Uric Acid

Names

[ CAS No. ]:
69-93-2

[ Name ]:
Uric Acid

[Synonym ]:
2,6,8-trioxypurine
1H-Purine-2,6,8 (3H)-trione, 7,9-dihydro-
1H-Purine-2,6,8(3H)-trione, 7,9-dihydro-
[14C]-Uric acid
2,6,8-trioxopurine
EINECS 200-720-7
7,9-Dihydro-1H-purine-2,6,8(3H)-trione
1H-Purine-2,6,8-triol
urate
[3H]-Uric acid
2,6,8-trihydroxypurine
Uric Acid
Uric acid (8CI)
MFCD00005712
1H-Purine-2,6,8(3H)-trione, 7,9-dihydro- (9CI)
1H-Purine-2,6,8(3H)-trione,7,9-dihydro
Lithic acid
7,9-dihydro-3H-purine-2,6,8-trione

Biological Activity

[Description]:

Uric acid is an endogenous antioxidant that scavenges reactive oxygen species (ROS) including singlet oxygen, oxygen radicals, and peroxynitrite.

[Related Catalog]:

Research Areas >> Inflammation/Immunology

Natural Products >> Others

[Target]

Human Endogenous Metabolite

[In Vitro]

Uric acid is an endogenous antioxidant that scavenges reactive oxygen species (ROS) including singlet oxygen, oxygen radicals, and peroxynitrite. Incubation with indomethacin significantly increases malondialdehyde (MDA) levels in Caco-2 cells compare to those not treated indomethacin. Incubation with both indomethacin and Uric acid significantly decreases MDA levels compare to those grown in the presence of indomethacin alone. Co-treatment of cells with indomethacin and Uric acid significantly decreases ROS levels compare to those in cells incubated with indomethacin alone. Cell viability in Caco-2 cells treated with both indomethacin and Uric acid is higher than that in cells treated with indomethacin alone. Uric acid has a protective effect on indomethacin-induced intestinal cell changes through its antioxidant activity[1].

[In Vivo]

When mice treated with indomethacin are concurrently administered Uric acid orally, ulcer areas are significantly reduced, in a Uric acid dose-dependent manner. Indomethacin increases the ratio of crypt depth to villous height in the ileum, while the ratio is significantly lower when mice are concurrently administered Uric acid orally. Administration of indomethacin also increases the histopathological score of tissue damage in the small intestine, while mice concurrently administered Uric acid orally has a significantly lower histopathological score. The ileal levels of malondialdehyde (MDA) in indomethacin-induced enteropathy model mice orally administered Uric acid are also significantly lower than the levels in mice administered indomethacin alone[1].

[Cell Assay]

Human colon carcinoma cells (Caco-2) are used and grown in Eagle’s minimum essential medium supplemented with 10% FBS, 1% non-essential amino acid mixture, and 1% Pen-Strep at 37°C in a humidified atmosphere with 5% CO2. Caco-2 cells are incubated with indomethacin in the presence or absence of Uric acid for 24 or 48 hours[1].

[Animal admin]

To evaluate the effect of oral administration of Uric acid on NSAID-induced enteropathy, mice are given Uric acid (2.5, 10, 25, 100, or 250 mg/kg body weight) suspended in 0.5% carboxymethyl cellulose orally at 30 minutes before, and 12 hours after indomethacin or vehicle treatment. To evaluate the effect of intraperitoneal administration of inosinic acid plus potassium oxonate on NSAID-induced enteropathy, mice are given inosinic acid (500 mg/kg body weight) plus potassium oxonate (250 mg/kg body weight) 24 intraperitoneally at 30 minutes before, and 12 hours after indomethacin or vehicle treatment[1].

[References]

[1]. Yasutake Y, et al. Uric acid ameliorates indomethacin-induced enteropathy in mice through its antioxidant activity. J Gastroenterol Hepatol. 2017 Nov;32(11):1839-1845.

[Related Small Molecules]

Chemical & Physical Properties

[ Density]:
1.9±0.1 g/cm3

[ Boiling Point ]:
863ºC at 760 mmHg

[ Melting Point ]:
>300 °C(lit.)

[ Molecular Formula ]:
C₅H₄N₄O₃

[ Molecular Weight ]:
168.110

[ Flash Point ]:
475.7ºC

[ Exact Mass ]:
168.028336

[ PSA ]:
114.37000

[ LogP ]:
-1.08

[ Index of Refraction ]:
1.721

[ Storage condition ]:
Store at RT.

[ Stability ]:
Stable. Incompatible with acids, bases, oxidising agents.

MSDS

Click to get detail MSDS

Toxicological Information

CHEMICAL IDENTIFICATION

RTECS NUMBER :: YU7050080

CHEMICAL NAME :: Uric acid

CAS REGISTRY NUMBER :: 69-93-2

LAST UPDATED :: 199710

DATA ITEMS CITED :: 7

MOLECULAR FORMULA :: C5-H4-N4-O3

MOLECULAR WEIGHT :: 168.13

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

TYPE OF TEST :: TDLo - Lowest published toxic dose

ROUTE OF EXPOSURE :: Oral

DOSE :: 5040 mg/kg

SEX/DURATION :: male 4 week(s) pre-mating

TOXIC EFFECTS :: Reproductive - Paternal Effects - testes, epididymis, sperm duct

MUTATION DATA

TYPE OF TEST :: Mutation test systems - not otherwise specified

TEST SYSTEM :: Human Lymphocyte

DOSE/DURATION :: 10 mmol/L

REFERENCE :: CYTBAI Cytobios. (Faculty Press, 88 Regent St., Cambridge, UK) V.1- 1969- Volume(issue)/page/year: 4,87,1971 *** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA *** NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA : NOHS - National Occupational Hazard Survey (1974) NOHS Hazard Code - 82048 No. of Facilities: 246 (estimated) No. of Industries: 5 No. of Occupations: 4 No. of Employees: 2320 (estimated) NOES - National Occupational Exposure Survey (1983) NOES Hazard Code - 82048 No. of Facilities: 1210 (estimated) No. of Industries: 5 No. of Occupations: 8 No. of Employees: 17397 (estimated) No. of Female Employees: 12409 (estimated)

Safety Information

[ Personal Protective Equipment ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ Hazard Codes ]:
Xi

[ Risk Phrases ]:
R33

[ Safety Phrases ]:
S24/25

[ RIDADR ]:
NONH for all modes of transport

[ WGK Germany ]:
3

[ RTECS ]:
YU7050080

[ HS Code ]:
2933990090

Synthetic Route

Click to get detail synthetic route

Precursor & DownStream

Precursor

DownStream

Customs

[ HS Code ]: 2933990090

[ Summary ]:
2933990090. heterocyclic compounds with nitrogen hetero-atom(s) only. VAT:17.0%. Tax rebate rate:13.0%. . MFN tariff:6.5%. General tariff:20.0%

Articles

Graphene modified glassy carbon sensor for the determination of aspirin metabolites in human biological samples.

Talanta 143 , 328-34, (2015)

A graphene modified glassy carbon (GR/GCE) sensor has been developed for the determination of aspirin metabolites 2,3- and 2,5-dihydroxybenzoic acids (2,3- and 2,5-DHB). The modified sensor was charac...

Hepatorenal protective effect of Antistax(®) against chemically-induced toxicity.

Pharmacogn. Mag. 11 , S173-81, (2015)

Antioxidant natural products and chemoprevention are considered nowadays as an effective approach against health various disorders and diseases induced by oxidative stress or free radicals.The aim of ...

Facile and controllable one-step fabrication of molecularly imprinted polymer membrane by magnetic field directed self-assembly for electrochemical sensing of glutathione.

Anal. Chim. Acta 886 , 37-47, (2015)

Based on magnetic field directed self-assembly (MDSA) of the ternary Fe3O4@PANI/rGO nanocomposites, a facile and controllable molecularly imprinted electrochemical sensor (MIES) was fabricated through...

Related Compounds

The content on this webpage is sourced from various professional data sources. If you have any questions or concerns regarding the content, please feel free to contact service1@chemsrc.com.