![]() 5-羧基荧光素琥珀酰亚胺酯结构式
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常用名 | 5-羧基荧光素琥珀酰亚胺酯 | 英文名 | 5-FAM SE |
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CAS号 | 92557-80-7 | 分子量 | 473.388 | |
密度 | 1.7±0.1 g/cm3 | 沸点 | 778.7±70.0 °C at 760 mmHg | |
分子式 | C25H15NO9 | 熔点 | >300°C (dec.) | |
MSDS | 中文版 美版 | 闪点 | 424.7±35.7 °C |
A DNA-based molecular probe for optically reporting cellular traction forces.
Nature Methods 11(12) , 1229-32, (2014) We developed molecular tension probes (TPs) that report traction forces of adherent cells with high spatial resolution, can in principle be linked to virtually any surface, and obviate monitoring deformations of elastic substrates. TPs consist of DNA hairpins... |
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DNA sequencing with dye-labeled terminators and T7 DNA polymerase: effect of dyes and dNTPs on incorporation of dye-terminators and probability analysis of termination fragments.
Nucleic Acids Res. 20 , 2471, (1992) The incorporation of fluorescently labeled dideoxynucleotides by T7 DNA polymerase is optimized by the use of Mn2+, fluorescein analogs and four 2'-deoxyribonucleoside 5'-O-(1-thiotriphosphates) (dNTP alpha S's). The one-tube extension protocol was tested on ... |
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Nonradioactive labeling of RNA.
Anal. Biochem. 233 , 124, (1996) A modified ribodinucleotide (named pSEEp) has been synthesized using commercially available components on a DNA synthesizer. The presence of a 3'-terminal primary amino group permits its coupling to a range of nonradioactive labels, exemplified here by fluore... |
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Lau, S.K., et al.
J. Chromatogr. A. 809 , 203, (1998)
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