The PI3K/Akt/mTOR signaling pathways is crucial to many aspects of cell growth and survival, in physiological as well as in pathological conditions. PI3Ks constitute a lipid kinase family. Class I PI3Ks are heterodimers composed of a catalytic (CAT) subunit (i.e., p110) and an adaptor/regulatory subunit (i.e., p85), and can be further divided into two subclasses: subclass IA (PI3Kα, β, and δ), which is activated by receptors with protein tyrosine kinase activity, and subclass IB (PI3Kγ), which is activated by receptors coupled with G proteins. Akt kinases belong to the AGC kinase family, related to AMP/GMP kinases and protein kinase C. mTOR is a key protein evolutionarily conserved from yeast to man and is essential for life. The mTORC1 complex is made up of mTOR, Raptor, mLST8, and PRAS40, and the mTORC2 complex is composed of mTOR, Rictor, Sin1, and mLST8.

Upon ligand binding, phosphorylated tyrosine residing in activated RTKs will bind to p85, then release the catalytic subunit p110. Activated p110 phosphorylated the PIP2 into the second messenger PIP3, and this reaction can be reversed by the PI3K antagonist PTEN. PIP3 will recruit the downstream Akt to inner membranes and phosphorylates Akt on its serine/threonine kinase sites (Thr308 and Ser473). Activated Akt is involved in the downstream mTORC1 mediated response to biogenesis of protein and ribosome.

Many genes belonging to the PI3K/Akt pathway have been implicated in the pathophysiology of solid tumors and sensitivity/resistance to chemotherapy. More and more studies are now focusing on the translational relevance of targeting these pathways in cancer therapy.

References:
[1] Porta C, et al. Front Oncol. 2014 Apr 14;4:64.
[2] Follo MY, et al. Adv Biol Regul. 2015 Jan;57:10-6.
[3] Li X, et al. Oncotarget. 2016 May 31;7(22):33440-50.


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ETP-46464

ETP-46464 is an effective mTOR and ATR inhibitor with IC50s of 0.6 and 14 nM, respectively.

  • CAS Number: 1345675-02-6
  • MF: C30H22N4O2
  • MW: 470.521
  • Catalog: ATM/ATR
  • Density: 1.3±0.1 g/cm3
  • Boiling Point: 709.3±60.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 382.7±32.9 °C

CRTh2 antagonist 3

CRTh2 antagonist 3 is a potent chemoattractant receptor-homologous molecule expressed on Th2 cells (CRTh2) antagonist[1]. CRTh2 antagonist 3 (compound 4) also enhances the activity of PDK1 toward a short peptide substrate, with an EC50 of 2 μM and a Kd of 8.4 μM. CRTh2 antagonist 3 has the potential for cardiovascular inflammation[2].

  • CAS Number: 312928-72-6
  • MF: C19H20N2O3S
  • MW: 356.44
  • Catalog: PDK-1
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

MRT 199665

MRT 199665 is a potent salt-inducible kinases (SIKs) inhibitor with IC50 of 110, 12, 43 nM for SIK1,2,3 respectively; also inhibits AMPKα1/α2 (both IC50=10 nM), MARK1/2/3/4 (both IC50=2 nM), NUAK1/2 (IC50=3/120 nM), and MELK (IC50=29 nM); elevates IL-10 production by inducing the dephosphorylation of CREB-regulated transcriptional coactivator 3 (CRTC3), increases LPS-stimulated IL-10 production and greatly suppressed proinflammatory cytokine secretion (IL-6, IL-12, and TNF) in macrophages.

  • CAS Number: 1456858-57-3
  • MF: C18H19N3O4S
  • MW: 373.43
  • Catalog: Apoptosis
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

PKI-179 hydrochloride

PKI-179 hydrochloride is a potent and orally active dual PI3K/mTOR inhibitor, with IC50s of 8 nM, 24 nM, 74 nM, 77 nM, and 0.42 nM for PI3K-α, PI3K-β, PI3K-γ, PI3K-δ and mTOR, respectively. PKI-179 hydrochloride also exhibits activity over E545K and H1047R, with IC50s of 14 nM and 11 nM, respectively. PKI-179 hydrochloride shows anti-tumor activity in vivo[1][2].

  • CAS Number: 1463510-35-1
  • MF: C25H29ClN8O3
  • MW: 525.00
  • Catalog: mTOR
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Vaccarin

Vaccarin is an active flavonoid glycoside associated with various biological functions. Vaccarin significantly promote wound healing and endothelial cells and fibroblasts proliferation in the wound site. Vaccarin ameliorates insulin resistance and steatosis by activating the AMPK signaling pathway[1][2].

  • CAS Number: 53452-16-7
  • MF: C32H38O19
  • MW: 726.633
  • Catalog: AMPK
  • Density: 1.8±0.1 g/cm3
  • Boiling Point: 1088.1±65.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 345.6±27.8 °C

HTH 01-015

HTH-01-015 is a selective NUAK1 inhibitor (IC50 is 100 nM). HTH-01-015 inhibits NUAK1 with >100-fold higher potency than NUAK2 (IC50 of >10 μM).

  • CAS Number: 1613724-42-7
  • MF: C26H28N8O
  • MW: 468.553
  • Catalog: AMPK
  • Density: 1.4±0.1 g/cm3
  • Boiling Point: 759.6±70.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 413.2±35.7 °C

FAP-PI3KI1

FAP-PI3KI1 is a fibroblast-activated protein (FAP)-targeted PI3K inhibitor that selectively targets FAP-expressing human idiopathic pulmonary fibrosis (IPF) cells and effectively inhibits collagen synthesis and reduces collagen deposition[1].

  • CAS Number: 2415941-98-7
  • MF: C52H48F4N10O12S3
  • MW: 1177.19
  • Catalog: PI3K
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

PI3K-IN-10

PI3K-IN-10 is a potent pan-PI3K inhibitor as a benzimidazole derivative, compound 332, extracted from patent WO2018057808A1[1].

  • CAS Number: 2211922-64-2
  • MF: C23H19ClN6O
  • MW: 430.89
  • Catalog: PI3K
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

PI3Kα-IN-11

PI3Kα-IN-11 is a potent PI3Kα inhibitor with anticancer activity[1].

  • CAS Number: 300803-79-6
  • MF: C17H14N2O3
  • MW: 294.30
  • Catalog: PI3K
  • Density: 1.4±0.1 g/cm3
  • Boiling Point: 622.4±55.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 330.2±31.5 °C

LY3023414

LY3023414 potently and selectively inhibits class I PI3K isoforms, DNA-PK, and mTORC1/2 with IC50s of 6.07 nM, 77.6 nM, 38 nM, 23.8 nM, 4.24 nM and 165 nM for PI3Kα, PI3Kβ, PI3Kδ, PI3Kγ, DNA-PK and mTOR, respectively. LY3023414 potently inhibits mTORC1/2 at low nanomolar concentrations.

  • CAS Number: 1386874-06-1
  • MF: C23H26N4O3
  • MW: 406.478
  • Catalog: DNA-PK
  • Density: 1.2±0.1 g/cm3
  • Boiling Point: 591.7±50.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 311.6±30.1 °C

Adropin (34-76) (human, mouse, rat) trifluoroacetate salt

Adropin (34-76) (human, mouse, rat) regulates fuel selection preferences in skeletal muscle. Adropin increases insulin-induced Akt phosphorylation and cell-surface expression of GLUT4, in diet-induced obesity (DIO) mouse.

  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

BIO

GSK 3 Inhibitor IX (6-Bromoindirubin-3'-oxime; BIO) is a potent, selective, reversible and ATP-competitive inhibitor of GSK-3α/β and CDK1-cyclinB complex with IC50s of 5 nM/320 nM/80 nM for (GSK-3α/β)/CDK1/CDK5, respectively.

  • CAS Number: 667463-62-9
  • MF: C16H10BrN3O2
  • MW: 356.173
  • Catalog: GSK-3
  • Density: 1.8±0.1 g/cm3
  • Boiling Point: 554.3±50.0 °C at 760 mmHg
  • Melting Point: 300°C(lit.)
  • Flash Point: 289.0±30.1 °C

PF-06679142

PF-06679142 is a novel potent, orally active, α1β1γ1/α2β1γ1-isoform selective AMPK activator with Kd of 14 nM, EC50 of 22 nM for α1β1γ1-AMPK, shows minimal activity at the β2-containing isoforms (α1β2γ1, α2β2γ1, α2β2γ3); demonstrates in vivo target engagement experiment in ZSF-1 rats, an obese/diabetic rodent model of diabetic nephropathy.

  • CAS Number: 1467059-66-0
  • MF: C20H17F2NO3
  • MW: 357.357
  • Catalog: AMPK
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

USP10 inhibitor Wu-5

USP10 inhibitor Wu-5 (Wu-5) is a novel small molecule USP10 inhibitor inducing the degradation of FLT3-mutated protein, directly interacts and inactivates USP10, the deubiquitinase for FLT3-ITD in vitro (IC50=8.3 uM) and in FLT3-ITD-positive AML cells.Wu-5 selectively inhibited the viability of FLT3 inhibitor-sensitive (MV4-11, Molm13) and -resistant (MV4-11R) FLT3-ITD-positive AML cells with IC50 of 3.794, 5.056, and 8.386 uM, respectively.Wu-5 (1-10 μM) dose-dependently induced apoptosis of MV4-11, Molm13, and MV4-11R cells through the proteasome-mediated degradation of FLT3-ITD.Combined treatment of Wu-5 and crenolanib produced synergistic cell death in FLT3-ITD-positive cells via the reduction of both FLT3 and AMPKα proteins.

  • CAS Number: 2630378-05-9
  • MF: C15H13NO7S
  • MW: 351.329
  • Catalog: Apoptosis
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

740 Y-P

740 Y-P (PDGFR 740Y-P) is a potent and cell permeable PI3K activator.

  • CAS Number: 1236188-16-1
  • MF: C141H222N43O39PS3
  • MW: 3270.7
  • Catalog: Peptides
  • Density: 1.5±0.1 g/cm3
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

PI3K/mTOR Inhibitor-13

PI3K/mTOR Inhibitor-13 is an orally active dual inhibitor of phosphoinositol 3-kinase (PI3K) and mTOR kinase. PI3K/mTOR Inhibitor-13 has potential applications in sexual diseases, solid tumor and idiopathic pulmonary fibrosis (IPF)[1][2].

  • CAS Number: 1621718-37-3
  • MF: C20H13F2N5O3S
  • MW: 441.41
  • Catalog: mTOR
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

AR-A014418

AR-A014418 is a potent, selective and ATP-competitive GSK3β inhibitor with an IC50 of 104 nM。

  • CAS Number: 487021-52-3
  • MF: C12H12N4O4S
  • MW: 308.313
  • Catalog: GSK-3
  • Density: 1.5±0.1 g/cm3
  • Boiling Point: N/A
  • Melting Point: 208-210?C (dec.)
  • Flash Point: N/A

ATR-IN-8

ATR-IN-8 is a potent inhibitor of ATR. ATR is a key enzyme in the homologous recombination repair pathway and belongs to the PIKK family. ATR-IN-8 has the potential for the research of cancer diseases (extracted from patent WO2021143821A1, compound 3)[1].

  • CAS Number: 2672511-20-3
  • MF: C20H22N6O2S
  • MW: 410.49
  • Catalog: ATM/ATR
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Buformin hydrochloride

Buformin hydrochloride (1-Butylbiguanide hydrochloride) is a potent and orally active biguanide antidiabetic agent, an AMPK activator. Buformin hydrochloride decreases hepatic gluconeogenesis and lowers blood glucose production in vivo. Buformin hydrochloride also has anti-cancer activities and is applied in cancer study (such as, cervical cancer and breast cancer, et al)[1].

  • CAS Number: 1190-53-0
  • MF: C6H16ClN5
  • MW: 193.67800
  • Catalog: AMPK
  • Density: N/A
  • Boiling Point: 322.7ºC at 760 mmHg
  • Melting Point: 174-177ºC
  • Flash Point: 148.9ºC

AMPK activator 12

AMPK activator 12 (compound 21) is a potent AMPK activator and GDF15 inducer. AMPK activator 12 increases GDF15 protein levels in human hepatic cells[1].

  • CAS Number: 431920-24-0
  • MF: C23H24BrNO2
  • MW: 426.35
  • Catalog: AMPK
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Erucic acid

Erucic acid, a monounsaturated fatty acid (MUFA), is isolated from the seed of Raphanus sativus L. Erucic acid can readily cross the blood-brain barrier (BBB), it has been reported to normalize the accumulation of very long-chain fatty acids in the brain. Erucic acid can improve cognitive impairment and be effective against dementia[1].

  • CAS Number: 112-86-7
  • MF: C22H42O2
  • MW: 338.568
  • Catalog: PI3K
  • Density: 0.9±0.1 g/cm3
  • Boiling Point: 386.1±0.0 °C at 760 mmHg
  • Melting Point: 28-32 °C(lit.)
  • Flash Point: 349.9±15.2 °C

Vacuolin-1

A potent PIKfyve inhibitor that inhibits autophagy by impairing lysosomal maturation, potently and reversibly inhibits autophagosome-lysosome fusion by activating RAB5A; blocks the Ca(2+)-dependent exocytosis of lysosomes induced by ionomycin or plasma membrane wounding, without affecting the process of resealing, induces the formation of giant vacuoles.

  • CAS Number: 351986-85-1
  • MF: C26H24IN7O
  • MW: 577.419
  • Catalog: Autophagy
  • Density: 1.5±0.1 g/cm3
  • Boiling Point: 719.7±70.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 389.1±35.7 °C

JNJ-47117096 hydrochloride

JNJ-47117096 hydrochloride is potent and selective MELK inhibitor, with an IC50 of 23 nM, also effectively inhibits Flt3, with an IC50 of 18 nM.

  • CAS Number: 1610536-69-0
  • MF: C21H23ClN4O2
  • MW: 398.886
  • Catalog: MELK
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

IBL-302

IBL-302 (AMU302) is an orally available dual-signaling inhibitor of PIM and PI3K/AKT/mTOR with activity against breast cancer and neuroblastoma. IBL-302 demonstrated in vivo efficacy in a nude mouse xenograft model, inhibiting trastuzumab (HY-P9907) resistance challenges. IBL-302 also enhances the effects of common cytotoxic chemotherapy drugs cisplatin (HY-17394), doxorubicin (HY-15142A), and etoposide (HY-13629)[1][2][3].

  • CAS Number: 1414455-21-2
  • MF: C25H18FN5O4S3
  • MW: 567.63
  • Catalog: Pim
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A