V8蛋白酶
V8蛋白酶结构式
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常用名 | V8蛋白酶 | 英文名 | Glu-C |
|---|---|---|---|---|
| CAS号 | 66676-43-5 | 分子量 | N/A | |
| 密度 | N/A | 沸点 | N/A | |
| 分子式 | N/A | 熔点 | N/A | |
| MSDS | 中文版 美版 | 闪点 | N/A | |
| 符号 |
GHS07, GHS08 |
信号词 | Danger |
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pNovo+: de novo peptide sequencing using complementary HCD and ETD tandem mass spectra.
J. Proteome Res. 12(2) , 615-25, (2013) De novo peptide sequencing is the only tool for extracting peptide sequences directly from tandem mass spectrometry (MS) data without any protein database. However, neither the accuracy nor the efficiency of de novo sequencing has been satisfactory, mainly du... |
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Probing the 3-D structure, dynamics, and stability of bacterial collagenase collagen binding domain (apo- versus holo-) by limited proteolysis MALDI-TOF MS.
J. Am. Soc. Mass Spectrom. 23(3) , 505-19, (2012) Pairing limited proteolysis and matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) to probe clostridial collagenase collagen binding domain (CBD) reveals the solution dynamics and stability of the protein, as these fac... |
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Propeptide processing and proteolytic activity of proenzymes of the staphylococcal and enterococcal GluV8-family protease.
Indian J. Biochem. Biophys. 49(6) , 421-7, (2012) Proenzymes with various lengths of propeptides have been observed in GluV8 from Staphylococcus aureus and GluSE from S. epidermidis. However, the production mechanism of these proenzymes and roles of truncated propeptides have yet to be elucidated. Here we de... |
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Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis.
J. Biol. Chem. 252 , 1102, (1977) A rapid and convenient method for peptide mapping of proteins has been developed. The technique, which is especially suitable for analysis of proteins that have been isolated from gels containg sodium dodecyl sulfate, involves partial enzymatic proteolysis in... |
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Monoclonal antibodies against the Escherichia coli DNA repair protein RadA/Sms.
Hybridoma (Larchmt.) 31(1) , 25-31, (2012) The RadA/Sms protein facilitates DNA repair in Escherichia coli cells damaged by UV radiation, X-rays, and chemical agents. However, the precise mechanism by which RadA/Sms aids DNA repair is unknown. Here we report the production of monoclonal antibodies (MA... |
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Qualitative and quantitative analysis of glycated proteins in human plasma by glucose isotopic labeling with ¹³C6-reducing sugars.
Methods Mol. Biol. 728 , 219-32, (2011) Glucose is the predominant source of energy in cells. However, a chronic high glucose exposure of proteins modifies a number of biological pathways, known as glucotoxicity. Several studies have suggested that this impaired protein function is associated in pa... |
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Substrate specificity of glutamyl endopeptidase (GE): hydrolysis studies with a bovine α-casein preparation.
Food Chem. 136(2) , 501-12, (2013) Glutamyl endopeptidase (GE) from Alcalase™ 2.4 L was purified using hydrophobic interaction (HIC) and ion-exchange (IEX) chromatography. The yield of GE obtained was approximately 42%. Bovine α-casein (containing α(s1)- and α(s2)-casein) was digested with GE ... |
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Improve the coverage for the analysis of phosphoproteome of HeLa cells by a tandem digestion approach.
J. Proteome Res. 11(5) , 2828-37, (2012) Complete coverage of all phosphorylation sites in a proteome is the ultimate goal for large-scale phosphoproteome analysis. However, only making use of one protease trypsin for protein digestion cannot cover all phosphorylation sites, because not all tryptic ... |
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Increasing phosphoproteomic coverage through sequential digestion by complementary proteases.
Anal. Bioanal. Chem 402(2) , 711-20, (2012) Protein phosphorylation is a reversible post-translational modification known to regulate protein function, subcellular localization, complex formation, and protein degradation. Detailed phosphoproteomic information is critical to kinomic studies of signal tr... |
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Nucleotide sequence of the serine protease gene of Staphylococcus aureus, strain V8.
Nucleic Acids Res. 15(16) , 6757, (1987)
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