Nucleic Acid Therapeutics 2015-06-01

Turning Squalene into Cationic Lipid Allows a Delivery of siRNA in Cultured Cells.

Jean-Rémi Bertrand, Claire Lucas, Ngoc Minh Pham, Catherine Durieu, Patrick Couvreur, Claude Paul Malvy, Didier Desmaële

Index: Nucleic Acid Ther. 25 , 121-9, (2015)

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Abstract

Covalent binding of squalene to siRNA has already been shown to be an interesting way of delivering siRNA in vivo. Whether squalene derivatives could also be used to deliver siRNA in cells without covalent binding similar to usual transfection with cationic lipids is the question addressed in this article. Accordingly, we investigated the activity of two squalene derivatives bearing a quaternary ammonium head group and a guanidinium group, respectively. The second derivative displayed interesting properties for delivering siRNA into cells in vitro.