Amyloid β-Protein (22-35)

Modify Date: 2024-01-03 18:28:33

Amyloid β-Protein (22-35) structure	Common Name	Amyloid β-Protein (22-35)
	CAS Number	144189-71-9	Molecular Weight	1403.60000
	Density	N/A	Boiling Point	N/A
	Molecular Formula	C₅₉H₁₀₂N₁₆O₂₁S	Melting Point	N/A
	MSDS	Chinese USA	Flash Point	N/A

Use of Amyloid β-Protein (22-35)

β-Amyloid (22-35) is a 14-aa peptide, shows aggregates and induces neurotoxicity in the hippocampal cells.

Name	Amyloid β-Protein Fragment 22-35
Synonym	More Synonyms

Description	β-Amyloid (22-35) is a 14-aa peptide, shows aggregates and induces neurotoxicity in the hippocampal cells.
Related Catalog	Signaling Pathways >> Neuronal Signaling >> Amyloid-β Research Areas >> Neurological Disease Peptides
Target	Amyloid-β[1]
In Vitro	β-Amyloid (22-35) shows significant cytotoxicity on the rat hippocampal neurons at 40 μg/mL, but exhibits no cytotoxic effect on the glial cells[1].
Cell Assay	Hippocampal cells are cultured in DMEM supplemented with 10% (v/v) precolostrum newborn calf serum in poly-D-lysine-coated 24-well culture plates at 37°C under a gas mixture of 95% air/5% CO2. After 6 days of culture, non-neuronal cell division is halted by 24 h exposure to 10-5 cytosine arabinoside. After washing cell layers with DMEM, cells are cultured with 1 mL of DMEM per well in the absence of serum for 5 days before experiment. Cells are treated for 3 days with β-Amyloid (22-35) or its amidated (C-terminus) derivative by addition of 20 μL of stock solution (2 mg/mL of either in betaine buffer, pH 8.5, consisting of 0.7% betaine and 20 mM NH4HCO3) to 1.0 mL medium, unless otherwise specified. Control cells are treated with the betaine buffer alone. Morphological change of cells is checked throughout the course of experiment with a phase-contrast microscope. Dead cells are checked by trypan blue staining. Cell injury is assessed by measuring lactate dehydrogenase (LDH) activity released into medium during 3 days of treatment of neurons with peptides[1] .
References	[1]. Takadera T, et al. Toxic effect of a beta-amyloid peptide (beta 22-35) on the hippocampal neuron and its prevention. Neurosci Lett. 1993 Oct 14;161(1):41-4.

Molecular Formula	C₅₉H₁₀₂N₁₆O₂₁S
Molecular Weight	1403.60000
Exact Mass	1402.71000
PSA	630.86000
LogP	0.68100
Storage condition	−20°C

Personal Protective Equipment	Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter
RIDADR	NONH for all modes of transport
WGK Germany	3

Toxic effect of a beta-amyloid peptide (beta 22-35) on the hippocampal neuron and its prevention.

Neurosci. Lett. 161 , 41, (1993)

A synthetic truncated beta-amyloid peptide, beta 22-35, was shown to have a cytotoxic effect on cultured neurons from the rat hippocampus in serum-free medium. The peptide formed aggregates and typica...

MFCD00236784

β-Amyloid (22-35),EDVGSNKGAIIGLM

β-Amyloid (22-35)

H-EDVLGSNKGAIIGLM-OH