Dayang Chem (Hangzhou) Co., Ltd.
China
Product Name: Tetramethylrhodamine ethyl ester perchlorate (TMRE)
Price: $Inquiry/100g $Inquiry/1kg $Inquiry/100kg $Inquiry/1000kg
Purity: 98.0%
Stocking Period: Inquiry
Contact: Ms Wang

115532-52-0

115532-52-0 structure

115532-52-0 structure

Name: TMRE
Chemical Name: [6-(dimethylamino)-9-(2-ethoxycarbonylphenyl)xanthen-3-ylidene]-dimethylazanium,perchlorate
CAS Number: 115532-52-0
Molecular Formula: C₂₆H₂₇ClN₂O₇
Molecular Weight: 514.955
Catalog: Dye Reagents
Create Date: 2018-04-13 08:00:00
Modify Date: 2024-01-08 16:32:52
TMRE is a mitochondria specific dye (λex=550 nm, λem=575 nm).

Name	[6-(dimethylamino)-9-(2-ethoxycarbonylphenyl)xanthen-3-ylidene]-dimethylazanium,perchlorate
Synonyms	Tetramethylrhodamine ethyl ester perchlorate TMRE [Tetramethylrhodamine,ethyl ester,perchlorate] 3,6-Bis(dimethylamino)-9-(2-(ethoxycarbonyl)phenyl)xanthylium perchlorate MFCD00467973 TMRE 6-(Dimethylamino)-9-[2-(ethoxycarbonyl)phenyl]-N,N-dimethyl-3H-xanthen-3-iminium perchlorate RB3096

Description	TMRE is a mitochondria specific dye (λex=550 nm, λem=575 nm).
Related Catalog	Signaling Pathways >> Others >> Others Dye Reagents Research Areas >> Others
In Vitro	TMRE is a mitochondria specific dye (λex=550 nm, λem=575 nm)[1]. Multidirectional dynamic movement of TMRE is observed in epithelial cells and bidirectional dynamic movement is seen in the superficial cortical fiber cells of live bovine lenses. In the epithelium, the movement of TMRE fluorescence is up to 5 μm/min whereas in the superficial cortex the observed movement is up to 18.5 μm/min. The movement of TMRE fluorescence is abolished with treatment of the uncoupler, carbonyl cyanide m-chlorophenylhydrazone (CCCP)[2].
Cell Assay	The entire experiment should be performed at room temperature because temperature will directly impact mitochondrial transmembrane potential and TMRE staining. Cells should never be placed, centrifuged, incubated, or washed at 4°C or have ice-cold buffers or media added. Treat the cells with a cytotoxic stimulus. Harvest cells and resuspend at 5×105 cells/mL in culture medium containing 150 nM TMRE. Incubate for 5 min at room temperature in the dark. Add Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP) (5 μM final concentration) to an aliquot of untreated cells and incubate for 5 min at room temperature in the dark. Turn on the appropriate laser on the flow cytometer. Set up a histogram plot to detect TMRE using log scale[1].
References	[1]. Crowley LC, et al. Measuring Mitochondrial Transmembrane Potential by TMRE Staining. Cold Spring Harb Protoc. 2016 Dec 1;2016(12):pdb.prot087361. [2]. Bantseev V, et al. Confocal laser scanning microscopy imaging of dynamic TMRE movement in the mitochondria of epithelial and superficial cortical fiber cells of bovine lenses. Mol Vis. 2005 Jul 14;11:518-23.

Molecular Formula	C₂₆H₂₇ClN₂O₇
Molecular Weight	514.955
Exact Mass	514.150696
PSA	120.19000
LogP	6.05710
Storage condition	2-8℃

Symbol	GHS07
Signal Word	Warning
Hazard Statements	H302-H315-H319-H332
Precautionary Statements	P305 + P351 + P338
Personal Protective Equipment	dust mask type N95 (US);Eyeshields;Gloves
Hazard Codes	Xn: Harmful;
Risk Phrases	R20/22
Safety Phrases	26-36
RIDADR	NONH for all modes of transport