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25535-16-4

25535-16-4 structure
25535-16-4 structure
  • Name: Propidium Iodide
  • Chemical Name: propidium iodide
  • CAS Number: 25535-16-4
  • Molecular Formula: C27H34I2N4
  • Molecular Weight: 668.395
  • Catalog: Dye Reagents
  • Create Date: 2018-03-19 08:00:00
  • Modify Date: 2024-01-02 14:17:31
  • Propidium iodide is a red-fluorescent dye that can be used to stain cells.
Name propidium iodide
Synonyms 3,8-Diamino-5-[3-(diaethyl-methyl-ammonio)-propyl]-6-phenyl-phenanthridinium,Dijodid
propidium iodine
3,8-diamino-5-[3-(diethylmethylammonio)propyl]-6-phenylphenanthridinium diiodide
3,8-Diamino-5-{3-[diethyl(methyl)ammonio]propyl}-6-phenylphenanthridinium diiodide
3,8-Diamino-5-(3-Diethylaminopropyl)-6-Phenylphenanthridinium Iodide methIodide
(3,8-diamino-5-(3-diethylmethylamino)propyl)-6-phenylphenanthridinium diiodide
Propidium Iodide
3-(3,8-diamino-6-phenylphenanthridin-5-ium-5-yl)propyl-diethyl-methylazanium,diiodide
EINECS 247-081-0
Propidium iodide solution
Phenanthridinium, 3,8-diamino-5-[3-(diethylmethylammonio)propyl]-6-phenyl- , diiodide
Phenanthridinium, 3,8-diamino-5-[3-(diethylmethylammonio)propyl]-6-phenyl-, iodide (1:2)
MFCD00011921
3,8-diamino-5-[3-(diethyl-methyl-ammonio)-propyl]-6-phenyl-phenanthridinium,diiodide
3,8-Diamino-5-(3-(diethyl(methyl)ammonio)propyl)-6-phenylphenanthridin-5-ium iodide
Propidium diiodide
Propidium (Iodide)
Description Propidium iodide is a red-fluorescent dye that can be used to stain cells.
Related Catalog
In Vitro Propidium iodide is a cell-membrane impermeable dye with characteristic excitation maximum at 535 nm and emission maximum at 617 nm which intercalates with nucleic acids with a stoichiometry of one dye per 4-5 base pairs with little sequence preference. Propidium iodide has evidenced of having no toxic effects on neurons, being today’s most common marker for membrane integrity and cell viability when applied prior to fixation (pre-fixation propidium iodide staining method). The pre-fixation staining has been widely used for quantitative assessments of neuronal cell decline in models of acute neurodegeneration, visualized as intensely labeled PI+-pycnotic nuclei of degenerating neurons [1]. Propidium iodide cannot cross the membrane of live cells, making it useful to measure the percentage of apoptotic cells by flow-cytometric analysis. The flow cytometric data shows an excellent correlation with the results obtained with both electrophoretic and colorimetric methods. This new rapid, simple and reproducible method proves useful for assessing apoptosis of specific cell populations in heterogeneous tissues such as bone marrow, thymus and lymph nodes[2].
Cell Assay Flow cytometric analysis: Propidium iodide is prepared in in 0.1% sodium citrate plus 0.1% Triton X-100 (50 μg/mL). The 200 ×g centrifuged cell pellet is gently resuspended in 1.5 mL hypotonlc fluorochrome solution (Propidium iodide 50 μg/mL), in 12×75 polypropylene tubes. The tubes are placed at 4°C in the dark overnight before the flow cytometric analysis. The propidium Iodide fluorescence of individual nuclei is measured using a FACScan flow cytometer. The nuclei traverses the light beam of a 488 nm Argon laser. A 560 nm dichrolc nurror (DM 570) and a 600 nm band pass filter (bandwidth 35 nm) are used for collecting the red fluorescence due to propidium Iodide staining of DNA and the data are registered on a logarithmic scale[2].
References

[1]. Hezel M, et al. Propidium iodide staining: a new application in fluorescence microscopy for analysis of cytoarchitecture in adult and developing rodent brain. Micron. 2012 Oct;43(10):1031-8.

[2]. A rapid and simple method for measuring thymocyte apoptosis by propidium iodidestaining and flow cytometry. J Immunol Methods. 1991 Jun 3;139(2):271-9.
Melting Point 220-225 °C (dec.)(lit.)
Molecular Formula C27H34I2N4
Molecular Weight 668.395
Exact Mass 668.087280
PSA 55.92000
LogP 0.15880
Storage condition 2-8°C

CHEMICAL IDENTIFICATION

RTECS NUMBER :
SF7949600
CHEMICAL NAME :
Phenanthridinium, 3,8-diamino-5-(3-(diethylmethylammonio)propyl)-6-phen yl-, diiodide
CAS REGISTRY NUMBER :
25535-16-4
LAST UPDATED :
199410
DATA ITEMS CITED :
5
MOLECULAR FORMULA :
C27-H34-N4.2I
MOLECULAR WEIGHT :
668.45

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

TYPE OF TEST :
LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE :
Subcutaneous
SPECIES OBSERVED :
Rodent - mouse
DOSE/DURATION :
16 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value

MUTATION DATA

TYPE OF TEST :
Gene conversion and mitotic recombination
TEST SYSTEM :
Yeast - Saccharomyces cerevisiae
DOSE/DURATION :
50 umol/L/3D (Continuous)
REFERENCE :
BBRCA9 Biochemical and Biophysical Research Communications. (Academic Press, Inc., 1 E. First St., Duluth, MN 55802) V.1- 1959- Volume(issue)/page/year: 84,501,1978
Symbol GHS07 GHS08
GHS07, GHS08
Signal Word Warning
Hazard Statements H315-H319-H335-H341
Precautionary Statements P261-P281-P305 + P351 + P338
Personal Protective Equipment Eyeshields;Gloves
Hazard Codes Xi
Risk Phrases 36/37/38
Safety Phrases S26
RIDADR NONH for all modes of transport
WGK Germany 3
RTECS SF7949600
Precursor  0

DownStream  1


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