NU6027
NU6027 structure
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Common Name | NU6027 | ||
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| CAS Number | 220036-08-8 | Molecular Weight | 251.285 | |
| Density | 1.5±0.1 g/cm3 | Boiling Point | 549.2±60.0 °C at 760 mmHg | |
| Molecular Formula | C11H17N5O2 | Melting Point | 252.5-253.7 °C(lit.) | |
| MSDS | Chinese USA | Flash Point | 286.0±32.9 °C | |
Use of NU6027NU6027 is a potent and ATP-competitive inhibitor of both CDK1 and CDK2, with Kis of 2.5 µM and 1.3 µM, respectively. NU6027 is also a potent inhibitor of ATR and enhances hydroxyurea and cisplatin cytotoxicity in an ATR-dependent manner[1][2]. |
| Name | 6-(cyclohexylmethoxy)-5-nitrosopyrimidine-2,4-diamine |
|---|---|
| Synonym | More Synonyms |
| Description | NU6027 is a potent and ATP-competitive inhibitor of both CDK1 and CDK2, with Kis of 2.5 µM and 1.3 µM, respectively. NU6027 is also a potent inhibitor of ATR and enhances hydroxyurea and cisplatin cytotoxicity in an ATR-dependent manner[1][2]. |
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| Related Catalog | |
| Target |
CDK1:2.5 μM (Ki) CDK2:1.3 μM (Ki) ATR |
| In Vitro | NU6027 (1 nM-100 µM; 48 h) inhibits the growth of human tumor cells with a GI50 of 10±6 µM[1]. NU6027 (0.1-25 µM; 24 h) inhibits ATR activity with an IC50 of 2.8 µM in GM847KD cells. NU6027 (1-10 µM; 24 h) inhibits ATR activity with an IC50 of 6.7±2.3 µM in MCF7 cells[2]. NU6027 (4 or 10 µM; 24 h) attenuates G2/M arrest following DNA damage in MCF7 cells[2]. NU6027 (10 µM; 24 h) significantly reduces RAD51 foci in both control and PF-01367338-treated V-C8 B2 cells[2]. NU6027 (4 µM; 24 h) causes 82% suppression of the increase in RAD51 foci-positive cells treated by PF-01367338[2]. Western Blot Analysis[2] Cell Line: MCF7 cells Concentration: 0, 1, 5, 10 μM Incubation Time: 24 h Result: Inhibited CDK2-mediated pRbT821 by 42±27% compared with 70±12% inhibition of pCHK1S345 with the concentration of 10 µM. |
| References |
| Density | 1.5±0.1 g/cm3 |
|---|---|
| Boiling Point | 549.2±60.0 °C at 760 mmHg |
| Melting Point | 252.5-253.7 °C(lit.) |
| Molecular Formula | C11H17N5O2 |
| Molecular Weight | 251.285 |
| Flash Point | 286.0±32.9 °C |
| Exact Mass | 251.138229 |
| PSA | 117.21000 |
| LogP | 3.71 |
| Appearance of Characters | lavender |
| Vapour Pressure | 0.0±1.5 mmHg at 25°C |
| Index of Refraction | 1.699 |
| Storage condition | 2-8°C |
| Water Solubility | DMSO: 15 mg/mL |
| Personal Protective Equipment | Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter |
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| Hazard Codes | Xi |
| RIDADR | NONH for all modes of transport |
| WGK Germany | 3 |
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Ataxia telangiectasia mutated and Rad3 related (ATR) protein kinase inhibition is synthetically lethal in XRCC1 deficient ovarian cancer cells.
PLoS ONE 8 , e57098, (2013) Ataxia telangiectasia mutated and Rad3 Related (ATR) protein kinase is a key sensor of single-stranded DNA associated with stalled replication forks and repair intermediates generated during DNA repai... |
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Identification and evaluation of a potent novel ATR inhibitor, NU6027, in breast and ovarian cancer cell lines.
Br. J. Cancer 105 , 372-81, (2011) The ataxia telangiectasia mutated and Rad3-related kinase (ATR) has a key role in the signalling of stalled replication forks and DNA damage to cell cycle checkpoints and DNA repair. It has long been ... |
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Kinetically-defined component actions in gene repression.
PLoS Comput. Biol. 11(3) , e1004122, (2015) Gene repression by transcription factors, and glucocorticoid receptors (GR) in particular, is a critical, but poorly understood, physiological response. Among the many unresolved questions is the diff... |
| 6-(Cyclohexylmethoxy)-5-nitroso-2,4-pyrimidinediamine |
| 4-cyclohexylmethoxy-5-nitrosopyrimidine-2,6-diamine |
| 2,6-Diamino-4-cyclohexylmethoxy-5-nitroso pyrimidine |
| NU6027 |
| NW1 |
| 1e1x |
| 6-CYCLOHEXYLMETHYLOXY-5-NITROSO-PYRIMIDINE-2,4-DIAMINE |
| 2,4-Pyrimidinediamine, 6-(cyclohexylmethoxy)-5-nitroso- |