ACS Applied Materials & Interfaces 2012-08-01

Continuous and sensitive acid phosphatase assay based on a conjugated polyelectrolyte.

Yonghua Xie, Ying Tan, Renxuan Liu, Rui Zhao, Chunyan Tan, Yuyang Jiang

Index: ACS Appl. Mater. Interfaces 4(8) , 3784-7, (2012)

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Abstract

We report a novel continuous and sensitive fluorescence turn-on assay for ACPs, which consists of a cationic conjugated polyelectrolyte (PPE4+) and a commonly used phosphatase substrate p-nitrophenyl phosphate (pNPP). The kinetics of the ACP catalyzed hydrolysis of the substrate pNPP was monitored by the fluorescence change of PPE4+ and corresponding kinetic parameters were derived to be consistent with the literature reports. The applications of PPE4+/pNPP-based ACP assay in high-throughput screening of ACP inhibitors and detection of prostatic acid phosphotase (PAP) in vitro were demonstrated.