(4-Methylumbelliferone)-β-D-xylopyranoside

(4-Methylumbelliferone)-β-D-xylopyranoside Structure
(4-Methylumbelliferone)-β-D-xylopyranoside structure
Common Name (4-Methylumbelliferone)-β-D-xylopyranoside
CAS Number 6734-33-4 Molecular Weight 308.283
Density 1.5±0.1 g/cm3 Boiling Point 572.1±50.0 °C at 760 mmHg
Molecular Formula C15H16O7 Melting Point 223ºC
MSDS USA Flash Point 217.3±23.6 °C

Mechanistic studies of active site mutants of Thermomonospora fusca endocellulase E2.

Biochemistry 38(30) , 9746-51, (1999)

Endocellulase E2 from the thermophilic bacterium Thermomonospora fusca is a member of glycosyl-hydrolase family 6 and is active from pH 4 to 10. Enzymes in this family hydrolyze beta-1,4-glycosidic bonds with inversion of the stereochemistry at the anomeric c...

A sulfated proteoglycan is necessary for storage of exocrine secretory proteins in the rat parotid gland.

Am. J. Physiol. Cell Physiol. 283(2) , C438-45, (2002)

Sulfated proteoglycans have been proposed to play a role in the sorting and storage of secretory proteins in exocrine secretory granules. Rat parotid acinar cells expressed a 40- to 60-kDa proteoglycan that was stored in secretory granules. Treatment of the t...

Simple measurement of glycosaminoglycan produced by cultured fibroblasts using 4-methylumbelliferyl beta-D-xyloside.

J. Biochem. Biophys. Methods 26(4) , 309-16, (1993)

A simple and rapid method was devised for measurement of glycosaminoglycan produced by cultured cells. 4-Methylumbelliferyl-beta-D-xyloside was added to the medium of the cultured cells. After incubation, glycosaminoglycan, which was produced from 4-methylumb...

Sulphation of proteochondroitin and 4-methylumbelliferyl beta-D-xyloside-chondroitin formed by mouse mastocytoma cells cultured in sulphate-deficient medium.

Biochem. J. 296 ( Pt 1) , 119-26, (1993)

Mouse mastocytoma cells were cultured in medium containing [3H]GlcN and concentrations of [35S]sulphate varying from 0.01 to 0.5 mM. Intracellular [35S]sulphate incorporation increased severalfold from the lowest concentrations, reaching a maximum at 0.1-0.2 ...

Inhibition of proteoglycan synthesis influences regeneration of goldfish retinal axons on polylysine and laminin.

Exp. Neurol. 134(1) , 126-34, (1995)

Previous studies have shown that goldfish retinal axons regenerating in vivo transport increased radioactivity in the glycosaminoglycan (GAG) components of proteoglycans (PGs). During this enhanced transport, the ratio of chondroitin sulfate (CS) to heparan s...

A novel oligosaccharide, xylosyl beta 1-4xylosyl beta 1-(4-methylumbelliferone), synthesized by cultured human skin fibroblasts in the presence of 4-methylumbelliferyl-beta-D-xyloside.

J. Biochem. 116(3) , 524-9, (1994)

4-Methylumbelliferyl-beta-D-xyloside (Xyl-MU) was added to the medium of cultured human skin fibroblasts. After incubation, the culture medium was pooled, concentrated with a lyophilizer, and dialyzed against distilled water. Then the Xyl-MU derivatives in th...

A new approach to mapping co-localization of multiple glycosyl transferases in functional Golgi preparations.

Glycobiology 6(2) , 177-89, (1996)

We have developed a new method to co-localize multiple glycosyl transferases in different Golgi compartments. The approach relies on the proven ability of intact, sealed rat liver Golgi preparations to concentrate exogenous labeled sugar nucleotides into the ...

Characterization and biological significance of sialyl alpha 2-3galactosyl beta 1-4xylosyl beta 1-(4-methylumbelliferone) synthesized in cultured human skin fibroblasts.

J. Biochem. 119(4) , 697-702, (1996)

Human skin fibroblasts were incubated in the presence of a fluorogenic xyloside, 4-methyl-umbelliferyl-beta-D-xyloside (Xyl-MU), then the cultured medium was recovered, concentrated with a lyophilizer, and dialyzed against distilled water. The structures of t...

Alterations of matrix- and cell-associated proteoglycans inhibit osteogenesis and growth response to fibroblast growth factor-2 in cultured rat mandibular condyle and calvaria.

Cell Tissue Res. 295(3) , 523-36, (1999)

Matrix and cell surface proteoglycans (PGs) may play important roles in the control of cellular actions of heparan-binding growth factors such as fibroblast growth factor (FGF) during chondrogenesis and osteogenesis. In this study, we used 4-methylumbellifery...

Effect of monensin on the synthesis of beta-D-xyloside-initiated glycosaminoglycan and its linkage region oligosaccharides in human skin fibroblasts.

J. Biochem. 122(6) , 1129-32, (1997)

Human skin fibroblasts were cultured with a fluorogenic xyloside, 4-methylumbelliferyl-beta-D-xyloside (Xyl-MU) as an initiator, and the effects of monensin, which destroys the normal structure of the Golgi complex, on the synthesis of Xyl-MU-initiated glycos...