Pyrromethene 546
Pyrromethene 546 structure
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Common Name | Pyrromethene 546 | ||
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| CAS Number | 121207-31-6 | Molecular Weight | 262.10600 | |
| Density | N/A | Boiling Point | N/A | |
| Molecular Formula | C14H17BF2N2 | Melting Point | 257 °C | |
| MSDS | USA | Flash Point | N/A | |
| Symbol |
GHS07 |
Signal Word | Warning | |
Use of Pyrromethene 546BODIPY 493/503 (Pyrromethene 546) is a fluorescent neutral lipid dye (Ex/Em: 493/503 nm). BODIPY 493/503 can facilitate quantification of neutral lipid content by flow cytometry and observation of lipid droplets (LDs) by microscopy[1]. |
This table lists Chinese names, IUPAC names and various aliases of this chemical substance.
| Name | 4,4-Difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene |
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| Synonym | More Synonyms |
This table contains bioactivity, target information and biological‑assay experimental data of this compound.
| Description | BODIPY 493/503 (Pyrromethene 546) is a fluorescent neutral lipid dye (Ex/Em: 493/503 nm). BODIPY 493/503 can facilitate quantification of neutral lipid content by flow cytometry and observation of lipid droplets (LDs) by microscopy[1]. |
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| Related Catalog | |
| In Vitro | Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). A. BODIY staining for flow cytometry 1. Grow cells under culture conditions relevant for the study. 2. At the time-point of interest, prepare 2 μM BODIPY staining solution in PBS. The volume of staining solution required for each sample corresponds to the volume of media used for culturing cells. 3. Wash cells with a quick rinse using 3 mL PBS to remove media/serum. 4. Incubate on BODIPY staining solution in the dark for 15 min at 37°C. Include an unstained control for flow cytometry. (Note: From this point, protect samples from light as much as possible) 5. Wash cells with a quick rinse using 3 mL PBS to remove staining solution. 6. Trypsinize cells to generate a single cell suspension. 7. Add 5 mL of PBS and transfer cell suspension to a 15 mL conical tube. 8. Pellet cells at 250×g, 5 min, 4 °C. 9. Aspirate supernatant, wash the cell pellet with a quick rinse using 3 mL PBS, and pellet cells at 250×g, 5 min, 4°C. 10. Carefully aspirate the supernatant and resuspend cells in 300 μL 1×flow cytometry buffer. 11. Pass cell suspension through a 35 μM filter into a FACS tube. 12. Perform flow cytometry. Obtain a minimum of 10,000 events per condition. 13. The investigator can analyze data as mean fluorescence or display the data as a histogram[1]. B. BODIY staining for microscopy 1. Autoclave coverslips in a glass bottle. 2. In the tissue culture hood, place coverslips into 35 mm cell culture dishes. 3. Prepare 2 mg/mL collagen solution in PBS. 4. Treat the coverslips with collagen to promote cell adherence. Add 3 mL collagen solution to culture dishes and incubate at 37°C for 30 min. Note: Use forceps to ensure that coverslips are flush with the bottom of the culture dish, eliminating any air bubbles that may be under the cover slips. 5. Aspirate the collagen solution. 6. Wash with PBS. 7. Add PBS to culture dishes and place under UV light in the culture hood to sterilize. 8. Plate cells into culture dishes containing the coverslips. The optimal cell number should be determined to achieve confluence of 30-50% at the time of staining to permit proper imaging. 9. Incubate under the culture conditions relevant to your experiment. 10. At the time-point of interest, prepare 2 μM BODIPY staining solution in PBS. 11. Wash cells with 3 mL PBS. 12. Incubate on 3 mL staining solution for 15 min at 37 °C. (Note: From this point, protect samples from light as much as possible) 13. Wash twice in 3 mL PBS. 14. Fix cells in 3 mL 4% PFA for 30 min at room temperature. 15. Remove 4% PFA. 16. Wash samples 3×5 min in PBS. 17. Use forceps to mount cover slips onto glass slides. 18. Allow the mounting solution to cure overnight at room temperature. 19. Slides can be stored at 4°C or imaged immediately[1]. |
| References |
This table shows physicochemical parameters including melting point, boiling point, density, solubility and optical rotation. Missing data is marked as N/A.
| Melting Point | 257 °C |
|---|---|
| Molecular Formula | C14H17BF2N2 |
| Molecular Weight | 262.10600 |
| Exact Mass | 262.14500 |
| PSA | 13.32000 |
| LogP | 2.82650 |
| InChIKey | DRJHPEGNOPSARR-UHFFFAOYSA-N |
| SMILES | CC1=CC(C)=[N+]2C1=C(C)c1c(C)cc(C)n1[B-]2(F)F |
This table provides MSDS information including hazard classification, first‑aid, fire‑fighting, spill handling, operation and storage instructions.
This table covers safety warnings, protective measures, incompatible substances and disposal guidelines for this compound.
| Symbol |
GHS07 |
|---|---|
| Signal Word | Warning |
| Hazard Statements | H315-H319-H335 |
| Precautionary Statements | P261-P305 + P351 + P338 |
| RIDADR | NONH for all modes of transport |
This table presents publicly reported synthetic routes, reaction conditions, reactants and product information of the compound.
![5-fluoro-5-(fluoro-18F)-1,3,7,9,10-pentamethyl-5H-54-dipyrrolo[1,2-c:2',1'-f][1,3,2]diazaborinin-4-ium structure](https://image.chemsrc.com/caspic/107/1380420-29-0.png)
This table lists upstream starting materials and downstream derivative products related to this chemical.
| Precursor 3 | |
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| DownStream 0 | |
This table lists relevant public references with title, journal and publication metadata for this compound.
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MicroRNA-195 inhibits proliferation, invasion and metastasis in breast cancer cells by targeting FASN, HMGCR, ACACA and CYP27B1.
Sci. Rep. 5 , 17454, (2015) De novo lipogenesis, a hallmark for cancers is required for cellular transformation. Further it is believed that resistance to apoptosis and epithelial-to-mesenchymal-transition(EMT) facilitates metas... |
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Retinoic acid induces macrophage cholesterol efflux and inhibits atherosclerotic plaque formation in apoE-deficient mice.
Br. J. Nutr. 114 , 509-18, (2015) It has been suggested that retinoic acid (RA) has a potential role in the prevention of atherosclerotic CVD. In the present study, we used J774A.1 cell lines and primary peritoneal macrophages to inve... |
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A new route towards colloidal molecules with externally tunable interaction sites.
Faraday Discuss. 181 , 49-69, (2015) We describe a route towards self-assembled colloidal molecules, where thermoresponsive microgels serve as discrete, externally tunable interaction sites. The ability of poly(N-isopropylacrylamide) (PN... |
This table contains target information, in‑vitro & in‑vivo assay data for this compound.
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Name: Drug uptake in human HeLa cells infected with Chlamydia trachomatis serovar LGV-L2 at...
Source: ChEMBL
Target: HeLa
External Id: CHEMBL3796918
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Name: Drug uptake in Chlamydia trachomatis serovar LGV-L2 inclusion infected in human HeLa ...
Source: ChEMBL
Target: Chlamydia trachomatis
External Id: CHEMBL3796917
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This table collects all English aliases, systematic names and CAS‑related synonyms of the compound.
| 1,3,5,7,8-pentamethyl-4,4-difluoro-4-bora-3a,4a-diaza-s-indacene |
| 4,4-difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a,diaza-s-indacene |
| Difluoro{2-[1-(3,5-dimethyl-2H-pyrrol-2-ylidene-N)ethyl]-3,5-dimethyl-1H-pyrrolato-N}boron |
| 4,4-difluoro-1,3,5,7,8-pentamethyl-BODIPY |
| Pyrromethene 546 |
| 4,4-difluoro-1,3,4,5,7-quantmethyl-4-bora-3a,4a-diaza-s-indacene |
| BODIPY 493/503 |
This section contains frequently‑asked‑questions about this compound, including basic parameters, properties, storage conditions and corresponding answers.
| Q: What is the InChIKey of 4,4-Difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene? |
| A: InChIKey of 4,4-Difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene is DRJHPEGNOPSARR-UHFFFAOYSA-N. |
| Q: What is the LogP of 4,4-Difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene? |
| A: LogP of 4,4-Difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene is 2.82650. |
| Q: What is the CAS number of 4,4-Difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene? |
| A: CAS number of 4,4-Difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene is 121207-31-6. |
| Q: What is the molecular weight of 4,4-Difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene? |
| A: Molecular weight of 4,4-Difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene is 262.10600. |
| Q: What are the uses of 4,4-Difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene? |
| A: Main uses of 4,4-Difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene: BODIPY 493/503 (Pyrromethene 546) is a fluorescent neutral lipid dye (Ex/Em: 493/503 nm). BODIPY 493/503 can facilitate quantification of neutral lipid content by flow cytometry and observation of lipid droplets (LDs) by microscopy[1]. |
| Q: What are the upstream materials of 4,4-Difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene? |
| A: Related upstream materials(CAS) of 4,4-Difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene: 625-82-1;109-63-7;75-36-5. |
| Q: What is the polar surface area (PSA) of 4,4-Difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene? |
| A: Polar surface area (PSA) of 4,4-Difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene is 13.32000. |
| Q: What is the SMILES notation of 4,4-Difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene? |
| A: SMILES notation of 4,4-Difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene is CC1=CC(C)=[N+]2C1=C(C)c1c(C)cc(C)n1[B-]2(F)F. |
| Q: What is the molecular formula of 4,4-Difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene? |
| A: Molecular formula of 4,4-Difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene is C14H17BF2N2. |
| Q: What is the melting point of 4,4-Difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene? |
| A: Melting point of 4,4-Difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene is 257 °C. |
This table lists manufacturers and suppliers of this compound, including vendor names and product supply reference information.
| Shanghai Nianxing Industrial Co., Ltd |
| Dayang Chem (Hangzhou) Co., Ltd. |