青霉素酰胺酶 来源于大肠杆菌
青霉素酰胺酶 来源于大肠杆菌结构式
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常用名 | 青霉素酰胺酶 来源于大肠杆菌 | 英文名 | Penicillin amidase |
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| CAS号 | 9014-06-6 | 分子量 | N/A | |
| 密度 | N/A | 沸点 | N/A | |
| 分子式 | N/A | 熔点 | N/A | |
| MSDS | 美版 | 闪点 | N/A |
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Structure of a class III engineered cephalosporin acylase: comparisons with class I acylase and implications for differences in substrate specificity and catalytic activity.
Biochem. J. 451(2) , 217-26, (2013) The crystal structure of the wild-type form of glutaryl-7-ACA (7-aminocephalosporanic acid) acylase from Pseudomonas N176 and a double mutant of the protein (H57βS/H70βS) that displays enhanced catalytic efficiency on cephalosporin C over glutaryl-7-aminoceph... |
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Enantioselective acylation of β-phenylalanine acid and its derivatives catalyzed by penicillin G acylase from Alcaligenes faecalis.
Prep Biochem Biotechnol. 43(2) , 207-16, (2013) This study developed a simple, efficient method for producing racemic β-phenylalanine acid (BPA) and its derivatives via the enantioselective acylation catalyzed by the penicillin G acylase from Alcaligenes faecalis (Af-PGA). When the reaction was run at 25°C... |
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Engineering the substrate specificity of a thermophilic penicillin acylase from thermus thermophilus.
Appl. Environ. Microbiol. 79(5) , 1555-62, (2013) A homologue of the Escherichia coli penicillin acylase is encoded in the genomes of several thermophiles, including in different Thermus thermophilus strains. Although the natural substrate of this enzyme is not known, this acylase shows a marked preference f... |
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Polymer nanoreactors shown to produce and release antibiotics locally.
Chem. Commun. (Camb.) 49(2) , 128-30, (2013) We designed and prepared nanoreactors based on a poly(2-methyloxazoline)-block-poly(dimethylsiloxane)-block-poly(2-methyloxazoline (PMOXA-b-PDMS-b-PMOXA) amphiphilic triblock copolymer encapsulating the enzyme penicillin acylase for local and controlled produ... |
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Functional analysis of four bile salt hydrolase and penicillin acylase family members in Lactobacillus plantarum WCFS1.
Appl. Environ. Microbiol. 74(15) , 4719-26, (2008) Bile salts play an important role in the digestion of lipids in vertebrates and are synthesized and conjugated to either glycine or taurine in the liver. Following secretion of bile salts into the small intestine, intestinal microbes are capable of deconjugat... |
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Improving the diastereoselectivity of penicillin G acylase for ampicillin synthesis from racemic substrates.
Protein Eng. Des. Sel. 25(3) , 135-44, (2012) Semi-synthetic β-lactam antibiotics are synthesized enzymatically with the use of penicillin G acylase (PGA). Currently, PGA only exhibits weak diastereoselectivity with respect to the alpha amino group of rac-phenylglycine methyl ester (rac-PGME) when it is ... |
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The N-terminal nucleophile serine of cephalosporin acylase executes the second autoproteolytic cleavage and acylpeptide hydrolysis.
J. Biol. Chem. 286(27) , 24476-86, (2011) Cephalosporin acylase (CA) precursor is translated as a single polypeptide chain and folds into a self-activating pre-protein. Activation requires two peptide bond cleavages that excise an internal spacer to form the mature αβ heterodimer. Using Q-TOF LC-MS, ... |
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Enhancement of activity of cross-linked enzyme aggregates by a sugar-assisted precipitation strategy: technical development and molecular mechanism.
J. Biotechnol. 156(1) , 30-8, (2011) The precipitation of enzyme causes the major activity loss in the conventional protocol for CLEAs preparation. Herein, a sugar-assisted strategy was developed to minimize the activity loss in the step of enzyme precipitation by adding sugar as the stabilizer,... |
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Development of a new enzyme-responsive self-immolative spacer conjugate applicable to the controlled drug release
Bioorg. Med. Chem. 20(11) , 3465-9, (2012) A new self-immolative spacer conjugate based on a chemical adaptor unit aiming at controlled releasing drugs was designed and synthesized. It releases a fluorophore which was used as a model drug via a spontaneous cyclization mechanism after cleavage of an en... |
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Purification and characterization of YxeI, a penicillin acylase from Bacillus subtilis.
Int. J. Biol. Macromol. 50(1) , 25-30, (2012) The paper reports the purification and characterization of the first penicillin acylase from Bacillus subtilis. YxeI, the protein annotated as hypothetical, coded by the gene yxeI in the open reading frame between iol and hut operons in B. subtilis was cloned... |