P-NITROPHENYL PHOSPHATE MAGNESIUM SALT
P-NITROPHENYL PHOSPHATE MAGNESIUM SALT structure
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Common Name | P-NITROPHENYL PHOSPHATE MAGNESIUM SALT | ||
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| CAS Number | 32348-90-6 | Molecular Weight | 243.393 | |
| Density | N/A | Boiling Point | N/A | |
| Molecular Formula | C6H6MgNO6P | Melting Point | N/A | |
| MSDS | N/A | Flash Point | N/A | |
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Site-directed mutagenesis maps interactions that enhance cognate and limit promiscuous catalysis by an alkaline phosphatase superfamily phosphodiesterase.
Biochemistry 52(51) , 9167-76, (2013) Catalytic promiscuity, an evolutionary concept, also provides a powerful tool for gaining mechanistic insights into enzymatic reactions. Members of the alkaline phosphatase (AP) superfamily are highly amenable to such investigation, with several members havin... |
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Kinetic behaviour of calf intestinal alkaline phosphatase with pNPP.
Indian J. Biochem. Biophys. 50(1) , 64-71, (2013) The hydrolysis of p-nitrophenyl phosphate (pNPP) by calf intestinal alkaline phosphatase (CIAP) was investigated with respect to kinetic parameters such as V(max), K(m) and K(cat) under varying pH, buffers, substrate concentration, temperature and period of i... |
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Quality assurance in immunoassay performance--comparison of different enzyme immunoassays for the determination of caffeine in consumer products.
Anal. Bioanal. Chem 405(5) , 1601-11, (2013) Enzyme immunoassays with optical detection are amongst the most widely used bioanalytical tools. We defined seven parameters for the quality assessment of immunoassays that were addressed in a systematic study of direct and indirect immunoassays, using the en... |
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Examining the promiscuous phosphatase activity of Pseudomonas aeruginosa arylsulfatase: a comparison to analogous phosphatases.
Proteins 80(4) , 1211-26, (2012) Pseudomonas aeruginosa arylsulfatase (PAS) is a bacterial sulfatase capable of hydrolyzing a range of sulfate esters. Recently, it has been demonstrated to also show very high proficiency for phosphate ester hydrolysis. Such proficient catalytic promiscuity i... |
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Hydrolysis of DNA model substrates catalyzed by metal-substituted Wells-Dawson polyoxometalates.
Dalton Trans. 41(33) , 10028-34, (2012) In this study we report the first example of phosphoester bond hydrolysis in 4-nitrophenyl phosphate (NPP) and bis-4-nitrophenyl phosphate (BNPP), two commonly used DNA model substrates, promoted by metal-substituted polyoxometalates (POMs). Different transit... |
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Utilization of integrated Michaelis-Menten equations for enzyme inhibition diagnosis and determination of kinetic constants using Solver supplement of Microsoft Office Excel.
Comput. Methods Programs Biomed. 109(1) , 26-31, (2013) Enzyme kinetic parameters are usually determined from initial rates nevertheless, laboratory instruments only measure substrate or product concentration versus reaction time (progress curves). To overcome this problem we present a methodology which uses integ... |
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Synergistic stimulation by potassium and ammonium of K(+)-phosphatase activity in gill microsomes from the crab Callinectes ornatus acclimated to low salinity: novel property of a primordial pump.
Arch. Biochem. Biophys. 530(2) , 55-63, (2013) We provide an extensive characterization of the modulation by p-nitrophenylphosphate, Mg²⁺, Na⁺, K(+), Rb⁺, NH(4)(+) and pH of gill microsomal K⁺-phosphatase activity in the posterior gills of Callinectes ornatus acclimated to low salinity (21‰). The synergis... |
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Efficient promotion of phosphate diester cleavage by a face-to-face cyclodextrin dimer without metal.
Chem. Commun. (Camb.) 48(44) , 5515-7, (2012) An organic face-to-face cyclodextrin dimer promotes the cleavage of bis(4-nitrophenyl) phosphate efficiently in neutral pH without the addition of metal. Both of the phosphate diester bonds can be cleaved. |
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GsmR, a response regulator with an HD-related output domain in Xanthomonas campestris, is positively controlled by Clp and is involved in the expression of genes responsible for flagellum synthesis.
FEBS J. 280(1) , 199-213, (2013) In prokaryotes, two-component signal transduction systems, consisting of a histidine kinase and a response regulator, play a critical role in regulating a range of cellular functions. A recent study suggests that XCC3315, a response regulator with a CheY-like... |
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Continuous and sensitive acid phosphatase assay based on a conjugated polyelectrolyte.
ACS Appl. Mater. Interfaces 4(8) , 3784-7, (2012) We report a novel continuous and sensitive fluorescence turn-on assay for ACPs, which consists of a cationic conjugated polyelectrolyte (PPE4+) and a commonly used phosphatase substrate p-nitrophenyl phosphate (pNPP). The kinetics of the ACP catalyzed hydroly... |