2-Methoxy-2,4-diphenyl-3(2H)-furanone
2-Methoxy-2,4-diphenyl-3(2H)-furanone structure
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Common Name | 2-Methoxy-2,4-diphenyl-3(2H)-furanone | ||
|---|---|---|---|---|
| CAS Number | 50632-57-0 | Molecular Weight | 266.291 | |
| Density | 1.2±0.1 g/cm3 | Boiling Point | 426.6±45.0 °C at 760 mmHg | |
| Molecular Formula | C17H14O3 | Melting Point | 96ºC | |
| MSDS | Chinese USA | Flash Point | 203.6±15.1 °C | |
| Symbol |
GHS07 |
Signal Word | Warning | |
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Proteolytic activity and cytokine up-regulation by non-albicans Candida albicans.
Arch. Microbiol. 197(4) , 533-7, (2015) Mouth is an important source of infections and oral infections such as Candida infections increase the risk of mortality. Our purpose was to investigate differences in proteolytic activity of non-albicans Candida albicans (non-albicans Candida) between clinic... |
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Fluorescent labeling of proteins with nile red and 2-methoxy-2,4-diphenyl-3(2H)-furanone: physicochemical basis and application to the rapid staining of sodium dodecyl sulfate polyacrylamide gels and Western blots.
Electrophoresis 22(5) , 874-80, (2001) The fluorescent hydrophobic dye Nile red allows the rapid, sensitive, and general staining of proteins in sodium dodecyl sulfate (SDS)-polyacrylamide gels. Nile red staining does not preclude further electroblotting of protein bands onto polyvinylidene difluo... |
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Rapid imaging, using a cooled charge-coupled-device, of fluorescent two-dimensional polyacrylamide gels produced by labelling proteins in the first-dimensional isoelectric focusing gel with the fluorophore 2-methoxy-2,4-diphenyl-3(2H)furanone.
Electrophoresis 9(7) , 330-9, (1988) A new method for visualising proteins in two-dimensional polyacrylamide gels was developed. Proteins were labelled with the fluorophore 2-methoxy-2,4-diphenyl-3(2H)furanone (MDPF) while present in the first-dimensional gel after isoelectric focusing and subse... |
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Endocytosis and exocytosis of protein in capillary endothelium.
J. Cell Physiol. 120(2) , 157-62, (1984) The transport of proteins across continuous capillary endothelium is believed to be mediated by micropinocytic vesicles which shuttle molecules between the lumenal and abluminal plasma membrane. We have studied the ability of capillary endothelial cells isola... |
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A fluorescent screening assay for collagenase using collagen labeled with 2-methoxy-2,4-diphenyl-3(2H)-furanone.
Anal. Biochem. 140(2) , 490-4, (1984) This report describes the use of the compound 2-methoxy-2,4-diphenyl-3(2H)-furanone to label collagen as a substrate for the detection of mammalian collagenase in a fluorescent assay which is suitable for screening large numbers of samples. The compound 2-met... |
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Fluorescence of eosinophil leukocyte granules induced by the fluorogenic reagent 2-methoxy-2,4-diphenyl-3 (2H)-furanone.
Blood Cells 19(2) , 423-30; discussion 431-3, (1993) The fluorogenic reagent MDPF forms highly fluorescent products specifically with primary amino groups (e.g., from lysine). Although widely used in analytical biochemistry, MDPF has infrequently been employed in cytochemical techniques for proteins. In this wo... |
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Simultaneous trichromatic fluorescence detection of proteins on Western blots using an amine-reactive dye in combination with alkaline phosphatase- and horseradish peroxidase-antibody conjugates.
Proteomics 3(7) , 1215-27, (2003) Three-color fluorescence detection methods are described based upon covalently coupling the dye 2-methoxy-2,4-diphenyl-2(2H)-furanone (MDPF) to proteins immobilized on poly(vinylidene difluoride) (PVDF) membranes, followed by detection of target proteins usin... |
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A multiple high-resolution mini two-dimensional polyacrylamide gel electrophoresis system: imaging two-dimensional gels using a cooled charge-coupled device after staining with silver or labeling with fluorophore.
Anal. Biochem. 195(1) , 30-7, (1991) A multiple mini two-dimensional electrophoretic method which results in three two-dimensional protein spot patterns being positioned side by side in an individual gel has been developed. Preparation time has been minimized by employing disposable capillary tu... |
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A new assay for proteases using fluorescent labeling of proteins.
Anal. Biochem. 121(2) , 290-4, (1982)
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Fluorescent monitoring of proteins during sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting.
Anal. Biochem. 144 , 537, (1985) Fluorescent labeling of proteins was found to be a very sensitive and reliable alternative to conventional methods for monitoring proteins on Western blots. Proteins were labeled with 2-methoxy-2,4-diphenyl-3(2H)-furanone (MDPF) before SDS-PAGE. After electro... |