2-Nitrophenylhydrazine
2-Nitrophenylhydrazine structure
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Common Name | 2-Nitrophenylhydrazine | ||
|---|---|---|---|---|
| CAS Number | 3034-19-3 | Molecular Weight | 153.13900 | |
| Density | N/A | Boiling Point | N/A | |
| Molecular Formula | C6H7N3O2 | Melting Point | 91-93 °C(lit.) | |
| MSDS | Chinese USA | Flash Point | N/A | |
| Symbol |
GHS02, GHS07 |
Signal Word | Danger | |
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Liquid chromatographic determination of free and total fatty acids in milk and milk products as their 2-nitrophenylhydrazides.
J. Chromatogr. A. 523 , 235-46, (1990) A high-performance liquid chromatographic (HPLC) method with direct derivatization is described for the determination of both free fatty acids and total fatty acids in milk and milk products. The method is based on the reaction of these acids with 2-nitrophen... |
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Determination of methyl ethyl ketone in human urine after derivatization with o-nitrophenylhydrazine, using solid-phase extraction and reversed-phase high-performance liquid chromatography and ultraviolet detection.
J. Chromatogr. A. 489(2) , 419-24, (1989)
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On-line determination of carboxylic acids, aldehydes and ketones by high-performance liquid chromatography-diode array detection-atmospheric pressure chemical ionisation mass spectrometry after derivatization with 2-nitrophenylhydrazine.
J. Chromatogr. A. 1031(1-2) , 35-50, (2004) 2-Nitrophenylhydrazine (2-NPH) is widely used for the derivatization of carboxylic acids, aldehydes and ketones, in industrial and biological samples. These compounds react with 2-NPH to form derivatives, which are separated by high-performance liquid chromat... |
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High-performance liquid chromatographic analysis of fatty acid compositions of platelet phospholipids as their 2-nitrophenylhydrazides.
J. Chromatogr. A. 568(1) , 25-34, (1991) A novel high-performance liquid chromatographic method for biologically important fatty acids incorporated into platelet phospholipids in esterified form has been developed. 2-Nitrophenylhydrazine hydrochloride was used as a pre-column labelling agent to conv... |
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Antigenic determinants of Staphylococcus aureus type 5 and type 8 capsular polysaccharide vaccines.
Infect. Immun. 66(10) , 4588-92, (1998) Bacterial capsular polysaccharides (CP) are carbohydrate polymers comprised of repeating saccharide units. Several of these CP have side chains attached to their backbone structures. The side chains may include O-acetyl, phosphate, sialic acid, and other moie... |
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Mass spectrometry analysis of 2-nitrophenylhydrazine carboxy derivatized peptides.
J. Am. Soc. Mass Spectrom. 22(11) , 1958-67, (2011) Peptides with two or more basic residues, including those with post-translational modifications (PTMs), such as methylation and phosphorylation, can be highly hydrophilic and, therefore, are often difficult to be retained on a reversed-phase (RP) column. In a... |
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Determination of biotin following derivatization with 2-nitrophenylhydrazine by high-performance liquid chromatography with on-line UV detection and electrospray-ionization mass spectrometry.
J. Chromatogr. A. 1142(2) , 231-5, (2007) Currently, biotin is typically determined in Japan using a microbiological method. Such microbiological assays are sensitive, but they are not always highly specific and are also rather tedious and time-consuming. In the present study, RP-HPLC and LC-MS metho... |
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Physical, chemical, antigenic, and immunologic characterization of polygalacturonan, its derivatives, and Vi antigen from Salmonella typhi.
Meth. Enzymol. 363 , 552-67, (2003)
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High-performance liquid chromatographic analyses of hydroxymonocarboxylic acids and dicarboxylic acids in urine as their 2-nitrophenylhydrazides.
Anal. Biochem. 185(1) , 17-23, (1990) Both hydroxymonocarboxylic acids and dicarboxylic acids in urine were converted into their 2-nitrophenylhydrazides without lengthy and cumbersome sample workup and were separated from each other by two-step extraction with diethyl ether at different pH values... |
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Separation of positional and geometrical fatty acid isomers as 2-nitrophenylhydrazide derivatives by high-performance liquid chromatography.
Anal. Biochem. 413(1) , 66-8, (2011) Previously, following derivatization to their 2-nitrophenylhydrazide (2-NPH) derivatives, fatty acid (FA) abundances have been evaluated using high-performance liquid chromatography (HPLC). Although the method was sensitive, resolution was insufficient for ma... |