Antimicrobial Agents and Chemotherapy 2009-12-01

Modifying culture conditions in chemical library screening identifies alternative inhibitors of mycobacteria.

Christopher H Miller, Shahista Nisa, Sandi Dempsey, Cameron Jack, Ronan O'Toole

Index: Antimicrob. Agents Chemother. 53 , 5279-83, (2009)

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Abstract

In this study, application of a dual absorbance/fluorescence assay to a chemical library screen identified several previously unknown inhibitors of mycobacteria. In addition, growth conditions had a significant effect on the activity profile of the library. Some inhibitors such as Se-methylselenocysteine were detected only when screening was performed under nutrient-limited culture conditions as opposed to nutrient-rich culture conditions. We propose that multiple culture condition library screening is required for complete inhibitory profiling and for maximal antimycobacterial compound detection.