![]() (+)生物素-N-琥珀酰亚胺基酯结构式
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常用名 | (+)生物素-N-琥珀酰亚胺基酯 | 英文名 | Biotin NHS |
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CAS号 | 35013-72-0 | 分子量 | 341.383 | |
密度 | 1.5±0.1 g/cm3 | 沸点 | N/A | |
分子式 | C14H19N3O5S | 熔点 | 212-214 °C | |
MSDS | 中文版 美版 | 闪点 | N/A |
Rapid fluorescent detection of Escherichia coli K88 based on DNA aptamer library as direct and specific reporter combined with immuno-magnetic separation.
J. Fluoresc. 24(4) , 1159-68, (2014) Nucleic acid aptamers have long demonstrated the capacity to bind cells with high affinity so that they have been utilized to diagnose various important pathogens. In this study, a DNA aptamer library was on initial efforts developed to act as a specific repo... |
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Xepac protein and IP3/Ca2+ pathway implication during Xenopus laevis vitellogenesis.
Zygote 23(1) , 99-110, (2015) The objective of this study was to elucidate the signalling pathways initiated by cAMP once inside the Xenopus laevis oocyte, where it triggers and maintains vitellogenin endocytic uptake. Our results showed the presence of Xepac transcripts at all stages of ... |
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The use of the avidin-biotin complex as a tool in molecular biology.
Methods Biochem. Anal. New York, NY 26 , 1-45, (1980)
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K. Hoffmann et al.
J. Am. Chem. Soc. 100 , 3585, (1978)
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On-resin biotinylation of chemically synthesized proteins for one-step purification.
Anal. Biochem. 170 , 502, (1988) A general, convenient, one-step purification procedure for chemically synthesized proteins present in low yields using on-resin biotinylation is reported. The protein, terminally deprotected and neutralized on-resin, is stirred in dimethylformamide and then b... |
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Allergenic lipid transfer proteins from plant-derived foods do not immunologically and clinically behave homogeneously: the kiwifruit LTP as a model.
PLoS ONE 6(11) , e27856, (2011) Food allergy is increasingly common worldwide. Tools for allergy diagnosis measuring IgE improved much since allergenic molecules and microarrays started to be used. IgE response toward allergens belonging to the same group of molecules has not been comprehen... |
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Serum levels, ontogeny and heritability of chicken mannan-binding lectin (MBL).
Immunology 94 , 587-593, (1994) Mannan-binding lectin (MBL) is a serum lectin found in mammals and recently also in birds. It is thought to play an important role in the innate immune defence through binding to surface carbohydrates on micro-organisms followed by complement activation via t... |
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Nonregeneration protocol for surface plasmon resonance: study of high-affinity interaction with high-density biosensors.
Anal. Chem. 78 , 1841-1848, (2008) Surface plasmon resonance (SPR) has been used in determining kinetics and thermodynamics of biological interaction in the past decades. One difficulty encountered in this technology is the need for a proper regeneration, which means the removal of analytes fr... |
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Synthesis of adenosine derivatives as transcription initiators and preparation of 5' fluorescein- and biotin-labeled RNA through one-step in vitro transcription.
RNA 9 , 1562-1570, (2003) Expanding our previous finding of an adenosine-initiated transcription system, we now demonstrate that either the 5' site or the N6 site of adenosine nucleotides can be modified extensively without abolishing their ability to initiate transcription under the ... |
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Homogeneous assays for riboflavin mediated by the interaction between enzyme-biotin and avidin-riboflavin conjugates.
Anal. Biochem. 231 , 400-406, (1995) Homogeneous-type enzyme-linked competitive binding assays utilizing the synthetic enzyme-biotin and avidin-riboflavin conjugates are developed for the detection of riboflavin as well as its binder protein. The activity of the enzyme-biotin conjugate is inhibi... |