蔗糖磷酸化酶
蔗糖磷酸化酶结构式
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常用名 | 蔗糖磷酸化酶 | 英文名 | SUCROSE PHOSPHORYLASE |
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| CAS号 | 9074-06-0 | 分子量 | 416.416 | |
| 密度 | 1.4±0.1 g/cm3 | 沸点 | 584.0±60.0 °C at 760 mmHg | |
| 分子式 | C21H15F3N2O2S | 熔点 | N/A | |
| MSDS | 美版 | 闪点 | 307.0±32.9 °C |
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Positively charged mini-protein Zbasic2 as a highly efficient silica binding module: opportunities for enzyme immobilization on unmodified silica supports.
Langmuir 28(26) , 10040-9, (2012) Silica is a highly attractive support material for protein immobilization in a wide range of biotechnological and biomedical-analytical applications. Without suitable derivatization, however, the silica surface is not generally usable for attachment of protei... |
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Phosphorolytic cleavage of sucrose by sucrose-grown ruminal bacterium Pseudobutyrivibrio ruminis strain k3.
Folia Microbiol. (Praha) 55(4) , 383-5, (2010) Rumen bacterium Pseudobutyrivibrio ruminis strain k3 utilized over 90% sucrose added to the growth medium as a sole carbon source. Zymographic studies of the bacterial cell extract revealed the presence of a single enzyme involved in sucrose digestion. Thin l... |
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Enzymatic synthesis of stable, odorless, and powdered furanone glucosides by sucrose phosphorylase.
Biosci. Biotechnol. Biochem. 64 , 134, (2000) Sucrose phosphorylase from Leuconostoc mesenteroides catalyzed transglucosylation from sucrose to 4-hydroxy-3(2H)-furanone derivatives. When 4-hydroxy-2,5-dimethyl-3(2H)-furanone (HDMF) and 2-ethyl-4-hydroxy-5-methyl-3(2H)-furanone or 5-ethyl-4-hydroxy-2-meth... |
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Enzymatic synthesis of sucrose and other disaccharides.
Adv. Carbohydr. Chem. 5 , 29-48, (1950)
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Single-step enzymatic synthesis of (R)-2-O-alpha-D-glucopyranosyl glycerate, a compatible solute from micro-organisms that functions as a protein stabiliser.
Org. Biomol. Chem. 7(20) , 4267-70, (2009) Regioselective glucosylation of R-glycerate catalysed by sucrose phosphorylase in the presence of sucrose as the donor substrate provided the natural compatible solute (R)-2-O-alpha-D-glucopyranosyl glycerate with complete regioselectivity in an optimised syn... |
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Enzyme assays with boronic acid appended bipyridinium salts.
Anal. Chim. Acta 649(2) , 246-51, (2009) In-vitro fluorescent enzyme assays have been developed for sucrose phosphorylase (SPO) and phosphoglucomutase (PGM). These assays make use of a selective carbohydrate sensing system that detects the unlabeled enzymatic products fructose and glucose-6-phosphat... |
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Sucrose phosphorylase harbouring a redesigned, glycosyltransferase-like active site exhibits retaining glucosyl transfer in the absence of a covalent intermediate.
ChemBioChem. 10(14) , 2333-7, (2009)
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Aromatic interactions at the catalytic subsite of sucrose phosphorylase: their roles in enzymatic glucosyl transfer probed with Phe52→Ala and Phe52→Asn mutants.
FEBS Lett. 585(3) , 499-504, (2011) Mutants of Leuconostoc mesenteroides sucrose phosphorylase having active-site Phe(52) replaced by Ala (F52A) or Asn (F52N) were characterized by free energy profile analysis for catalytic glucosyl transfer from sucrose to phosphate. Despite large destabilizat... |
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Small-molecule glucosylation by sucrose phosphorylase: structure-activity relationships for acceptor substrates revisited.
Carbohydr. Res. 345(10) , 1492-6, (2010) Sucrose phosphorylase catalyzes the O-glucosylation of a wide range of acceptor substrates. Acceptors presenting a suitable 1,2-diol moiety are glucosylated exclusively at the secondary hydroxyl. Production of the naturally occurring compatible solute, 2-O-al... |
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Glucosylation of acetic acid by sucrose phosphorylase.
Biosci. Biotechnol. Biochem. 72(1) , 82-7, (2008) Transglucosylation from sucrose to acetic acid by sucrose phosphorylase (EC 2.4.1.7) was studied. 1-O-Acetyl-alpha-D-glucopyranose was isolated as the main product of the enzyme reaction. We also compared the pH-dependence of transglycosylation catalyzed by s... |