![]() Native Pseudomonas sp. Creatinine amidohydrolase structure
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Common Name | Native Pseudomonas sp. Creatinine amidohydrolase | ||
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CAS Number | 9025-13-2 | Molecular Weight | 188.146 | |
Density | 1.2±0.1 g/cm3 | Boiling Point | 194.5±35.0 °C at 760 mmHg | |
Molecular Formula | C9H7F3O | Melting Point | N/A | |
MSDS | Chinese USA | Flash Point | 78.1±17.4 °C |
Analytical expression of non-steady-state concentrations and current pertaining to compounds present in the enzyme membrane of biosensor.
J. Phys. Chem. A 115(17) , 4299-306, (2011) A mathematical model of trienzyme biosensor at an internal diffusion limitation for a non-steady-state condition has been developed. The model is based on diffusion equations containing a linear term related to Michaelis-Menten kinetics of the enzymatic react... |
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Immobilization of creatininase, creatinase and sarcosine oxidase on iron oxide nanoparticles/chitosan-g-polyaniline modified Pt electrode for detection of creatinine.
Enzyme Microb. Technol. 50(4-5) , 247-54, (2012) Commercial enzymes, creatininase (CA) from Pseudomonas sp, creatinase (CI) from Pseudomonas sp, sarcosine oxidase (SO) from Bacillus sp were co-immobilized onto iron oxide nanoparticles/chitosan-graft-polyaniline (Fe(3)O(4)-NPs/CHIT-g-PANI) composite film ele... |
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Amperometric creatinine biosensor based on covalently coimmobilized enzymes onto carboxylated multiwalled carbon nanotubes/polyaniline composite film.
Anal. Biochem. 419(2) , 277-83, (2011) A mixture of commercial creatinine amidohydrolase (CA), creatine amidinohydrolase (CI), and sarcosine oxidase (SO) was coimmobilized covalently via N-ethyl-N'-(3-dimethylaminopropyl) carbodiimide (EDC) and N-hydroxy succinimide (NHS) chemistry onto carboxylat... |
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One-chip biosensor for simultaneous disease marker/calibration substance measurement in human urine by electrochemical surface plasmon resonance method.
Biosens. Bioelectron. 26(4) , 1536-42, (2010) We have developed a miniaturized electrochemical surface plasmon resonance biosensor for measuring two biomolecules that have very different molecular sizes, one is transferrin (MW=75 kDa) as a disease marker protein, the other is creatinine (MW=113) as a cal... |
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Simultaneous detection of creatine and creatinine using a sequential injection analysis/biosensor system.
Prep Biochem Biotechnol. 36(4) , 287-96, (2006) Carbon paste based biosensors for the determination of creatine and creatinine have been integrated into a sequential injection system. Applying the multi-enzyme sequence of creatininase (CA), and/or creatinase (CI) and sarcosine oxidase (SO), hydrogen peroxi... |
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Plasma creatinine determination in mice and rats: an enzymatic method compares favorably with a high-performance liquid chromatography assay.
Kidney Int. 71(1) , 74-8, (2007) The use of the colorimetric Jaffé method for the measurement of creatinine in mouse and rat plasma has been criticized as prior studies have shown a dramatic overestimation. We compared a colorimetric picric acid, an enzymatic, and a high-performance liquid c... |
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A stable three-enzyme creatinine biosensor. 3. Immobilization of creatinine amidohydrolase and sensor development.
Acta Biomater. 1(2) , 193-9, (2005) We describe the development of an extended use amperometric three-enzyme creatinine biosensor and the successful chemical modification and immobilization of the enzyme creatinine amidohydrolase using polyurethane prepolymers. Creatinine amidohydrolase is sign... |
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Effect of chronic kidney disease and comorbid conditions on health care costs: A 10-year observational study in a general population.
Am. J. Nephrol. 31(3) , 222-9, (2010) Chronic kidney disease (CKD) is common, but the longitudinal effects of CKD and associated comorbidities on health care costs in the general population are unknown.Population-based cohort study of 2,988 subjects in Germany, aged 25-74 years at baseline, who p... |
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Influence of metal cofactors and water on the catalytic mechanism of creatininase-creatinine in aqueous solution from molecular dynamics simulation and quantum study.
J. Comput. Aided Mol. Des. 24(10) , 879-86, (2010) The reaction mechanism of creatinine-creatininase binding to form creatine as a final product has been investigated by using a combined ab initio quantum mechanical/molecular mechanical approach and classical molecular dynamics (MD) simulations. In MD simulat... |
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Polycystic kidneys have decreased vascular density: a micro-CT study.
Microcirculation 20(2) , 183-9, (2013) Polycystic kidney disease (PKD) is a common cause of end-stage renal failure and many of these patients suffer vascular dysfunction and hypertension. It remains unclear whether PKD is associated with abnormal microvascular structure. Thus, this study examined... |