L-(+)-Ribulose

L-(+)-Ribulose Structure
L-(+)-Ribulose structure
Common Name L-(+)-Ribulose
CAS Number 527-50-4 Molecular Weight 150.130
Density 1.7±0.1 g/cm3 Boiling Point 364.2±42.0 °C at 760 mmHg
Molecular Formula C5H10O5 Melting Point N/A
MSDS Chinese USA Flash Point 174.1±27.9 °C

Construction and co-expression of plasmid encoding xylitol dehydrogenase and a cofactor regeneration enzyme for the production of xylitol from D-arabitol.

Enzyme Microb. Technol. 51(2) , 119-24, (2012)

The biotransformation of D-arabitol into xylitol was investigated with focus on the conversion of D-xylulose into xylitol. This critical conversion was accomplished using Escherichia coli to co-express a xylitol dehydrogenase gene from Gluconobacter oxydans a...

Cloning and characterization of a novel NAD+ -dependent xylitol dehydrogenase from Gluconobacter oxydans CGMCC 1. 637.

Wei Sheng Wu Xue Bao 52(6) , 726-35, (2012)

To clone the xylitol dehydrogenase gene from Gluconobacter oxydans CGMCC 1.637, to characterize enigmatic properties of xylitol dehydrogenase and to investigate the induction abilities of various carbon sources on the oxidative activity of xylitol dehydrogena...

Gene cloning and characterization of L-ribulose 3-epimerase from Mesorhizobium loti and its application to rare sugar production.

Biosci. Biotechnol. Biochem. 77(3) , 511-5, (2013)

A gene encoding L-ribulose 3-epimerase (L-RE) from Mesorhizobium loti, an important enzyme for rare sugar production by the Izumoring strategy, was cloned and overexpressed. The enzyme showed highest activity toward L-ribulose (230 U/mg) among keto-pentoses a...

Insights into the isomerization of xylose to xylulose and lyxose by a Lewis acid catalyst.

Carbohydr. Res. 368 , 89-95, (2013)

We present electronic structure calculations on the isomerization and epimerization of xylose to xylulose and lyxose, respectively, by a zeolite Sn-BEA model at the MP2 and B3LYP theory levels. Benchmarking calculations at the CCSD(T) theory level are also pr...

Alteration of xylose reductase coenzyme preference to improve ethanol production by Saccharomyces cerevisiae from high xylose concentrations.

Bioresour. Technol. 102(19) , 9206-15, (2011)

A K270R mutation of xylose reductase (XR) was constructed by site-direct mutagenesis. Fermentation results of the F106X and F106KR strains, which carried wild type XR and K270R respectively, were compared using different substrate concentrations (from 55 to 2...

Covalent immobilization of recombinant Rhizobium etli CFN42 xylitol dehydrogenase onto modified silica nanoparticles.

Appl. Microbiol. Biotechnol. 90(2) , 499-507, (2011)

Rare sugars have many applications in food industry, as well as pharmaceutical and nutrition industries. Xylitol dehydrogenase (XDH) can be used to synthesize various rare sugars enzymatically. However, the immobilization of XDH has not been performed to impr...

Using neutron protein crystallography to understand enzyme mechanisms.

Acta Crystallogr. D Biol. Crystallogr. 66(Pt 11) , 1257-61, (2010)

A description is given of the results of neutron diffraction studies of the structures of four different metal-ion complexes of deuterated D-xylose isomerase. These represent four stages in the progression of the biochemical catalytic action of this enzyme. A...

Characterization of H2O-forming NADH oxidase from Streptococcus pyogenes and its application in l-rare sugar production.

Bioorg. Med. Chem. Lett. 22(5) , 1931-5, (2012)

A nicotinamide adenine dinucleotide (NADH) oxidase from Streptococcus pyogenes MGAS10394 (SpNox) was cloned and overexpressed in Escherichia coli BL21 (DE3). The purified SpNox enzyme had optimal pH and temperature of 7.0 and 55°C, respectively, with a K(m) o...

A viable method and configuration for fermenting biomass sugars to ethanol using native Saccharomyces cerevisiae.

Bioresour. Technol. 117 , 92-8, (2012)

A system that incorporates a packed bed reactor for isomerization of xylose and a hollow fiber membrane fermentor (HFMF) for sugar fermentation by yeast was developed for facile recovery of the xylose isomerase enzyme pellets and reuse of the cartridge loaded...

Production ofl-xylose froml-xylulose usingEscherichia colil-fucose isomerase

Enzyme Microb. Technol. 50(1) , 71-6, (2012)

Highlights ► l-Fucose isomerase catalyzed isomerization of l-xylulose to l-xylose does not produce l-lyxose. ► New characteristics on the catalytic mechanisms related to l-fucose isomerase are presented. ► A potential pathway for the production of the pharmac...