考马斯亮蓝R250
考马斯亮蓝R250结构式
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常用名 | 考马斯亮蓝R250 | 英文名 | Brilliant Blue R250 |
|---|---|---|---|---|
| CAS号 | 6104-59-2 | 分子量 | 825.966 | |
| 密度 | N/A | 沸点 | N/A | |
| 分子式 | C45H44N3NaO7S2 | 熔点 | 174-180 °C | |
| MSDS | 美版 | 闪点 | 14 °C | |
| 符号 |
GHS02, GHS07 |
信号词 | Warning |
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Titer on chip: new analytical tool for influenza vaccine potency determination.
PLoS ONE 9(10) , e109616, (2014) Titer on Chip (Flu-ToC) is a new technique for quantification of influenza hemagglutinin (HA) concentration. In order to evaluate the potential of this new technique, a comparison of Flu-ToC to more conventional methods was conducted using recombinant HA prod... |
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Rapid detection of proteins in polyacrylamide electrophoresis gels with Direct Red 81 and Amido Black.
Methods Mol. Biol. 869 , 585-9, (2012) Proteins separated by SDS-polyacrylamide gel electrophoresis need to be stained with organic dyes to be visualized and to enable comparisons to be made between the intensity of protein bands to observe and determine differences in protein concentration. The s... |
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Development of an affordable dye-stained microalbuminuria screening test.
Nephrol. Dial. Transplant. 24(5) , 1485-90, (2009) A simple spot test was developed, which allows quantification of microalbuminuria. Evaluation was carried out according to the ISO 15189 guidelines.Urine was spotted on cellulose acetate strips and stained using different sensitive protein binding dyes (nigro... |
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Reconfiguring the quorum-sensing regulator SdiA of Escherichia coli to control biofilm formation via indole and N-acylhomoserine lactones.
Appl. Environ. Microbiol. 75 , 1703-16, (2009) SdiA is a homolog of quorum-sensing regulators that detects N-acylhomoserine lactone (AHL) signals from other bacteria. Escherichia coli uses SdiA to reduce its biofilm formation in the presence of both AHLs and its own signal indole. Here we reconfigured Sdi... |
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Lipopolysaccharide inhibits the channel activity of the P2X7 receptor.
Mediators Inflamm. 2011 , 152625, (2011) The purinergic P2X7 receptor (P2X7R) plays an important role during the immune response, participating in several events such as cytokine release, apoptosis, and necrosis. The bacterial endotoxin lipopolysaccharide (LPS) is one of the strongest stimuli of the... |
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TEMED-enhanced photoluminescent imaging of human serum proteins by quantum dots after PAGE.
Methods Mol. Biol. 869 , 511-20, (2012) Polyacrylamide gel electrophoresis (PAGE) has been one of the most powerful and widely used separation techniques for complex biological samples, whose traditional detection methods include organic dye or silver staining. As a simple, convenient, and ultrasen... |
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In vitro secretion and activity profiles of matrix metalloproteinases, MMP-9 and MMP-2, in human term extra-placental membranes after exposure to Escherichia coli.
Reprod. Biol. Endocrinol. 9 , 13, (2011) Premature rupture of fetal membranes (PROM) complicated with intrauterine infection has been associated to alterations of the extracellular matrix (ECM) homeostasis. The aim of this work was to evaluate the integral/functional response of the amnion (AMN) and... |
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Dystrophin proteolysis: a potential target for MMP-2 and its prevention by ischemic preconditioning.
Am. J. Physiol. Heart Circ. Physiol. 307(1) , H88-96, (2014) Dystrophin is responsible for the mechanical stabilization of the sarcolemma, and it has been shown that it is one of the most sensitive proteins to ischemic injury. However, the enzyme responsible for this proteolysis is still unknown. Isolated rabbit hearts... |
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Fast and sensitive coomassie staining in quantitative proteomics.
Methods Mol. Biol. 893 , 47-59, (2012) Proteins separated by two-dimensional gel electrophoresis can be visualized by in-gel detection using -different staining methods. Ideally, the dye should bind non-covalently to the protein following a linear response curve. Since protein concentrations in bi... |
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Quantitation of protein on gels and blots by infrared fluorescence of Coomassie blue and Fast Green.
Anal. Biochem. 350(2) , 233-8, (2006) Coomassie blue staining of gels and blots is commonly employed for detection and quantitation of proteins by densitometry. We found that Coomassie blue or Fast Green FCF bound to protein fluoresces in the near infrared. We took advantage of this property to d... |