Name | succinyl-Leu-Leu-Val-Tyr-7-amino-4-methylcoumarin |
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Synonyms |
z-llvy-amc
suc-leu-leu-val-tyr-amc suc-llvy-amc suc-leu-leu-val-tyr-7-amino-4-methylcoumarin chymotrypsin substrate iii,fluorogenic n-succinyl-leu-leu-val-tyr 7-amido-4-methylcoumarin MFCD00080248 suc-leu-leu-val-tyr-mca |
Description | Suc-Leu-Leu-Val-Tyr-AMC is a fluorogenic substrate. Sequence: Suc-Leu-Leu-Val-Tyr. |
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Related Catalog | |
In Vitro | Suc-Leu-Leu-Val-Tyr-AMC (Suc-LLVY) is a membrane-permeable calpain-specific fluorogenic substrate, pteolytic hydrolysis of the peptidyl-7-amino bond liberates the highly fluorescent 7-amino-4-methylcoumarin (AMC) moiety[1]. The effectof TGF-β on hydrolysis of these substrates (e.g Suc-Leu-Leu-Val-Tyr-AMC) are assessed. Biliary epithelial H69 cells are incubated with 10, 1, 0.1, or 0 ng/mL TGF-β for 24 h. Substrate hydrolysis is then fluorometrically assessed in cytosolic extracts. Basal activity is 1.12, 8.33, and 14.52 nmol AMC/mg protein/min for suc-LLVY-AMC, z-LLE-AMC, and z-LLL-AMC hydrolysis, respectively[2]. |
Kinase Assay | Immunoprecipitation is carried out for the two sets of samples, using the same amount of protein. The 20 S and 26 S proteasome immunoprecipitates are washed with 50 mM Hepes/KOH (pH 7.5), and 50 mM Hepes/KOH (pH 7.5) containing 2 mMATP, respectively, prior to the determination of peptidase activity using 50 μM suc-Leu- Leu-Val-Tyr-AMC as substrate in these buffers[3]. |
References |
Density | 1.249g/cm3 |
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Boiling Point | 1116.8ºC at 760mmHg |
Molecular Formula | C40H53N5O10 |
Molecular Weight | 763.87600 |
Flash Point | 629.2ºC |
Exact Mass | 763.37900 |
PSA | 233.24000 |
LogP | 5.17700 |
Index of Refraction | 1.577 |
Storage condition | −20°C |
Personal Protective Equipment | Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter |
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RIDADR | NONH for all modes of transport |
WGK Germany | 3 |