lowicryl k4m reagent kit for

lowicryl k4m reagent kit for Structure
lowicryl k4m reagent kit for structure
Common Name lowicryl k4m reagent kit for
CAS Number 84137-04-2 Molecular Weight N/A
Density N/A Boiling Point N/A
Molecular Formula N/A Melting Point N/A
MSDS Chinese USA Flash Point 90 °C
Symbol GHS05 GHS06
GHS05, GHS06
Signal Word Danger

Localization by immunogold labelling of HIV-1 structural proteins on Lowicryl embedded HIV-1 infected cell ultrathin sections.

J. Submicrosc. Cytol. Pathol. 24(2) , 163-7, (1992)

Using several HIV-1-specific antibodies and the immunogold labelling technique, we have detected and localized distinct viral proteins on ultrathin sections of HIV-1 infected cells embedded in the Lowicryl K4M resin. Monoclonal antibodies (MAbs) against p24, ...

Histochemical study on rat tear film and ocular surface epithelial cells.

Curr. Eye Res. 17(6) , 642-9, (1998)

To investigate the histochemistry of the tear film, conjunctival goblet cells and corneal superficial epithelial cells by an in vivo cryofixation with the freeze-substitution method.Following rapid freezing of the eyeballs by an isopentane-propane mixture coo...

Localization of adenovirus DNA by in situ hybridization electron microscopy.

Microsc. Res. Tech. 21(1) , 23-31, (1992)

Biotinylated deoxyadenosine triphosphate (dATP) (Bio-7-dATP) and 3H deoxythymidine triphosphate (dTTP) labeled adenovirus DNA were hybridized in situ to thin sections of Lowicryl K4M-embedded and whole-mount extracted HeLa cells infected with adenovirus. The ...

Immunogold labeling of peroxidase in salivary gland acinar cells using an improved tissue preparation procedure: rapid freezing and Lowicryl embedding.

Eur. J. Morphol. 36 Suppl , 235-9, (1998)

The immunocytochemical localization of peroxidase in the acinar cells of the Mongolian gerbil parotid gland was studied using aldehyde-fixed tissue prepared by rapid freezing and low-temperature embedding in Lowicryl K4M resin. This freeze-embedding method wa...

Reappraisal of potassium permanganate oxidation applied to Lowicryl K4M embedded tissues processed by high pressure freezing/freeze substitution, with special reference to differential staining of the zymogen granules of rat gastric chief cells.

Arch. Histol. Cytol. 62(5) , 447-58, (1999)

The high pressure freezing/freeze substitution technique is known to yield a deep vitreous freezing of tissues. Combination of this technique with Lowicryl K4M embedding allows us histochemical studies of dynamic cellular processes with improved structural pr...

A simple contrast enhancement by potassium permanganate oxidation for Lowicryl K4M ultrathin sections prepared by high pressure freezing/freeze substitution.

J. Microsc. 201(Pt 1) , 77-83, (2001)

A simple contrast enhancement method is presented for Lowicryl K4M ultrathin sections prepared by high pressure freezing/freeze substitution. The sections were treated with an acidified potassium permanganate oxidizing solution followed by uranyl acetate and ...

Histochemical and immunohistochemical protocols for routine biopsies embedded in Lowicryl resin.

Biotech. Histochem. 78(1) , 35-42, (2003)

Tissue processing and analysis require good preservation of both the shape and content of cells. Lowicryl resin is one of the few embedding media that allow good preservation of both tissue architecture and cellular contents. Therefore, different histochemica...

Antigen retrieval trial for post-embedding immunoelectron microscopy by heating with several unmasking solutions.

J. Histochem. Cytochem. 51(8) , 989-94, (2003)

A novel antigen retrieval procedure was carried out in the post-embedding immunogold electron microscopy method to improve the stainability of the samples. This was done by weakly fixing cultured Helicobacter pylori (ATCC43504) and embedding in Lowicryl K4M. ...

Anionic sites on Reissner's membrane, stria vascularis, and spiral prominence.

J. Histochem. Cytochem. 43(3) , 299-305, (1995)

We demonstrated anionic sites on the lateral wall of cochlear duct and Reissner's membrane (RM) of ICR mice by Lowicryl K4M resin post-embedding and poly-L-lysine-colloidal gold conjugate (PL-CG) as a polycationic probe. The basement membrane and endolymphati...

Contrast-enhanced immunoelectron microscopy for Helicobacter pylori.

J. Microbiol. Methods 42(2) , 121-7, (2000)

Since a method of contrast enhancement for immunoelectron microscopy has not been available in bacteriology, the morphological localization of proteins of Helicobacter pylori is not well known. In this report, we established a method of contrast enhancement i...