Native Pseudomonas sp. Creatinine amidohydrolase

Native Pseudomonas sp. Creatinine amidohydrolase Structure
Native Pseudomonas sp. Creatinine amidohydrolase structure
Common Name Native Pseudomonas sp. Creatinine amidohydrolase
CAS Number 9025-13-2 Molecular Weight 188.146
Density 1.2±0.1 g/cm3 Boiling Point 194.5±35.0 °C at 760 mmHg
Molecular Formula C9H7F3O Melting Point N/A
MSDS Chinese USA Flash Point 78.1±17.4 °C

Analytical expression of non-steady-state concentrations and current pertaining to compounds present in the enzyme membrane of biosensor.

J. Phys. Chem. A 115(17) , 4299-306, (2011)

A mathematical model of trienzyme biosensor at an internal diffusion limitation for a non-steady-state condition has been developed. The model is based on diffusion equations containing a linear term related to Michaelis-Menten kinetics of the enzymatic react...

Immobilization of creatininase, creatinase and sarcosine oxidase on iron oxide nanoparticles/chitosan-g-polyaniline modified Pt electrode for detection of creatinine.

Enzyme Microb. Technol. 50(4-5) , 247-54, (2012)

Commercial enzymes, creatininase (CA) from Pseudomonas sp, creatinase (CI) from Pseudomonas sp, sarcosine oxidase (SO) from Bacillus sp were co-immobilized onto iron oxide nanoparticles/chitosan-graft-polyaniline (Fe(3)O(4)-NPs/CHIT-g-PANI) composite film ele...

Amperometric creatinine biosensor based on covalently coimmobilized enzymes onto carboxylated multiwalled carbon nanotubes/polyaniline composite film.

Anal. Biochem. 419(2) , 277-83, (2011)

A mixture of commercial creatinine amidohydrolase (CA), creatine amidinohydrolase (CI), and sarcosine oxidase (SO) was coimmobilized covalently via N-ethyl-N'-(3-dimethylaminopropyl) carbodiimide (EDC) and N-hydroxy succinimide (NHS) chemistry onto carboxylat...

One-chip biosensor for simultaneous disease marker/calibration substance measurement in human urine by electrochemical surface plasmon resonance method.

Biosens. Bioelectron. 26(4) , 1536-42, (2010)

We have developed a miniaturized electrochemical surface plasmon resonance biosensor for measuring two biomolecules that have very different molecular sizes, one is transferrin (MW=75 kDa) as a disease marker protein, the other is creatinine (MW=113) as a cal...

Simultaneous detection of creatine and creatinine using a sequential injection analysis/biosensor system.

Prep Biochem Biotechnol. 36(4) , 287-96, (2006)

Carbon paste based biosensors for the determination of creatine and creatinine have been integrated into a sequential injection system. Applying the multi-enzyme sequence of creatininase (CA), and/or creatinase (CI) and sarcosine oxidase (SO), hydrogen peroxi...

Plasma creatinine determination in mice and rats: an enzymatic method compares favorably with a high-performance liquid chromatography assay.

Kidney Int. 71(1) , 74-8, (2007)

The use of the colorimetric Jaffé method for the measurement of creatinine in mouse and rat plasma has been criticized as prior studies have shown a dramatic overestimation. We compared a colorimetric picric acid, an enzymatic, and a high-performance liquid c...

A stable three-enzyme creatinine biosensor. 3. Immobilization of creatinine amidohydrolase and sensor development.

Acta Biomater. 1(2) , 193-9, (2005)

We describe the development of an extended use amperometric three-enzyme creatinine biosensor and the successful chemical modification and immobilization of the enzyme creatinine amidohydrolase using polyurethane prepolymers. Creatinine amidohydrolase is sign...

Effect of chronic kidney disease and comorbid conditions on health care costs: A 10-year observational study in a general population.

Am. J. Nephrol. 31(3) , 222-9, (2010)

Chronic kidney disease (CKD) is common, but the longitudinal effects of CKD and associated comorbidities on health care costs in the general population are unknown.Population-based cohort study of 2,988 subjects in Germany, aged 25-74 years at baseline, who p...

Influence of metal cofactors and water on the catalytic mechanism of creatininase-creatinine in aqueous solution from molecular dynamics simulation and quantum study.

J. Comput. Aided Mol. Des. 24(10) , 879-86, (2010)

The reaction mechanism of creatinine-creatininase binding to form creatine as a final product has been investigated by using a combined ab initio quantum mechanical/molecular mechanical approach and classical molecular dynamics (MD) simulations. In MD simulat...

Polycystic kidneys have decreased vascular density: a micro-CT study.

Microcirculation 20(2) , 183-9, (2013)

Polycystic kidney disease (PKD) is a common cause of end-stage renal failure and many of these patients suffer vascular dysfunction and hypertension. It remains unclear whether PKD is associated with abnormal microvascular structure. Thus, this study examined...