Alternatives to Laboratory Animals 2015-03-01

Toxicity assessment of tobacco products in vitro.

Joseph R Manuppello, Kristie M Sullivan

Index: Altern. Lab. Anim. 43 , 39-67, (2015)

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Abstract

Driven by new regulatory demands to demonstrate risk reduction, the toxicity assessment of tobacco products increasingly employs innovative in vitro methods, including biphasic cell and tissue cultures exposed to whole cigarette smoke at the air-liquid interface, cell transformation assays, and genomic analyses. At the same time, novel tobacco products are increasingly compared to traditional cigarettes. This overview of in vitro toxicology studies of tobacco products reported in the last five years provides evidence to support the prioritisation of in vitro over in vivo methods by industry and their recommendation by regulatory authorities. 2015 FRAME.