0 °C at 760 mmHg | |
| Molecular Formula | C25H15NO9 | Melting Point | >300°C (dec.) | |
| MSDS | Chinese USA | Flash Point | 424.7±35.7 °C | |
Use of 5-FAM SE5-FAM SE is a single isomer, it is one of the most popular green fluorescent reagents used for labeling peptides, proteins and nucleotides. It has also been used to prepare various small fluorescent molecules. |
| Name | 5-Carboxyfluorescein-N-hydroxysuccinimide Ester |
|---|---|
| Synonym | More Synonyms |
| Description | 5-FAM SE is a single isomer, it is one of the most popular green fluorescent reagents used for labeling peptides, proteins and nucleotides. It has also been used to prepare various small fluorescent molecules. |
|---|---|
| Related Catalog |
| Density | 1.7±0.1 g/cm3 |
|---|---|
| Boiling Point | 778.7±70.0 °C at 760 mmHg |
| Melting Point | >300°C (dec.) |
| Molecular Formula | C25H15NO9 |
| Molecular Weight | 473.388 |
| Flash Point | 424.7±35.7 °C |
| Exact Mass | 473.074677 |
| PSA | 139.67000 |
| LogP | 0.71 |
| Vapour Pressure | 0.0±2.8 mmHg at 25°C |
| Index of Refraction | 1.790 |
| Storage condition | −20°C |
| Personal Protective Equipment | Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter |
|---|---|
| Hazard Codes | Xi |
| RIDADR | NONH for all modes of transport |
| WGK Germany | 3 |
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A DNA-based molecular probe for optically reporting cellular traction forces.
Nature Methods 11(12) , 1229-32, (2014) We developed molecular tension probes (TPs) that report traction forces of adherent cells with high spatial resolution, can in principle be linked to virtually any surface, and obviate monitoring defo... |
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DNA sequencing with dye-labeled terminators and T7 DNA polymerase: effect of dyes and dNTPs on incorporation of dye-terminators and probability analysis of termination fragments.
Nucleic Acids Res. 20 , 2471, (1992) The incorporation of fluorescently labeled dideoxynucleotides by T7 DNA polymerase is optimized by the use of Mn2+, fluorescein analogs and four 2'-deoxyribonucleoside 5'-O-(1-thiotriphosphates) (dNTP... |
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Nonradioactive labeling of RNA.
Anal. Biochem. 233 , 124, (1996) A modified ribodinucleotide (named pSEEp) has been synthesized using commercially available components on a DNA synthesizer. The presence of a 3'-terminal primary amino group permits its coupling to a... |
| MFCD00077322 |
| 1-{[(3',6'-Dihydroxy-3-oxo-3H-spiro[2-benzofuran-1,9'-xanthen]-5-yl)carbonyl]oxy}-2,5-pyrrolidinedione |
| 5-Carboxyfluorescein N-succinimidyl ester |
| 2,5-Pyrrolidinedione, 1-[[(3',6'-dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-5-yl)carbonyl]oxy]- |
| 5-FAM SE |