5-碘乙酰氨基荧光素
5-碘乙酰氨基荧光素结构式
|
常用名 | 5-碘乙酰氨基荧光素 | 英文名 | 5-(Iodoacetamido)fluorescein |
|---|---|---|---|---|
| CAS号 | 63368-54-7 | 分子量 | 515.25400 | |
| 密度 | 1.95 g/cm3 | 沸点 | 788.4ºC at 760 mmHg | |
| 分子式 | C22H14INO6 | 熔点 | 265-267ºC (dec.)(lit.) | |
| MSDS | 中文版 美版 | 闪点 | 430.6ºC |
|
Combination of direct infusion mass spectrometry and gas chromatography mass spectrometry for toxicometabolomic study of red blood cells and serum of mice Mus musculus after mercury exposure.
J. Chromatogr. B. Analyt. Technol. Biomed. Life Sci. 985 , 75-84, (2015) Although mercury (Hg) is an important environmental and occupational pollutant, its toxicological effects, especially in serum and red blood cells (RBCs), have been scarcely studied. A toxicometabolomics workflow based on high resolution mass spectrometry app... |
|
|
Optical control of protein-protein interactions via blue light-induced domain swapping.
Biochemistry 53(30) , 5008-16, (2014) The design of new optogenetic tools for controlling protein function would be facilitated by the development of protein scaffolds that undergo large, well-defined structural changes upon exposure to light. Domain swapping, a process in which a structural elem... |
|
|
Redox signalling to nuclear regulatory proteins by reactive oxygen species contributes to oestrogen-induced growth of breast cancer cells.
Br. J. Cancer 112 , 1687-702, (2015) 17β-Oestradiol (E2)-induced reactive oxygen species (ROS) have been implicated in regulating the growth of breast cancer cells. However, the underlying mechanism of this is not clear. Here we show how ROS through a novel redox signalling pathway involving nuc... |
|
|
Analysis of alpha-1-acid glycoprotein isoforms using CE-LIF with fluorescent thiol derivatization.
Electrophoresis 33(7) , 1113-9, (2012) The analysis of glycoprotein isoforms is of high interest in the biomedical field and clinical chemistry. Many studies have demonstrated that some glycoprotein isoforms could serve as biomarkers for several major diseases, such as cancers and vascular disease... |
|
|
Impact of glutathione on the allergenicity of the peach lipid transfer protein Pru p 3.
J. Investig. Allergol. Clin. Immunol. 25(1) , 47-54, (2015) The allergenic potential of proteins can be altered under various physicochemical conditions. Glutathione (GSH) is a reducing agent that is used as an antioxidant in food products. We aimed to characterize the natural folding of peach proteins and test the al... |
|
|
Repair of peroxynitrite damage to tubulin by the thioredoxin reductase system.
Free Radic. Biol. Med. 36(4) , 497-506, (2004) Cumulative oxidative damage to proteins coupled with a decrease in repair has been implicated in the pathology of several neurodegenerative diseases. Herein we report that peroxynitrite-induced disulfides in porcine brain tubulin are repaired by the thioredox... |
|
|
Identification and localization of unpaired cysteine residues in monoclonal antibodies by fluorescence labeling and mass spectrometry.
Anal. Chem. 81(15) , 6449-57, (2009) Each human IgG1 antibody contains a total of thirty-two cysteine residues. In theory, all of them are involved in disulfide bonds, and no free sulfhydryl should be detected. However, literature has suggested that the presence of low levels of free sulfhydryl ... |
|
|
Highly sensitive simultaneous detection of cultured cellular thiols by laser induced fluorescence-capillary electrophoresis.
Electrophoresis 26(6) , 1063-70, (2005) We have recently described a new method to determine physiological thiols, in which the quantification of plasma homocysteine, cysteine, cysteinylglycine, glutathione, and glutamylcysteine was achieved after derivatization with 5-iodoacetamidofluorescein. Sam... |
|
|
Modulation of the redox state of tubulin by the glutathione/glutaredoxin reductase system.
Biochem. Biophys. Res. Commun. 314(2) , 555-60, (2004) Alterations in the redox status of proteins have been implicated in the pathology of several neurodegenerative diseases. We report that peroxynitrite-induced disulfides in porcine brain tubulin are repaired by the glutaredoxin reductase system composed of glu... |
|
|
Ascorbic acid reduction of microtubule protein disulfides and its relevance to protein S-nitrosylation assays.
Biochem. Biophys. Res. Commun. 340(2) , 347-52, (2006) The biotin switch assay was developed to aid in the identification of S-nitrosylated proteins in different cell types. However, our work with microtubule proteins including tubulin and its associated proteins tau and microtubule-associated protein-2 shows tha... |