PNGase F
PNGase F structure
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Common Name | PNGase F | ||
|---|---|---|---|---|
| CAS Number | 83534-39-8 | Molecular Weight | N/A | |
| Density | N/A | Boiling Point | N/A | |
| Molecular Formula | N/A | Melting Point | N/A | |
| MSDS | USA | Flash Point | N/A | |
| Symbol |
GHS05, GHS06, GHS09 |
Signal Word | Danger | |
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The characterization and quantitation of glycomic changes in CHO cells during a bioreactor campaign.
Biotechnol. Bioeng. 109(12) , 3007-17, (2012) Within the biotechnology industry there is a continuous drive to better and more fully understand the biopharmaceutical process in order to achieve better process control. A method to monitor and quantitate glycomic changes that occur in CHO cells during a bi... |
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N-Terminal signal sequence is required for cellular trafficking and hyaluronan-depolymerization of KIAA1199.
FEBS Lett. 588(1) , 111-6, (2014) Recently, we disclosed that KIAA1199-mediated hyaluronan (HA) depolymerization requires an acidic cellular microenvironment (e.g. clathrin-coated vesicles or early endosomes), but no information about the structural basis underlying the cellular targeting and... |
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Liver and muscle hemojuvelin are differently glycosylated.
BMC Biochem. 12 , 52, (2011) Hemojuvelin (HJV) is one of essential components for expression of hepcidin, a hormone which regulates iron transport. HJV is mainly expressed in muscle and liver, and processing of HJV in both tissues is similar. However, hepcidin is expressed in liver but n... |
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Reversal of neuropathic pain in diabetes by targeting glycosylation of Ca(V)3.2 T-type calcium channels.
Diabetes 62(11) , 3828-38, (2013) It has been established that Ca(V)3.2 T-type voltage-gated calcium channels (T-channels) play a key role in the sensitized (hyperexcitable) state of nociceptive sensory neurons (nociceptors) in response to hyperglycemia associated with diabetes, which in turn... |
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Effect of the GPI anchor of human Thy-1 on antibody recognition and function.
Lab. Invest. 93(3) , 365-74, (2013) Thymocyte differentiation antigen-1 (Thy-1) is a glycosylphosphatidylinositol (GPI)-linked cell surface glycoprotein expressed on numerous cell types, which regulates signals affecting cell adhesion, migration, differentiation, and survival. In addition, Thy-... |
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LC-MS/MS characterization of O-glycosylation sites and glycan structures of human cerebrospinal fluid glycoproteins.
J. Proteome Res. 12(2) , 573-84, (2013) The GalNAc O-glycosylation on Ser/Thr residues of extracellular proteins has not been well characterized from a proteomics perspective. We previously reported a sialic acid capture-and-release protocol to enrich tryptic N- and O-glycopeptides from human cereb... |
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In vivo deglycosylation of recombinant proteins in plants by co-expression with bacterial PNGase F.
Bioengineered 4(5) , 338-42, (2013) At present, several eukaryotic expression systems including yeast, insect and mammalian cells and plants are used for the production of recombinant proteins. Proteins with potential N-glycosylation sites are efficiently glycosylated when expressed in these sy... |
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Avidity confers FcγR binding and immune effector function to aglycosylated immunoglobulin G1.
J. Mol. Recognit. 25(3) , 147-54, (2012) Immunoglobulin G (IgG) antibodies are an integral part of the adaptive immune response that provide a direct link between humoral and cellular components of the immune system. Insights into relationships between the structure and function of human IgGs have p... |
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Some characteristics of a new glycopeptidase acting on aspartylglycosylamine linkages.
J. Biochem. 84(6) , 1467-73, (1978) A new type of glycopeptidase hydrolyzing beta-aspartylglycosylamine linkages was partially purified from almond emulsin by chromatography on Sephadex G-200 and DE 52. The enzyme degraded stem bromelain glycopeptide, Asn-Asn(Man3,Xyl1,Fuc1,GlcNAc2)-Glu-Ser-Ser... |
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NMR characterization of the interaction between the PUB domain of peptide:N-glycanase and ubiquitin-like domain of HR23
FEBS Lett. 586(8) , 1141-6, (2012) Highlights ► PUB domain of PNGase binds to HR23–UBL more preferentially than ubiquitin chains. ► 3D model of the PUB–UBL complex is presented based on NMR data. ► The PUB–UBL complex is mediated by electrostatic interactions. ► PUB binds the UBL surface invol... |