5-FAM SE

5-FAM SE Structure
5-FAM SE structure
Common Name 5-FAM SE
CAS Number 92557-80-7 Molecular Weight 473.388
Density 1.7±0.1 g/cm3 Boiling Point 778.7±70.0 °C at 760 mmHg
Molecular Formula C25H15NO9 Melting Point >300°C (dec.)
MSDS Chinese USA Flash Point 424.7±35.7 °C

A DNA-based molecular probe for optically reporting cellular traction forces.

Nature Methods 11(12) , 1229-32, (2014)

We developed molecular tension probes (TPs) that report traction forces of adherent cells with high spatial resolution, can in principle be linked to virtually any surface, and obviate monitoring deformations of elastic substrates. TPs consist of DNA hairpins...

DNA sequencing with dye-labeled terminators and T7 DNA polymerase: effect of dyes and dNTPs on incorporation of dye-terminators and probability analysis of termination fragments.

Nucleic Acids Res. 20 , 2471, (1992)

The incorporation of fluorescently labeled dideoxynucleotides by T7 DNA polymerase is optimized by the use of Mn2+, fluorescein analogs and four 2'-deoxyribonucleoside 5'-O-(1-thiotriphosphates) (dNTP alpha S's). The one-tube extension protocol was tested on ...

Nonradioactive labeling of RNA.

Anal. Biochem. 233 , 124, (1996)

A modified ribodinucleotide (named pSEEp) has been synthesized using commercially available components on a DNA synthesizer. The presence of a 3'-terminal primary amino group permits its coupling to a range of nonradioactive labels, exemplified here by fluore...

Lau, S.K., et al.

J. Chromatogr. A. 809 , 203, (1998)