5-Nitroindole
5-Nitroindole structure
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Common Name | 5-Nitroindole | ||
|---|---|---|---|---|
| CAS Number | 6146-52-7 | Molecular Weight | 162.15 | |
| Density | 1.4±0.1 g/cm3 | Boiling Point | 362.6±15.0 °C at 760 mmHg | |
| Molecular Formula | C8H6N2O2 | Melting Point | 140-142 °C(lit.) | |
| MSDS | Chinese USA | Flash Point | 173.1±20.4 °C | |
| Symbol |
GHS05, GHS07 |
Signal Word | Danger | |
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Generic expansion of the substrate spectrum of a DNA polymerase by directed evolution.
Nat. Biotechnol. 22(6) , 755-9, (2004) DNA polymerases recognize their substrates with exceptionally high specificity, restricting the use of unnatural nucleotides and the applications they enable. We describe a strategy to expand the substrate range of polymerases. By selecting for the extension ... |
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Hybridization properties of long nucleic acid probes for detection of variable target sequences, and development of a hybridization prediction algorithm.
Nucleic Acids Res. 38(21) , e195, (2010) One of the main problems in nucleic acid-based techniques for detection of infectious agents, such as influenza viruses, is that of nucleic acid sequence variation. DNA probes, 70-nt long, some including the nucleotide analog deoxyribose-Inosine (dInosine), w... |
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Use of 5-nitroindole-2'-deoxyribose-5'-triphosphate for labelling and detection of oligonucleotides.
Nucleosides Nucleotides 17(1-3) , 555-64, (1998) The 5'-triphosphate of 5-nitroindole-2'-deoxyriboside has been shown to be a good substrate for terminal deoxynucleotidyl transferase (TdT). An antibody has been prepared for the detection of 5-nitroindole and has been used for the detection of 5-nitroindole ... |
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Replication of a universal nucleobase provides unique insight into the role of entropy during DNA polymerization and pyrophosphorolysis.
Biochemistry 49(14) , 3009-23, (2010) Most models accounting for the efficiency and fidelity of DNA polymerization invoke the use of either hydrogen bonding contacts or complementarity of shape and size between the formed base pair. This report evaluates these mechanisms by quantifying the abilit... |
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Stabilizating effect of 5-nitroindole (universal base) on DNA duplexes immobilized on gel matrix.
J. Biomol. Struct. Dyn. 15(3) , 597-603, (1997) Effect of attachment of 1-(2-deoxy-beta-D-ribofuranosyl)-5-nitroindole (NIDR) to the ends of target sequence of oligonucleotides immobilized on gel micromatrix on stability of duplex formed by hybridization with DNA fragment was studied. It was shown that adj... |
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5-Nitroindole as an universal base analogue.
Nucleic Acids Res. 22(20) , 4039-43, (1994) 4-, 5- and 6-Nitroindole have been investigated and compared with 3-nitropyrrole as universal bases in oligodeoxynucleotides. Of these the 5-nitroindole derivative was found to be superior giving higher duplex stability, and behaving indiscriminately towards ... |
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3-Nitropyrrole and 5-nitroindole as universal bases in primers for DNA sequencing and PCR.
Nucleic Acids Res. 23(13) , 2361-6, (1995) 3-Nitropyrrole and 5-nitroindole have been assessed as universal bases in primers for dideoxy DNA sequencing and in the polymerase chain reaction (PCR). In contrast to a previous report, we have found that the introduction of more than one 3-nitropyrrole resi... |
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Hybridization properties and enzymatic replication of oligonucleotides containing the photocleavable 7-nitroindole base analog.
Nucleic Acids Res. 33(5) , 1532-43, (2005) Universal DNA base analogs having photocleavable properties would be of great interest for development of new nucleic acid fragmentation tools. The photocleavable 7-nitroindole 2'-deoxyribonucleoside d(7-Ni) was previously shown to furnish a highly efficient ... |
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Evolving a polymerase for hydrophobic base analogues.
J. Am. Chem. Soc. 131(41) , 14827-37, (2009) Hydrophobic base analogues (HBAs) have shown great promise for the expansion of the chemical and coding potential of nucleic acids but are generally poor polymerase substrates. While extensive synthetic efforts have yielded examples of HBAs with favorable sub... |
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Cationic nucleoside lipids derived from universal bases: A rational approach for siRNA transfection.
Bioconjug. Chem. 21(6) , 1062-9, (2010) Cationic nucleoside lipids (CNLs) derived from 5-nitroindole and 4-nitroimidazole bases were prepared from d-ribose by using a straightforward chemical synthesis. TEM experiments indicate that these amphiphilic molecules self-assemble to form supramolecular o... |