Liquid chromatography is one of the most important methods for microanalysis of current constituents. The two phases in the chromatographic analysis refer to a stationary phase having a large specific surface area and a mobile phase carrying a mixture to be separated flowing through the stationary phase. A liquid phase liquid using a liquid as a mobile phase. Liquid chromatography is also known as "liquid chromatography." A chromatographic method in which the mobile phase used is a liquid. Since the liquid in the column is more resistant to gas than the gas, in order to increase the analysis speed, a high pressure infusion method is usually used, so it is also called "high pressure liquid chromatography". According to the different stationary phases, it can be divided into liquid-solid chromatography and liquid-liquid chromatography; depending on the type of equilibrium between the stationary phase and the mobile phase, it can be divided into adsorption chromatography, partition chromatography, ion exchange chromatography and gel chromatography. Used for separation and analysis of organic, inorganic and polymeric liquids. [A brief history] The Russian botanist MSTswett first used column chromatography to separate plant pigments in 1906. It is the oldest one in liquid chromatography and has developed into various kinds in the past 30 years. Chromatographic separation is an important part of analytical chemistry. 1 Classical liquid chromatography: the mobile phase flows downward from the upper end of the column tube by its own gravity, and the effluent is collected one by one at the outlet of the lower part of the column, and then measured by other methods, the analysis speed is slow and the efficiency is low; 2 thin layer chromatography : Appeared around the 1940s, easy to operate, improved analysis speed, but poor repeatability, quantitative difficulty; 3 high performance liquid chromatography: in the early 1960s, JCGidelings modified the gas chromatography theory for Liquid chromatography lays the foundation for its modernization. Since then, high-pressure pumps, high-efficiency stationary phases and high-sensitivity detectors have been used in technology, and developed into high-performance liquid chromatography with fast analysis speed, high separation efficiency and automatic operation. In 1971, RAHenry et al first used it for pesticide analysis. [High Performance Liquid Chromatography] High performance liquid chromatography is one of the most important separation methods in modern analytical chemistry. It originated from the classical liquid chromatography method. The basic method is to use a single solvent with a certain polarity or a mixed solution of different proportions as the mobile phase, and pump the mobile phase into the column with the filler, and inject it into the test. After the product is taken into the column by the mobile phase for separation, the components are successively entered into the detector, and the chromatogram or data processing is recorded by a recorder or a data processing device to obtain a measurement result. Due to the application of various characteristics of particulate filler and pressurized liquid mobile phase, the method has the characteristics of high separation performance and fast analysis speed. High performance liquid chromatography is suitable for the analytical determination of pharmaceuticals that can be separated on a specific filler column, especially for the determination of multi-component drugs, impurity inspection and determination of macromolecular substances. Some drugs need to be derivatized before or after chromatographic separation to be separated or detected. Commonly used column packing agents are: silica gel for normal phase chromatography; chemically bonded stationary phase, which can be used for reversed phase or normal phase chromatography depending on the bonded groups; ion exchange packing for ion exchange chromatography; Large pore packing for exclusion chromatography. High performance liquid chromatography consists essentially of pumps, injectors, columns, detectors, and chromatographic data processing systems. The most commonly used detectors are variable wavelength ultraviolet and visible light detectors, and other detectors such as refractive index detectors and evaporative light scattering detectors. The collection and processing of chromatographic information is often performed using an integrator or data workstation. Gradient elution can be achieved by two pumps or a single pump plus a proportional valve.


Amino compound >
Oxy-containing amino compound Cycloalkylamines, aromatic monoamines, aromatic polyamines and derivatives and salts thereof Acyclic monoamines, polyamines and their derivatives and salts Amide compound Sulfonic acid amino compound
Alcohols, phenols, phenolic compounds and derivatives >
2-cycloalcohol Halogenated, sulfonated, nitrated or nitrosated derivatives of alcohols Extremely halogenated, sulfonated, nitrated or nitrosated derivatives of phenols Phenol and its halogenated, sulfonated, nitrated or nitrosated derivatives Acyclic alcohol
Nitrogen-containing compound
Nitrile compound
Organic derivative of hydrazine or hydrazine
Terpenoid
Ether compounds and their derivatives >
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Aldehyde
Carboxylic compounds and derivatives >
Cyclic carboxylic acid Halogenation, sulfonation, nitration or nitrosation of carboxylic acids Halogenation, sulfonation, nitration or nitration of carboxylic anhydrides Carboxylic acid halide Carboxylic esters and their derivatives Salt of carboxylic acid ester and its derivatives Acyclic carboxylic acid
Hydrocarbon compounds and their derivatives >
Aromatic hydrocarbon Cyclic hydrocarbon Hydrocarbon sulfonate Hydrocarbon halide Hydrocarbon nitrite Acyclic hydrocarbon
Ketone compound
Alkyl ureas and their derivatives and salts
Inorganic acid ester
Heterocyclic compound
Diazo, azo or azo compound
Organosilicon compound
Organometallic compound >
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Organic sulfur compound
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Organometallic salt
Organic fluorine compound >
Fluorobenzoic acid series Fluorobenzonitrile series Fluorobenzaldehyde series Fluorobenzyl alcohol series Fluoroanisole series Fluoroaniline series Fluorophenylacetic acid series Fluorophenol series Fluorobenzoic acid series Fluoronitrobenzene series Fluoropyridine series Potassium fluoroborate series Fluorobenzyl alcohol series Fluorotoluene series Fluorine red series Fluoroethane series Fluoropropane series
Respiratory medication >
Asthma Other respiratory medications Peony Antitussive
Hormone and endocrine-regulating drugs >
Birth control pills Pituitary hormone Estrogen and progestogen drugs Adrenocorticotropic drugs Gonadotropin Thyroid hormone and antithyroid drugs other Prostaglandins Adrenal corticosteroids Androgen and anabolic hormone drugs Pancreatic hormones and other blood sugar regulating drugs Treatment of male erectile dysfunction drugs
Antipyretic analgesics >
Non-steroidal anti-inflammatory drugs Antipyretic and analgesic Anti-migraine drug Anti-gout medicine Analgesic
Antiallergic >
Allergic reaction medium blocker Antihistamine
Antiparasitic drug >
Anti-amebiasis and anti-trichomoniasis drugs Antimalarial Resistance to filariasis and anti-leishmaniasis Anti-fungal medicine Anthelmintic Antiprotozoal Anthelmintic
Antibiotics >
Beta-lactamase inhibitor Aminoglycoside Macrolide drug Peptide Polyene Rifamycin Lincosamide Other antibiotics Penicillin Tetracycline Cephalosporin Amide alcohol Agricultural antibiotics
Antineoplastic agents >
Hormone antineoplastic agents Antimetabolite antineoplastic Antibiotic antineoplastic agents Anti-tumor adjuvant Other antineoplastic agents Natural source antineoplastic agents Alkylating agent Tinic antineoplastic agents Idiopathic pulmonary fibrosis (IPF) treatment
Anesthetic Agents >
Skeletal muscle relaxant Local anesthetics General anesthetics
Urinary system medication >
Diuretic Diabetes insipidus medication Other urinary system medication Dehydrated medicine
Other chemicals >
other Health and epidemic prevention drugs
Synthetic anti-infective drugs >
Sulfonamides and synergists Antiviral drugs Anti-tuberculosis leprosy Antihelium drug Antifungal drugs Quinolone Natural source anti-infectives Disinfectant antiseptic Nitrofuran
Nervous system medication >
2 sedative and hypnotics Antiepileptic and anticonvulsant Anxiolytic Antipsychotic Antidepressant, manic Anti-shock palsy Brain metabolism regulating drug Cholinergic Other nervous system medication Central stimulant Parkinson's syndrome
Water, electrolyte and acid-base balance regulator >
Electrolyte balance regulator Glucose drug Acid-base balance regulator
Vitamins and minerals >
Trace elements and mineral drugs Vitamin AD drugs Vitamin B drugs Vitamin C drugs Nutritional health medicine
Digestive system medication >
Liver disease medication Acid and gastric mucosal protective drugs Biliary medicine Other digestive system medication Appetite suppressant and other diet pills Gastrointestinal drug Gastrointestinal expectorant Laxative antidiarrheal Inhibition of gastric acid secretion Antiemetic emetic Digestive drug
Blood system medication >
Leukocyte proliferative drug Platelet proliferative drug Anticoagulant and antiplatelet drugs Anti-anemia drug Blood volume expander Hemostatic drug
Circulatory system medication >
Regulating blood lipids Prevention and treatment of angina pectoris Calcium channel blocker Anti-congestive heart failure medicine Antihypertensive drug Antiarrhythmic drug Anti-shock vasoactive drug Other circulatory system medication Angiotensin converting enzyme and receptor inhibitor Peripheral vasodilator Beta-adrenergic receptor blocker Treatment of chronic cardiac insufficiency Anti-atherosclerosis
Diagnostic medication >
Organ function test agent Image inspection medication
Special medicine >
Radioisotope Antidote Stomatology medication Dermatology medication Surgical medication Ophthalmic medication
Drugs that affect tissue metabolism
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Immune function drug >
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Veterinary drug raw materials
Inhibitor
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Nucleic acid drug
Enzymes and coenzymes
Inhibitor >
Immune inhibitor Protein tyrosine kinase Neuronal Signaling PI3K/Akt/mTOR inhibitor (PI3K/Akt/mTOR) Metabolism Cell Cycle Epigenetics Cytoskeletal Signaling Angiogenesis DNA damage Proteases Apoptosis Mitogen-activated protein kinase (MAPK) Endocrinology & Hormones G protein coupled receptor(GPCR & G Protein) Transmembrane Transporters Tyrosine protein kinase/signal transducer and transcriptional activator inhibitor (JAK/STAT) TGF-beta/Smad signaling pathway inhibitor (TGF-beta/Smad) Stem Cells & Wnt Signaling Paths (Stem Cells & Wnt) Nuclear transcription factor (NF-κB) Ubiquitin inhibitor Microbiology Autophagy lysosome (Autophagy)
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Amino acids and their derivatives >
Arginine derivatives Asparagine derivatives Aspartic acid derivative Cysteine derivative Glutamine derivative Glutamic acid derivative Glycine derivatives Histidine derivative Isoleucine derivative Leucine derivative Lysine derivative Methionine derivatives Phenylalanine derivatives Proline derivatives Serine derivative Threonine derivative Tryptophan derivatives Tyrosine derivatives Proline derivatives Alanine derivatives Methionine derivatives BOC-amino acid Cbz-amino acid FMOC-amino acid --amino acid Other protected amino acids Amino alcohol derivative Amino acid salt Ester amino acid Other amino acid derivatives Natural amino acids and their derivatives
Carbohydrate >
Monosaccharide Oligosaccharide Polysaccharide Double sugar
Biochemical reagent >
Biological dye Acid-base indicator Adjuvant
Nucleoside drugs >
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Condensing agent
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Protein research
antibody >
Antibody Enzyme secondary antibody Apoptosis and autophagy Cell metabolism Chromatin / epigenetic / cell cycle Cytoskeleton/extracellular matrix MAPK and PI3K/Akt pathway Neuroscience Immunity and inflammation PKC, calcium ion and lipid signaling pathway Stem cells, development and differentiation Protein translation, folding and degradation Other categories of primary antibodies
  • CAS Number: 446044-45-7
  • MF: C23H19NO4
  • MW: 373.401
  • Catalog:HPLC labeling reagent
  • Density: 1.4±0.1 g/cm3
  • Boiling Point: 631.4±48.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 335.6±29.6 °C
  • CAS Number: 143112-51-0
  • MF: C10H11N5O3
  • MW: 249.226
  • Catalog:HPLC labeling reagent
  • Density: 1.5±0.1 g/cm3
  • Boiling Point: 469.7±55.0 °C at 760 mmHg
  • Melting Point: -190ºC (dec.)
  • Flash Point: 237.9±31.5 °C
  • CAS Number: 139332-66-4
  • MF: C10H11N5O3
  • MW: 249.226
  • Catalog:HPLC labeling reagent
  • Density: 1.4±0.1 g/cm3
  • Boiling Point: 475.7±55.0 °C at 760 mmHg
  • Melting Point: 208-212 °C
  • Flash Point: 241.5±31.5 °C
  • CAS Number: 143112-52-1
  • MF: C10H11N5O3
  • MW: 249.226
  • Catalog:HPLC labeling reagent
  • Density: 1.5±0.1 g/cm3
  • Boiling Point: 469.7±55.0 °C at 760 mmHg
  • Melting Point: 178-184ºC
  • Flash Point: 237.9±31.5 °C
  • CAS Number: 107807-39-6
  • MF: C9H5N3O2S
  • MW: 219.220
  • Catalog:HPLC labeling reagent
  • Density: 1.6±0.1 g/cm3
  • Boiling Point: N/A
  • Melting Point: 41ºC
  • Flash Point: N/A
  • CAS Number: 3326-34-9
  • MF: C20H13NO5
  • MW: 347.321
  • Catalog:HPLC labeling reagent
  • Density: 1.6±0.1 g/cm3
  • Boiling Point: 695.3±55.0 °C at 760 mmHg
  • Melting Point: 223ºC
  • Flash Point: 374.3±31.5 °C
  • CAS Number: 1858255-08-9
  • MF: C18H23N5O7S
  • MW: 453.470
  • Catalog:HPLC labeling reagent
  • Density: 1.5±0.1 g/cm3
  • Boiling Point: 643.5±65.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 343.0±34.3 °C
  • CAS Number: 38183-12-9
  • MF: C17H10O4
  • MW: 278.259
  • Catalog:HPLC labeling reagent
  • Density: 1.4±0.1 g/cm3
  • Boiling Point: 550.3±50.0 °C at 760 mmHg
  • Melting Point: 153-157 °C(lit.)
  • Flash Point: 248.5±30.2 °C
  • CAS Number: 108-32-7
  • MF: C4H6O3
  • MW: 102.089
  • Catalog:Inorganic acid ester
  • Density: 1.2±0.1 g/cm3
  • Boiling Point: 241.7±0.0 °C at 760 mmHg
  • Melting Point: -49ºC
  • Flash Point: 132.2±0.0 °C
  • CAS Number: 68123-33-1
  • MF: C12H10N2O6
  • MW: 278.218
  • Catalog:HPLC labeling reagent
  • Density: 1.5±0.1 g/cm3
  • Boiling Point: 463.6±47.0 °C at 760 mmHg
  • Melting Point: 181ºC
  • Flash Point: 234.1±29.3 °C
  • CAS Number: 446044-44-6
  • MF: C23H19NO4
  • MW: 373.401
  • Catalog:HPLC labeling reagent
  • Density: 1.4±0.1 g/cm3
  • Boiling Point: 631.4±48.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 335.6±29.6 °C
  • CAS Number: 98358-90-8
  • MF: C8H8FN3O3S
  • MW: 245.231
  • Catalog:HPLC labeling reagent
  • Density: 1.5±0.1 g/cm3
  • Boiling Point: 360.9±52.0 °C at 760 mmHg
  • Melting Point: 123-128 °C
  • Flash Point: 172.0±30.7 °C
  • CAS Number: 179951-63-4
  • MF: C11H16N6O4S
  • MW: 328.348
  • Catalog:HPLC labeling reagent
  • Density: 1.476g/cm3
  • Boiling Point: 165.0 to 167.0 °C
  • Melting Point: 165.0 to 167.0 °C
  • Flash Point: N/A
  • CAS Number: 54455-34-4
  • MF: C9H5ClN2O4
  • MW: 240.600
  • Catalog:HPLC labeling reagent
  • Density: 1.6±0.1 g/cm3
  • Boiling Point: 397.4±27.0 °C at 760 mmHg
  • Melting Point: 153.0 to 157.0 °C
  • Flash Point: 194.1±23.7 °C
  • CAS Number: 156153-43-4
  • MF: C11H13ClN4O4S
  • MW: 332.763
  • Catalog:HPLC labeling reagent
  • Density: 1.5±0.1 g/cm3
  • Boiling Point: 483.1±55.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 245.9±31.5 °C
  • CAS Number: 114389-69-4
  • MF: C8H19NO2
  • MW: 161.24200
  • Catalog:Ion pair reagent for HPLC
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A
  • CAS Number: 7732-18-5
  • MF: H2O
  • MW: 18.015
  • Catalog:HPLC solvent
  • Density: 1.0±0.1 g/cm3
  • Boiling Point: 100.0±9.0 °C at 760 mmHg
  • Melting Point: 0 ºC
  • Flash Point: 100°C
  • CAS Number: 124522-09-4
  • MF: C10H8BrNO3
  • MW: 270.079
  • Catalog:HPLC labeling reagent
  • Density: 1.7±0.1 g/cm3
  • Boiling Point: 376.9±52.0 °C at 760 mmHg
  • Melting Point: 144-148ºC
  • Flash Point: 181.8±30.7 °C
  • CAS Number: 17683-09-9
  • MF: C14H15N3
  • MW: 225.289
  • Catalog:HPLC labeling reagent
  • Density: 1.1±0.1 g/cm3
  • Boiling Point: 335.0±45.0 °C at 760 mmHg
  • Melting Point: 77ºC
  • Flash Point: 156.4±28.7 °C
  • CAS Number: 642995-15-1
  • MF: C19H17NO4
  • MW: 323.343
  • Catalog:HPLC labeling reagent
  • Density: 1.4±0.1 g/cm3
  • Boiling Point: 554.6±43.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 289.2±28.2 °C
  • CAS Number: 18861-78-4
  • MF: C21H11NO5S
  • MW: 389.381
  • Catalog:HPLC labeling reagent
  • Density: 1.5±0.1 g/cm3
  • Boiling Point: 719.1±60.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 388.7±32.9 °C
  • CAS Number: 72565-41-4
  • MF: C14H17N5O2S
  • MW: 319.382
  • Catalog:HPLC labeling reagent
  • Density: 1.3±0.1 g/cm3
  • Boiling Point: 540.9±60.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 280.9±32.9 °C
  • CAS Number: 642995-16-2
  • MF: C19H17NO4
  • MW: 323.343
  • Catalog:HPLC labeling reagent
  • Density: 1.4±0.1 g/cm3
  • Boiling Point: 554.6±43.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 289.2±28.2 °C
  • CAS Number: 189373-41-9
  • MF: C10H15N5O3S
  • MW: 285.323
  • Catalog:HPLC labeling reagent
  • Density: 1.4±0.1 g/cm3
  • Boiling Point: 491.5±55.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 251.0±31.5 °C
  • CAS Number: 49759-20-8
  • MF: C17H10N2O2
  • MW: 274.273
  • Catalog:HPLC labeling reagent
  • Density: 1.4±0.1 g/cm3
  • Boiling Point: 508.2±23.0 °C at 760 mmHg
  • Melting Point: 188-190°C
  • Flash Point: 261.2±22.6 °C
  • CAS Number: 131467-86-2
  • MF: C8H11N5O3S
  • MW: 257.270
  • Catalog:HPLC labeling reagent
  • Density: 1.6±0.1 g/cm3
  • Boiling Point: 467.5±55.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 236.5±31.5 °C
  • CAS Number: 211676-91-4
  • MF: C12H27NO2
  • MW: 217.34800
  • Catalog:Ion pair reagent for HPLC
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A
  • CAS Number: 1287-16-7
  • MF: C12H12FeO2
  • MW: 244.068
  • Catalog:HPLC labeling reagent
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: 158-160ºC
  • Flash Point: N/A
  • CAS Number: 78-78-4
  • MF: C5H12
  • MW: 72.14880
  • Catalog:Quinoline compound
  • Density: 0.62 g/mL at 25 °C(lit.)
  • Boiling Point: 30 °C(lit.)
  • Melting Point: -160 °C
  • Flash Point: −51 °C
  • CAS Number: 8032-32-4
  • MF: C6H6
  • MW: 78.112
  • Catalog:HPLC solvent
  • Density: 0.9±0.1 g/cm3
  • Boiling Point: 78.8±7.0 °C at 760 mmHg
  • Melting Point: -40ºC
  • Flash Point: -11.1±0.0 °C