Oxonol VI

Oxonol VI Structure
Oxonol VI structure
Common Name Oxonol VI
CAS Number 64724-75-0 Molecular Weight 316.35200
Density 1.19g/cm3 Boiling Point 381.4ºC at 760 mmHg
Molecular Formula C17H20N2O4 Melting Point N/A
MSDS Chinese USA Flash Point 184.5ºC

Conserved amino acid residues of the NuoD segment important for structure and function of Escherichia coli NDH-1 (complex I).

Biochemistry 54(3) , 753-64, (2015)

The NuoD segment (homologue of mitochondrial 49 kDa subunit) of the proton-translocating NADH:quinone oxidoreductase (complex I/NDH-1) from Escherichia coli is in the hydrophilic domain and bears many highly conserved amino acid residues. The three-dimensiona...

ATP-dependent spectral response of oxonol VI in an ATP-Pi exchange complex.

Biochim. Biophys. Acta 766(2) , 375-85, (1984)

Energy transduction in an ATPase complex (complex V) has been studied in two reactions catalyzed by this system, i.e., ATP-dependent spectral shift of oxonol VI, and ATP-Pi exchange activity. Aurovertin alone inhibits 50% of the oxonol shift at 2 microM, and ...

Effects of Cd2+ on ATP-driven membrane potential in beef heart mitochondrial H+-ATPase: a study using the voltage-sensitive probe oxonol VI.

Membr. Biochem. 5(3) , 225-41, (1984)

Beef heart mitochondrial H+-ATPase (F1-F0) vesicles were prepared by lysolecithin extraction of ETPH. ATP-driven membrane potential was monitored indirectly by following absorbance changes of the potential-sensitive dye oxonol VI. The steady-state potential w...

Na+-pyrophosphatase: a novel primary sodium pump.

Biochemistry 46(30) , 8872-8, (2007)

Membrane-bound pyrophosphatase (PPase) is commonly believed to couple pyrophosphate (PPi) hydrolysis to H+ transport across the membrane. Here, we demonstrate that two newly isolated bacterial membrane PPases from the mesophile Methanosarcina mazei (Mm-PPase)...

A stopped-flow kinetic study of the interaction of potential-sensitive oxonol dyes with lipid vesicles.

Biophys. Chem. 34(3) , 225-37, (1989)

The interaction of the dyes oxonol V and oxonol VI with unilamellar dioleoylphosphatidylcholine vesicles was investigated using a fluorescence stopped-flow technique. On mixing with the vesicles, both dyes exhibit an increase in their fluorescence, which occu...

Electrophysiological study with oxonol VI of passive NO3- transport by isolated plant root plasma membrane.

Biophys. J. 76(1 Pt 1) , 360-73, (1999)

In contrast to animal cells, plant cells contain approximately 5-50 mM nitrate in cytosol and vacuole. The lack of specific spectroscopic probes, or suitable isotopes, impedes in vitro studies of NO3- transport. Reconstitution of root cell plasma membrane (PM...

The use of carotenoids and oxonol VI as probes for membrane potential in proteoliposomes.

FEBS Lett. 184(1) , 96-9, (1985)

Carotenoids present in lipids extracted from the cyanobacterium Synechococcus 6716 indicate trans-membrane potential in proteoliposomes reconstituted from these lipids and the ATPase complex isolated from the same organism. A carotenoid absorbance band shift ...

Evidence for a selective and electroneutral K+/H(+)-exchange in Saccharomyces cerevisiae using plasma membrane vesicles.

Yeast 12(13) , 1301-13, (1996)

The existence of a K+/H+ transport system in plasma membrane vesicles from Saccharomyces cerevisiae is demonstrated using fluorimetric monitoring of proton fluxes across vesicles (ACMA fluorescence quenching). Plasma membrane vesicles used for this study were...

Indications for acceleration-dependent changes of membrane potential in the flagellate Euglena gracilis.

Protoplasma 229(2-4) , 101-8, (2006)

The effects of the calcium sequester EGTA on gravitactic orientation and membrane potential changes in the unicellular flagellate Euglena gracilis were investigated during a recent parabolic-flight experiment aboard of an Airbus A300. In the course of a fligh...

Physiological parameters of gravitaxis in the flagellate Euglena gracilis obtained during a parabolic flight campaign.

J. Plant Physiol. 159(2) , 181-90, (2002)

The unicellular freshwater flagellate Euglena gracilis and its close relative Astasia longa show a pronounced negative gravitaxis. Previous experiments revealed that gravitaxis is most likely mediated by an active physiological mechanism in which changes of t...