Stem cells are required for continuous tissue maintenance within diverse organs, stem cell activity is often externally dictated by the microenvironment (the niche) so that stem cell output is precisely shaped to meet homeostatic needs or regenerative demands. Several key signaling pathways have been shown to play essential roles in this regulatory capacity. Specifically, the JAK/STAT, Hedgehog, Wnt, Notch, Smad, PI3K/phosphatase and tensin homolog, and NK-κB signaling pathways have all been shown experimentally to mediate various stem cell properties, such as self-renewal, cell fate decisions, survival, proliferation, and differentiation.

Recent studies mainly focus on cancer stem cell, induced pluripotent stem cell, neural stem cell and maintenance of embryonic stem cell pluripotency. Cancer stem cells (CSCs) have been believed to be responsible for tumor initiation, growth, and recurrence. Numerous agents have been developed to specifically target CSCs by suppressing the expression of pluripotency maintaining factors Nanog, Oct-4, Sox-2, and c-Myc and transcription of GLI. Induced pluripotent stem cells (iPSCs) have the capacity to differentiate into various types of cells, and a self-renewing resource, and scientists can experiment with an unlimited number of pluripotent cells to perfect the process of targeted differentiation, transplantation, and more, for personalized medicine. Novel pathological mechanisms have been elucidated, new drugs originating from iPSC screens are in the pipeline and the first clinical trial using human iPSC-derived products has been initiated.

References:
[1] Clevers H, et al. Science. 2014 Oct 3;346(6205):1248012.
[2] Matsui WH. Medicine (Baltimore). 2016 Sep;95(1 Suppl 1):S8-S19.
[3] Koury J, et al. Stem Cells Int. 2017;2017:2925869.
[4] Garg A, et al. Cells. 2017 Feb 2;6(1). doi: 10.3390/cells6010004.


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4-[(1R)-1-Aminoethyl]-N-1H-pyrrolo[2,3-b]pyridin-4-ylbenzamide hydrochloride

Y-33075 hydrochloride (Y-39983) is a selective ROCK inhibitor derived from Y-27632, and is more potent than Y-27632, with an IC50 of 3.6 nM.

  • CAS Number: 471843-75-1
  • MF: C16H17ClN4O
  • MW: 316.79
  • Catalog: ROCK
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

O4I1

O4I1 is as a potent Oct3/4 inducer.Target: Oct3/4O4I1 enhances Oct3/4 gene expression, increases Oct3/4 protein levels, and promotes Oct3/4 mediated transcriptional activation. O4I1 activates Oct3/4 at the transcriptional and translational levels in diverse human cell lines. O4I1 promotes Oct3/4 expression in untransfected HEK293 cells quantified by qRT-PCR after 72 h of treatment. O4I1 is sufficient to enhance Oct3/4 overexpression 2-fold and 3-fold after 48 and 72 h of treatment, respectively.

  • CAS Number: 175135-47-4
  • MF: C16H15NO2
  • MW: 253.296
  • Catalog: Oct3/4
  • Density: 1.1±0.1 g/cm3
  • Boiling Point: 424.5±30.0 °C at 760 mmHg
  • Melting Point: 140ºC
  • Flash Point: 154.8±17.8 °C

MRT-83 hydrochloride

MRT-83 (hydrochloride) is the potent antagonist of Smoothened (Smo) receptor. MRT-83 (hydrochloride) inhibits the Hedgehog (Hh) signaling pathway and BODIPY-cyclopamine binding to human Smo. MRT-83 (hydrochloride) has the potential for researching cancer disease[1].

  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

YO-01027

YO-01027 (Dibenzazepine;DBZ) is a potent γ-secretase inhibitor with IC50 values of 2.92±0.22 and 2.64±0.30 nM for Notch and APPL cleavage, respectively.

  • CAS Number: 209984-56-5
  • MF: C26H23F2N3O3
  • MW: 463.476
  • Catalog: γ-secretase
  • Density: 1.4±0.1 g/cm3
  • Boiling Point: 801.3±65.0 °C at 760 mmHg
  • Melting Point: 257-259ºC
  • Flash Point: 438.4±34.3 °C

JAK-IN-14

JAK-IN-14 is a potent and selective JAK1 inhibitor, with an IC50 of <5 μM. JAK-IN-14 is >8-fold more selective for JAK1 than JAK2 and JAK3 (Patent WO2016119700A1, compound 16)[1].

  • CAS Number: 1973485-06-1
  • MF: C19H15FN4O
  • MW: 334.35
  • Catalog: JAK
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Casein kinase 1δ-IN-8

Casein kinase 1δ-IN-8 (compound 494) is an inhibitor of Casein kinase 1δ, Casein kinase 1δ-IN-8 can be used in the treatment of neurodegenerative disorders such as Alzheimer's disease[1].

  • CAS Number: 851396-73-1
  • MF: C19H14FN5OS
  • MW: 379.41
  • Catalog: Casein Kinase
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

STAT6-IN-3

STAT6-IN-3 (Compound 18a) is a STAT6 inhibitor (IC50= 44 nM). STAT6-IN-3 targets the Src Homology 2 (SH2) domain of STAT6. STAT6-IN-3 can be used for research of inflammation such as asthma[1].

  • CAS Number: 371919-80-1
  • MF: C32H35IN3O7P
  • MW: 731.51
  • Catalog: STAT
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Asiaticoside

Asiaticoside, a trisaccaride triterpene from Centella asiatica, suppresses TGF-β/Smad signaling through inducing Smad7 and inhibiting TGF-βRI and TGF-βRII in keloid fibroblasts; Asiaticoside shows antioxidant, anti-inflammatory, and anti-ulcer properties.

  • CAS Number: 16830-15-2
  • MF: C48H78O19
  • MW: 959.12
  • Catalog: TGF-beta/Smad
  • Density: 1.4±0.1 g/cm3
  • Boiling Point: 949.4±65.0 °C at 760 mmHg
  • Melting Point: 235-238ºC
  • Flash Point: 268.4±27.8 °C

Hu7691

Hu7691 is an orally active, selective Akt inhibitor with IC50s of 4.0 nM, 97.5 nM, 28 nM for Akt1, Akt2 and Akt3, respectively. Hu7691 inhibits tumor growth and enables decrease of cutaneous toxicity in mice[1].

  • CAS Number: 2360523-76-6
  • MF: C22H22ClF3N4O
  • MW: 450.88
  • Catalog: ROCK
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

JAK3/BTK-IN-5

JAK3/BTK-IN-5 is a potent inhibitor of JAK3/BTK. BTK and JAK3 are two important targets for autoimmune diseases. Simultaneous inhibition of the BTK/JAK3 signalling pathway exhibits synergistic effects. JAK3/BTK-IN-5 has the potential for the research of JAK3 kinase and/or BTK-related diseases (extracted from patent WO2021147953A1, compound 35)[1]

  • CAS Number: 2673196-75-1
  • MF: C19H22ClN7O2
  • MW: 415.88
  • Catalog: JAK
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Triptonide

Triptonide(NSC 165677; PG 492), extracted from Tripterygium wilfordii Hook, inhibited the proliferation of mouse splenocytes induced by suboptimal concentration of concanavalin A or lipopolysaccharide at concentrations of 0.02, 0.1, and 0.5 mg/ml.

  • CAS Number: 38647-11-9
  • MF: C20H22O6
  • MW: 358.385
  • Catalog: Wnt
  • Density: 1.5±0.1 g/cm3
  • Boiling Point: 581.1±50.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 257.8±30.2 °C

Z-Ile-Leu-aldehyde

Z-Ile-Leu-aldehyde(Z-IL-CHO; GSI-XII) is a potent gamma-Secretase inhibitor; Notch signaling inhibitor.IC50 value:Target: gamma-Secretase inhibitorin vitro: GSI-XII induces apoptosis of murine MOPC315.BM myeloma cells with high Notch activity. GSI XII impairs murine osteoclast differentiation of receptor activator of NF-κB ligand (RANKL)-stimulated RAW264.7 cells in vitro [1]. Notch-signaling inhibition in HRS cells by the γ-secretase inhibitor (GSI) XII results in decreased alternative p52/RelB NF-κB signaling, interfering with processing of the NF-κB2 gene product p100 into its active form p52 [2]. GSI treatment induced morphologic erythroid differentiation and promoted hemoglobin production. GSI treatment suppressed short-term growth and colony formation, while treatment with GSI-XXI promoted the growth of AA cells [3].in vivo: In the murine MOPC315.BM myeloma model GSI XII has potent anti-MM activity and reduces osteolytic lesions as evidenced by diminished myeloma-specific monoclonal immunoglobulin (Ig)-A serum levels and quantitative assessment of bone structure changes via high-resolution microcomputed tomography scans [1].

  • CAS Number: 161710-10-7
  • MF: C20H30N2O4
  • MW: 362.46300
  • Catalog: γ-secretase
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Silmitasertib (CX-4945)

CX-4945 is a potent, selective and oral casein kinase 2 (CK2) inhibitor with a Ki of 0.38 nM.

  • CAS Number: 1009820-21-6
  • MF: C19H12ClN3O2
  • MW: 349.771
  • Catalog: Autophagy
  • Density: 1.5±0.1 g/cm3
  • Boiling Point: 568.5±50.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 297.6±30.1 °C

Tyk2-IN-7

Tyk2-IN-7 (Compound 48) is a TYK2 JH2 inhibitor, binds to TYK2 JH2 domain with IC50 and Ki.app of 0.00053 μM and 0.00007 μM, respectively. Tyk2-IN-7 (Compound 48) provides a highly selective alternative to conventional TYK2 orthosteric inhibitors, inhibits TYK2/JAK1/JAK2 kinase domain. Tyk2-IN-7 (Compound 48) provides robust inhibition in a mouse IL-12-induced IFNγ pharmacodynamic model as well as efficacy in an IL-23 and IL-12-dependent mouse colitis model[1].

  • CAS Number: 1609391-90-3
  • MF: C18H15D3N6O3S
  • MW: 401.46
  • Catalog: JAK
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Garcinone D

Garcinone D, a natural xanthone from mangosteen, promotes the proliferation of C17.2 neural stem cell. Garcinone D increases the protein levels of phosphorylated signal transducer and activator of transcription 3 (p-STAT3), Cyclin D1 and nuclear factor erythroid 2-related factor (Nrf2), heme oxygenase-1 (HO-1) in concentration- and time- dependent manners[1].

  • CAS Number: 107390-08-9
  • MF: C24H28O7
  • MW: 428.475
  • Catalog: STAT
  • Density: 1.304
  • Boiling Point: 674.2±55.0 °C at 760 mmHg
  • Melting Point: 202-204 ºC
  • Flash Point: 231.2±25.0 °C

resveratrol triacetate

Triacetylresveratrol, an acetylated analog of Resveratrol. Triacetylresveratrol decreases the phosphorylation of STAT3 and NF-κB in a dose- and time- dependent manner in PANC-1 and BxPC-3 cells. Anticancer effects[1].

  • CAS Number: 42206-94-0
  • MF: C20H18O6
  • MW: 354.353
  • Catalog: STAT
  • Density: 1.2±0.1 g/cm3
  • Boiling Point: 504.8±50.0 °C at 760 mmHg
  • Melting Point: 117.0 to 121.0 °C
  • Flash Point: 221.4±30.2 °C

JAK-IN-15

JAK-IN-15 is a JAK inhibitor. WO2016119700A1 (Compound 15)[1].

  • CAS Number: 1973485-05-0
  • MF: C22H23FN4O3S
  • MW: 442.51
  • Catalog: JAK
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

PF-3084014

Nirogacestat (PF-3084014) is a reversible, noncompetitive, and selective γ-secretase inhibitor with IC50 of 6.2 nM.

  • CAS Number: 1290543-63-3
  • MF: C27H41F2N5O
  • MW: 489.64400
  • Catalog: γ-secretase
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Baricitinib

Baricitinib is a selective and orally bioavailable JAK1 and JAK2 inhibitor with IC50s of 5.9 nM and 5.7 nM, respectively.

  • CAS Number: 1187594-09-7
  • MF: C16H17N7O2S
  • MW: 371.417
  • Catalog: JAK
  • Density: 1.6±0.1 g/cm3
  • Boiling Point: 707.2±70.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 381.5±35.7 °C

RLX-33

RLX-33 is a potent, selective and blood-brain barrier (BBB) penetrant relaxin family peptide 3 (RXFP3) antagonist, also blocks relaxin-3-induced ERK1/2 phosphorylation, with IC50 values of 2.36 μM for RXFP3, 7.82 and 13.86 μM for ERK1 and ERK2 phosphorylation, respectively. RLX-33 can block the stimulation of food intake induced by the RXFP3-selective agonist R3/I5 in rats. RLX-33 can be used for the research of metabolic syndrome[1].

  • CAS Number: 2784577-71-3
  • MF: C24H19ClN4O4
  • MW: 462.89
  • Catalog: ERK
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

LX7101

LX7101 is a potent inhibitor of LIMK and ROCK2 with IC50 values of 24, 1.6 and 10 nM for LIMK1, LIMK2 and ROCK2, respectively; also inhibits PKA with an IC50 less than 1 nM.

  • CAS Number: 1192189-69-7
  • MF: C23H29N7O3
  • MW: 451.52100
  • Catalog: ROCK
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

GANT58

GANT 58 is a potent Gli antagonist that inhibits GLI1-induced transcription with IC50 of 5 μM.

  • CAS Number: 64048-12-0
  • MF: C24H16N4S
  • MW: 392.476
  • Catalog: Gli
  • Density: 1.3±0.1 g/cm3
  • Boiling Point: 414.1±40.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 169.0±17.7 °C

JAK2-IN-4

JAK2-IN-4 (compound 16h) is a selective JAK2/JAK3 inhibitor, with IC50 values of 0.7 nM and 23.2 nM for JAK2 and JAK3, respectively[1].

  • CAS Number: 1438284-00-4
  • MF: C23H27N5O4S
  • MW: 469.56
  • Catalog: JAK
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

LY-411575 (isomer 1)

LY-411575 isomer 1 is an isomer of LY411575, which is a potent γ-secretase inhibitor.

  • CAS Number: 209984-58-7
  • MF: C26H23F2N3O4
  • MW: 479.47500
  • Catalog: γ-secretase
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Enniatin B1

Enniatin B1 is a Fusarium mycotoxin. Enniatin B1 inhibits acyl-CoA: cholesterol acyltransferase (ACAT) activity with an IC50 of 73 μM in an enzyme assay using rat liver microsomes[1]. Enniatin B1 crosss the blood-brain barrier[2]. Enniatin B1 decreases the activation of ERK (p44/p42). Enniatin B1 inhibits moderately TNF-α-induced NF-κB activation[3].

  • CAS Number: 19914-20-6
  • MF: C34H59N3O9
  • MW: 653.847
  • Catalog: ERK
  • Density: 1.0±0.1 g/cm3
  • Boiling Point: 833.8±65.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 458.0±34.3 °C

AT-9283 L-lactate

AT9283 lactic acid is a multi-targeted kinase inhibitor with potent activity against Aurora A/B, JAK2/3, Abl (T315I) and Flt3 (IC50s ranging from 1 to 30 nM). AT9283 lactic acid inhibits growth and survival of multiple solid tumors in vitro and in vivo[1][2].

  • CAS Number: 896466-76-5
  • MF: C22H29N7O5
  • MW: 471.51
  • Catalog: Apoptosis
  • Density: 1.5±0.1 g/cm3
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

VX-11e

VX-11e is a potent, selective, and orally bioavailable inhibitor of ERK with Ki < 2 nM.

  • CAS Number: 896720-20-0
  • MF: C24H20Cl2FN5O2
  • MW: 500.352
  • Catalog: ERK
  • Density: 1.4±0.1 g/cm3
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

H-Pro-Ser-OH

Prolylserine, a dipeptide, is an inhibitor of melanogenesis production in Mel-Ab cells. Prolylserine decreases expression of microphthalmia-associated transcription factor (MITF) and tyrosinase, induces phorphosylation of ERK, but not cAMP response element binding protein (CREB)[1].

  • CAS Number: 71835-80-8
  • MF: C8H14N2O4
  • MW: 202.21
  • Catalog: ERK
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

3F8

3F8 is a potent and selective GSK-3β inhibitor that could be useful as new reagent and potential therapeutic candidate for GSK3 related diseases[1].

  • CAS Number: 159109-11-2
  • MF: C15H14N2O4
  • MW: 286.28300
  • Catalog: GSK-3
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

TMCB

TMCB is a selective, ATP-competitive CK2 (casein kinase II) inhibitor with distinct Ki values of 83 nM and 21 nM for the two different catalytic CK2 subunits α and α', respectively[1].

  • CAS Number: 905105-89-7
  • MF: C11H9Br4N3O2
  • MW: 534.82400
  • Catalog: Casein Kinase
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A