Journal of Separation Science 2015-08-01

Magnetic molecularly imprinted polymer for the efficient and selective preconcentration of diazinon before its determination by high-performance liquid chromatography.

Fahimeh Zare, Mehrorang Ghaedi, Ali Daneshfar, Abbas Ostovan

Index: J. Sep. Sci. 38 , 2797-803, (2015)

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Abstract

A molecularly imprinted polymer was selectively applied for solid-phase extraction and diazinon residues enrichment before high-performance liquid chromatography. Diazinon was thermally copolymerized with Fe3 O4 @polyethyleneglycol nanoparticles, methacrylic acid (functional monomer), 2-hydroxyethyl methacrylate (co-monomer), and ethylene glycol dimethacrylate (cross-linking monomer) in the presence of acetonitrile (porogen) and 2,2-azobisisobutyronitrile (initiator). Then, the imprinted diazinon was reproducibly eluted with methanol/acetic acid (9:1, v/v). The sorbent particles were characterized by X-ray diffraction, Fourier transform infrared spectroscopy, and scanning electron microscopy. The comprehensive study of variables through experimental design showed that the maximum performance was achieved under these conditions: pH 7, 10 mL sample volume, 15 mg sorbent, 10 min vortex time, 5 min ultrasonic time, 200 μL methanol/acetic acid (9:1, v/v) as eluent, and 5 min desorption time. Under optimized conditions, the molecularly imprinted polymer solid-phase extraction method demonstrated a linear range (0.02-5 g/mL), a correlation coefficient of 0.997, and 0.005 g/mL detection limit. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.