Preparative Biochemistry and Biotechnology 2006-01-01

Simultaneous detection of creatine and creatinine using a sequential injection analysis/biosensor system.

Raluca-Ioana Stefan-van Staden, Rahel Girmai Bokretsion, Jacobus F van Staden, Hassan Y Aboul-Enein

Index: Prep Biochem Biotechnol. 36(4) , 287-96, (2006)

Full Text: HTML

Abstract

Carbon paste based biosensors for the determination of creatine and creatinine have been integrated into a sequential injection system. Applying the multi-enzyme sequence of creatininase (CA), and/or creatinase (CI) and sarcosine oxidase (SO), hydrogen peroxide has been detected amperometrically. The linear concentration ranges are of pmol/L to nmol/L magnitude, with very low limits of detection. The proposed SIA system can be utilized reliably for the on-line simultaneous detection of creatine and creatinine in pharmaceutical products, as well as in serum samples, with a rate of 34 samples per hour and RSD values better than 0.16% (n=10).