MCH (salmon)

Modify Date: 2024-01-02 17:30:37

MCH (salmon) structure	Common Name	MCH (salmon)
	CAS Number	87218-84-6	Molecular Weight	2099.48000
	Density	N/A	Boiling Point	N/A
	Molecular Formula	C₈₉H₁₃₉N₂₇O₂₄S₄	Melting Point	N/A
	MSDS	Chinese USA	Flash Point	N/A

Use of MCH (salmon)

Melanin Concentrating Hormone, salmon is a 19-amino-acid neuropeptide initially identified in the pituitary gland of teleost fish, which regulates food intake, energy balance, sleep state, and the cardiovascular system. Melanin-concentrating hormone is a ligand for an orphan G protein-coupled receptor (SLC-1/GPR24) and MCHR2.

Name	Melanin Concentrating Hormone salmon
Synonym	More Synonyms

Description	Melanin Concentrating Hormone, salmon is a 19-amino-acid neuropeptide initially identified in the pituitary gland of teleost fish, which regulates food intake, energy balance, sleep state, and the cardiovascular system. Melanin-concentrating hormone is a ligand for an orphan G protein-coupled receptor (SLC-1/GPR24) and MCHR2.
Related Catalog	Signaling Pathways >> GPCR/G Protein >> MCHR1 (GPR24) Research Areas >> Cardiovascular Disease Research Areas >> Metabolic Disease Research Areas >> Neurological Disease Peptides
Target	SLC-1/GPR24, MCHR2[1]
In Vitro	An orphan G protein-coupled receptor (SLC-1/GPR24) has been identified as a receptor for MCH (MCHR1). MCHR2 has higher protein sequence homology to MCHR1 than any other G protein-coupled receptor. MCHR2 is specifically activated by nanomolar concentrations of MCH, binds to MCH with high affinity, and signals through Gq protein[1].
In Vivo	Melanin Concentrating Hormone stimulates appetite. Continuous infusion of Melanin Concentrating Hormone into the ventricular system increases food intake for 7-8 days[2]. Intracerebroventricular infusion of Melanin Concentrating Hormone (10 μg/day) causes a slight but significant increase in body weight in mice maintained on the regular diet. Chronic stimulation of the brain Melanin Concentrating Hormone system could cause obesity in mice[3].
Kinase Assay	Binding of 125I-labeled Melanin Concentrating Hormone (MCH) to MCHR1 and MCHR2 is measured by filtration binding assay. Membranes (10 μg protein) from transiently transfected HEK293-MCHR1 and HEK293-MCHR2 cells are mixed with 0-9.8 nM 125I-labeled MCH in the binding buffer (50 mM Hepes/10 mM MgCl内容2/2 mM EGTA; protease inhibitors, 0.1% BSA, pH 7.6). After incubation for 1 h at room temperature, membrane-bound 125I-labeled MCH is separated from the free 125I-labeled MCH by filtration through a 96-well GF/B plate on a Packard Filtermate Cell Harvester and ished with ice-cold binding buffer supplemented with 80 mM NaCl. Eighty microliters of scintillation liquid is added, and the radioactivity is counted on a Packard Microplate Topcount[1].
Animal Admin	Rats[2] Both male Wistar and male Sprague-Dawley rats weighing between 310 and 370 g (age 8-11 weeks) are used in the study. All animals receive food which had the following dry weight composition: 64% carbohydrate, 22% protein, 4.3% fat, 4% cellulose and 5.5% ash. For chronic experiments, the rats are infused either with artificial CSF (0.5 μL/h) or MCH (8 μg/rat/day).Body weight and food intake are then recorded daily for 12 days[2]. Mice[3] Male C57BL/6J mice is prepared for measurement of spontaneous motor activity. Melanin Concentrating Hormone (10 μg/day) or the vehicle is infused for 14 days under the regular diet-fed condition. Motor activity is measured during the last 3 days of the 14-day infusion by an activity monitoring system in home cages[3].
References	[1]. An S, et al. Identification and characterization of a melanin-concentrating hormone receptor. Proc Natl Acad Sci U S A. 2001 Jun 19;98(13):7576-81. [2]. Della-Zuana O, et al. Acute and chronic administration of melanin-concentrating hormone enhances food intake and body weight in Wistar and Sprague-Dawley rats. Int J Obes Relat Metab Disord. 2002 Oct;26(10):1289-95. [3]. Gomori A, et al. Chronic intracerebroventricular infusion of MCH causes obesity in mice. Melanin-concentrating hormone. Am J Physiol Endocrinol Metab. 2003 Mar;284(3):E583-8. Epub 2002 Nov 26.

Molecular Formula	C₈₉H₁₃₉N₂₇O₂₄S₄
Molecular Weight	2099.48000
Exact Mass	2097.94000
PSA	937.88000
LogP	3.92200

Personal Protective Equipment	Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter
Safety Phrases	S22-S24/25
RIDADR	NONH for all modes of transport