Journal of Microbiological Methods 2000-10-01

Contrast-enhanced immunoelectron microscopy for Helicobacter pylori.

W Hong, S Morimatsu, T Goto, G Sachs, D R Scott, D L Weeks, T Kohno, C Morita, T Nakano, Y Fujioka, K Sano

Index: J. Microbiol. Methods 42(2) , 121-7, (2000)

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Abstract

Since a method of contrast enhancement for immunoelectron microscopy has not been available in bacteriology, the morphological localization of proteins of Helicobacter pylori is not well known. In this report, we established a method of contrast enhancement in immunoelectron microscopy in this organism. Immunostained ultrathin sections are stained with a mixture of alcian blue and osmium tetroxide prior to staining with uranyl acetate. This method of staining provided good contrast enhancement of the bacterial cell wall and membrane without any loss of immunolabeled gold particles on the ultrathin section.